Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPX85_RS16995 Genome accession   NZ_CP046822
Coordinates   2862780..2863370 (-) Length   196 a.a.
NCBI ID   WP_154169997.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 07-2421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857780..2868370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX85_RS16975 aroB 2858705..2859789 (-) 1085 Protein_2500 3-dehydroquinate synthase -
  GPX85_RS16980 aroK 2859818..2860336 (-) 519 WP_040903956.1 shikimate kinase AroK -
  GPX85_RS16985 pilQ 2860523..2862244 (-) 1722 WP_154169999.1 type IV pilus secretin PilQ Machinery gene
  GPX85_RS16990 pilP 2862269..2862790 (-) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  GPX85_RS16995 pilO 2862780..2863370 (-) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX85_RS17000 pilN 2863363..2863956 (-) 594 WP_158138961.1 PilN domain-containing protein Machinery gene
  GPX85_RS17005 pilM 2863946..2864953 (-) 1008 WP_158138962.1 type IV pilus assembly protein PilM Machinery gene
  GPX85_RS17010 - 2865095..2867575 (+) 2481 WP_110167722.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22373.53 Da        Isoelectric Point: 4.5506

>NTDB_id=408429 GPX85_RS16995 WP_154169997.1 2862780..2863370(-) (pilO) [Vibrio metschnikovii strain 07-2421]
MANFRDLDIDELLEWPLNAQLTVIVLVMVVIQVIGYGMYLSPKQEALEGLNQQEQSLKSLVRIKANKVAALPQLQTQLDE
LAERYDFLLRQLPAQQELASLLASVNELGLEHGLTFTRIDWGEKQPQAFLYRLPLNIELTGSYHNIGDFSQAIATLPRII
NVDDVDWQRVSQESSTLHFRVRAYTYQFKAEAGDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=408429 GPX85_RS16995 WP_154169997.1 2862780..2863370(-) (pilO) [Vibrio metschnikovii strain 07-2421]
ATGGCTAATTTTCGTGATTTAGATATCGATGAATTGCTTGAATGGCCGCTCAACGCACAACTGACGGTGATTGTGTTGGT
CATGGTAGTGATTCAAGTCATCGGCTATGGCATGTATTTATCACCTAAACAAGAGGCACTCGAAGGGCTCAATCAGCAAG
AGCAGAGTCTTAAATCATTAGTACGCATTAAGGCCAATAAAGTCGCGGCATTACCTCAGTTGCAAACTCAATTGGATGAG
TTAGCTGAGCGTTATGATTTTTTATTGCGCCAACTCCCAGCACAGCAAGAGCTGGCCAGTTTGCTTGCTTCCGTCAACGA
GCTGGGATTAGAACATGGGTTAACCTTTACTCGAATTGATTGGGGAGAAAAGCAACCGCAGGCATTTTTGTATCGCTTAC
CATTGAATATTGAACTGACGGGAAGTTACCACAACATTGGCGATTTTTCTCAGGCGATAGCCACGCTACCAAGAATCATC
AATGTTGATGATGTGGATTGGCAAAGAGTCAGTCAAGAGAGCAGTACCTTACATTTCAGAGTACGGGCTTACACCTACCA
ATTTAAAGCGGAGGCAGGCGATGAGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

72.449

100

0.724
  pilO Vibrio campbellii strain DS40M4

67.725

96.429

0.653

Multiple sequence alignment