Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX85_RS17005 Genome accession   NZ_CP046822
Coordinates   2863946..2864953 (-) Length   335 a.a.
NCBI ID   WP_158138962.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 07-2421     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2858946..2869953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX85_RS16980 aroK 2859818..2860336 (-) 519 WP_040903956.1 shikimate kinase AroK -
  GPX85_RS16985 pilQ 2860523..2862244 (-) 1722 WP_154169999.1 type IV pilus secretin PilQ Machinery gene
  GPX85_RS16990 pilP 2862269..2862790 (-) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  GPX85_RS16995 pilO 2862780..2863370 (-) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX85_RS17000 pilN 2863363..2863956 (-) 594 WP_158138961.1 PilN domain-containing protein Machinery gene
  GPX85_RS17005 pilM 2863946..2864953 (-) 1008 WP_158138962.1 type IV pilus assembly protein PilM Machinery gene
  GPX85_RS17010 - 2865095..2867575 (+) 2481 WP_110167722.1 penicillin-binding protein 1A -
  GPX85_RS17015 oxyR 2867629..2868528 (-) 900 WP_004394478.1 DNA-binding transcriptional regulator OxyR -
  GPX85_RS17020 - 2868764..2869051 (+) 288 WP_040903944.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37473.09 Da        Isoelectric Point: 7.4417

>NTDB_id=408431 GPX85_RS17005 WP_158138962.1 2863946..2864953(-) (pilM) [Vibrio metschnikovii strain 07-2421]
MSKSLVIGVDIGHHSIKAVALKPNGDNYSLHGCTEIVITDDIFADNDTLNYQKIVKKLKELKKSLPLFSRKVAIALPDTA
VISKVLQIDSDLDPREKEFAIYQAFSHQSPLPMEELSLDFVPLPIQAFAKNRTVTYQVYATRREVVASRLSAIQQAGLEA
TLLDVQLHGLAQIWQQVSQAQQRSNWMLVDVGYTQTTLCIDFADKAPFYKDIALGTRWFELPSLAETMARSDERNQWLFN
RLFVERLTRQLQLFTSVHGQQSLAGIWLSGGGAMMDGLAETITEQLGLPCERFAPLATFVNKITQRKRLNDQPKFTTAAG
LALRGIAWLESGHVA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=408431 GPX85_RS17005 WP_158138962.1 2863946..2864953(-) (pilM) [Vibrio metschnikovii strain 07-2421]
ATGAGTAAATCATTAGTTATTGGTGTCGATATCGGTCATCACAGCATCAAAGCTGTGGCTCTTAAACCAAATGGCGACAA
TTATTCGCTACACGGTTGCACAGAAATCGTCATTACTGACGACATTTTCGCCGATAACGACACGTTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGAGTTTGCCGCTATTTAGTCGCAAGGTTGCGATTGCACTGCCTGACACGGCG
GTAATTAGTAAAGTATTACAAATAGATAGCGACTTAGACCCTCGTGAAAAAGAGTTTGCTATTTATCAGGCTTTTTCTCA
TCAATCGCCGTTGCCGATGGAAGAACTGAGCTTAGATTTTGTTCCTTTACCGATTCAAGCGTTCGCTAAAAACCGAACTG
TGACTTATCAGGTGTATGCCACCCGAAGAGAAGTCGTCGCGAGTCGATTATCGGCGATTCAACAAGCCGGACTGGAAGCA
ACCTTACTGGATGTGCAATTACATGGTCTGGCTCAGATTTGGCAGCAGGTGAGTCAGGCTCAACAGCGCTCGAATTGGAT
GTTGGTCGATGTTGGTTATACCCAAACAACGTTATGTATCGATTTTGCCGATAAAGCGCCGTTTTATAAGGATATTGCCT
TAGGTACACGCTGGTTTGAATTGCCGTCTCTGGCGGAAACGATGGCGCGCAGCGATGAACGTAACCAATGGCTATTCAAT
CGATTATTTGTCGAACGCCTAACCCGTCAACTGCAGTTATTTACTTCCGTTCATGGTCAGCAAAGCCTAGCGGGGATCTG
GTTATCTGGTGGCGGGGCGATGATGGATGGGCTGGCAGAAACCATCACCGAGCAGCTTGGATTACCCTGTGAACGGTTCG
CGCCATTAGCGACTTTTGTCAACAAAATCACTCAACGTAAGCGGTTAAATGATCAGCCTAAATTCACGACGGCTGCTGGT
CTTGCGTTGCGAGGAATAGCATGGTTGGAGAGTGGGCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

60.177

100

0.609
  pilM Vibrio campbellii strain DS40M4

45.97

100

0.46

Multiple sequence alignment