Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY28_RS24835 Genome accession   NZ_CP046814
Coordinates   3319503..3321257 (-) Length   584 a.a.
NCBI ID   WP_053350734.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2010V-1102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3314503..3326257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY28_RS24815 - 3315191..3316030 (-) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPY28_RS24820 - 3316100..3317611 (-) 1512 WP_025768079.1 AAA family ATPase -
  GPY28_RS24825 aroB 3317664..3318764 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  GPY28_RS24830 aroK 3318797..3319315 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY28_RS24835 pilQ 3319503..3321257 (-) 1755 WP_053350734.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY28_RS24840 pilP 3321283..3321798 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  GPY28_RS24845 pilO 3321788..3322381 (-) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY28_RS24850 pilN 3322374..3322949 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  GPY28_RS24855 pilM 3322933..3323949 (-) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63878.88 Da        Isoelectric Point: 4.4593

>NTDB_id=408248 GPY28_RS24835 WP_053350734.1 3319503..3321257(-) (pilQ) [Vibrio alginolyticus strain 2010V-1102]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGGDGGDGGDGDGVGIDDFLNVNLAATSP
NATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVT
PQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGAL
FRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=408248 GPY28_RS24835 WP_053350734.1 3319503..3321257(-) (pilQ) [Vibrio alginolyticus strain 2010V-1102]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGATTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGTGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGTGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
GTGACGGTGGTGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCG
AATGCGACCAGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCGCTGCAACA
AGAGTCTAAAGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAG
AGATTCCTTACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACC
CCGCAAATCACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCAGGACAAGTCGTGAAGACAGG
CACTGGTGAAGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTG
GTGGTATTTTTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGACCTGCCGCTATTAGGTGCTCTT
TTTCGTCGAAGTTATGAGAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(424-582)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.637

100

0.926
  pilQ Vibrio cholerae strain A1552

74.359

100

0.745
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.359

100

0.745
  pilQ Pseudomonas aeruginosa PAK

40.069

99.144

0.397
  pilQ Legionella pneumophila strain Lp02

40.497

96.404

0.39
  pilQ Legionella pneumophila strain ERS1305867

40.497

96.404

0.39
  comQ Acinetobacter baylyi ADP1

37.285

99.658

0.372
  pilQ Acinetobacter baumannii D1279779

35.906

100

0.366
  pilQ Acinetobacter baumannii strain A118

35.906

100

0.366

Multiple sequence alignment