Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY16_RS02130 Genome accession   NZ_CP046832
Coordinates   441445..442452 (+) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus strain 06-2410     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436445..447452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY16_RS02115 - 437278..437550 (-) 273 WP_045595639.1 DUF3624 domain-containing protein -
  GPY16_RS02120 oxyR 437824..438723 (+) 900 WP_045591363.1 DNA-binding transcriptional regulator OxyR -
  GPY16_RS02125 - 438792..441305 (-) 2514 WP_158104941.1 penicillin-binding protein 1A -
  GPY16_RS02130 pilM 441445..442452 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  GPY16_RS02135 pilN 442442..443032 (+) 591 WP_045629546.1 PilN domain-containing protein Machinery gene
  GPY16_RS02140 pilO 443025..443615 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY16_RS02145 pilP 443605..444117 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  GPY16_RS02150 pilQ 444131..445915 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  GPY16_RS02155 aroK 446117..446635 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=408575 GPY16_RS02130 WP_017419656.1 441445..442452(+) (pilM) [Vibrio vulnificus strain 06-2410]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=408575 GPY16_RS02130 WP_017419656.1 441445..442452(+) (pilM) [Vibrio vulnificus strain 06-2410]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACATCTTTGGTGATGTCGCGTCGCAACGAACCACCTTTGGTTAAAGAATTTGCCTATG
GAGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGTGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGACTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment