Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPX85_RS16985 Genome accession   NZ_CP046822
Coordinates   2860523..2862244 (-) Length   573 a.a.
NCBI ID   WP_154169999.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 07-2421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2855523..2867244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX85_RS16965 - 2856229..2857044 (-) 816 WP_004394487.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPX85_RS16970 - 2857113..2858561 (-) 1449 WP_158138959.1 SPOR domain-containing protein -
  GPX85_RS16975 aroB 2858705..2859789 (-) 1085 Protein_2500 3-dehydroquinate synthase -
  GPX85_RS16980 aroK 2859818..2860336 (-) 519 WP_040903956.1 shikimate kinase AroK -
  GPX85_RS16985 pilQ 2860523..2862244 (-) 1722 WP_154169999.1 type IV pilus secretin PilQ Machinery gene
  GPX85_RS16990 pilP 2862269..2862790 (-) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  GPX85_RS16995 pilO 2862780..2863370 (-) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX85_RS17000 pilN 2863363..2863956 (-) 594 WP_158138961.1 PilN domain-containing protein Machinery gene
  GPX85_RS17005 pilM 2863946..2864953 (-) 1008 WP_158138962.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62132.50 Da        Isoelectric Point: 4.8577

>NTDB_id=408427 GPX85_RS16985 WP_154169999.1 2860523..2862244(-) (pilQ) [Vibrio metschnikovii strain 07-2421]
MIKGLPSSIAVVLRYLCITLWLGLALTTQAALASDSNQFKHIDFRVTPDKAAVIVIELASASSVVDVQKTTQGLSIDLLK
TDVAEEQLYLLDVQDFATIVESVEVFRQTPSTLLLAKIHGPFHYDYQLKGRYVEVTITPLKAEVQSAPANLLEPSAKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVILVAPKAELDLREKQQL
EKARLAEELGDLQSAIIKISFAKASDIAEMIGGTGAVNMLSERGSISIDERTNALLIRELPENIHVIREIISALDIPVKQ
VQIEARIVTVNEGNLDELGIRWGVSSTRGNNTVGGSIEGNLTGIGLYQPEDGSEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLQVTPQITPDNRLVL
DLSVTQDRPGDIVKTGTGEAVAIHTQRIGTQVLVNNGETVVLGGIFQHSITNTVDKVPLLGDLPLLGALFRRSYQQMGKS
ELLIFVTPKVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=408427 GPX85_RS16985 WP_154169999.1 2860523..2862244(-) (pilQ) [Vibrio metschnikovii strain 07-2421]
ATGATTAAAGGATTACCTTCAAGTATCGCGGTAGTGCTGCGCTATCTGTGCATCACGCTATGGCTAGGTTTAGCATTGAC
GACGCAGGCTGCTCTAGCGAGTGATAGCAATCAGTTTAAACACATCGATTTTCGGGTAACCCCCGACAAAGCCGCGGTGA
TTGTGATTGAGTTAGCGTCGGCATCGAGTGTGGTTGATGTCCAGAAAACGACGCAGGGGTTGAGTATTGACTTATTAAAA
ACCGATGTGGCTGAGGAGCAGCTTTATTTACTCGATGTCCAAGATTTCGCAACCATAGTTGAGAGTGTGGAAGTTTTTCG
TCAAACACCAAGCACGTTGTTGTTGGCTAAAATACACGGTCCCTTTCATTATGATTATCAGCTCAAAGGTCGTTACGTTG
AAGTGACAATAACGCCGTTAAAAGCCGAGGTTCAAAGTGCGCCAGCCAATCTACTTGAACCATCAGCCAAGCTGATTTCG
ATTAACTTTCAAGACATTCCGGTACGTAACGTTTTACAACTGATCGCTGATTATAATCAGTTTAACTTAGTGGTATCTGA
TTCAGTGGTAGGGAATTTAACCCTGCGTTTAGATGGTGTGCCATGGCAGCAAGTGTTGGACATTATTTTGCAGGTGAAAG
GGTTGGATAAGCGGGTTGAGGGCAATGTGATTTTGGTAGCGCCTAAGGCGGAACTCGATTTACGTGAAAAACAACAGCTG
GAAAAAGCCCGTTTAGCAGAAGAGTTAGGAGACTTGCAGTCAGCCATCATCAAAATAAGTTTCGCGAAGGCGTCAGATAT
CGCTGAGATGATTGGTGGGACGGGAGCGGTGAATATGCTCTCTGAACGAGGTTCAATCAGTATCGATGAACGTACCAATG
CCTTATTAATTCGCGAATTGCCTGAAAATATTCATGTGATCCGAGAGATCATTTCGGCGTTAGATATCCCTGTTAAGCAG
GTACAAATTGAAGCGCGAATTGTGACCGTGAATGAAGGTAATCTTGATGAACTCGGTATTCGTTGGGGCGTGAGCTCAAC
ACGCGGTAATAATACAGTTGGTGGTTCAATAGAAGGTAACTTAACGGGTATTGGTCTCTATCAACCAGAAGATGGTAGCG
AAATGATCAATAACTTCCTTAATGTCAATTTGGGAGCCACAGCAGCGAATGCTTCGAGCATCGCGTTTCAGGTGGCAAAG
CTGGGTTCAGGGACACTGCTCGATCTTGAACTTTCAGCGATGCAGCGAGAATCGAAGGCGGAAATTATTTCCAGTCCACG
ACTTATCACGACCAATAAAAAGCCAGCTTATATAGAGCAGGGGACAGAGATCCCCTATTTAGAATCGTCTTCCAGCGGTG
CGACTACGGTGGCATTTAAGAAAGCGGTACTGAGTTTACAGGTTACCCCGCAGATTACGCCGGATAATCGTCTGGTGCTG
GATTTAAGTGTTACCCAAGACCGACCTGGCGATATTGTCAAAACCGGTACCGGAGAAGCGGTAGCGATCCACACGCAACG
GATTGGTACTCAAGTTTTAGTCAATAATGGCGAAACCGTGGTACTGGGCGGTATTTTCCAACACAGCATCACTAATACGG
TCGATAAAGTGCCATTACTGGGTGATTTACCGTTACTCGGTGCGCTTTTTCGACGTAGCTATCAGCAAATGGGCAAAAGT
GAGCTACTGATTTTTGTTACTCCAAAGGTTGTAATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.776

100

0.773
  pilQ Vibrio cholerae strain A1552

76.776

100

0.773
  pilQ Vibrio campbellii strain DS40M4

76

95.986

0.729
  pilQ Legionella pneumophila strain Lp02

41.852

94.241

0.394
  pilQ Legionella pneumophila strain ERS1305867

41.852

94.241

0.394
  pilQ Pseudomonas aeruginosa PAK

40.647

97.033

0.394
  comQ Acinetobacter baylyi ADP1

39.076

98.255

0.384
  pilQ Acinetobacter baumannii strain A118

36.269

100

0.366
  pilQ Acinetobacter baumannii D1279779

36.269

100

0.366

Multiple sequence alignment