Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY28_RS24845 Genome accession   NZ_CP046814
Coordinates   3321788..3322381 (-) Length   197 a.a.
NCBI ID   WP_053350733.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2010V-1102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3316788..3327381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY28_RS24825 aroB 3317664..3318764 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  GPY28_RS24830 aroK 3318797..3319315 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY28_RS24835 pilQ 3319503..3321257 (-) 1755 WP_053350734.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY28_RS24840 pilP 3321283..3321798 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  GPY28_RS24845 pilO 3321788..3322381 (-) 594 WP_053350733.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY28_RS24850 pilN 3322374..3322949 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  GPY28_RS24855 pilM 3322933..3323949 (-) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  GPY28_RS24860 - 3324094..3326655 (+) 2562 WP_005381325.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0948

>NTDB_id=408250 GPY28_RS24845 WP_053350733.1 3321788..3322381(-) (pilO) [Vibrio alginolyticus strain 2010V-1102]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=408250 GPY28_RS24845 WP_053350733.1 3321788..3322381(-) (pilO) [Vibrio alginolyticus strain 2010V-1102]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAAATTGTGGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGTCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685

Multiple sequence alignment