Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY38_RS15180 Genome accession   NZ_CP046795
Coordinates   3266606..3268330 (+) Length   574 a.a.
NCBI ID   WP_158141366.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2420-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3261606..3273330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY38_RS15160 pilM 3263912..3264916 (+) 1005 WP_158141363.1 type IV pilus assembly protein PilM Machinery gene
  GPY38_RS15165 pilN 3264906..3265496 (+) 591 WP_143680274.1 PilN domain-containing protein Machinery gene
  GPY38_RS15170 pilO 3265489..3266079 (+) 591 WP_158141364.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY38_RS15175 pilP 3266069..3266581 (+) 513 WP_158141365.1 pilus assembly protein PilP Machinery gene
  GPY38_RS15180 pilQ 3266606..3268330 (+) 1725 WP_158141366.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY38_RS15185 aroK 3268518..3269036 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY38_RS15190 aroB 3269064..3270149 (+) 1086 WP_004728570.1 3-dehydroquinate synthase -
  GPY38_RS15195 - 3270195..3271715 (+) 1521 WP_004728571.1 AAA family ATPase -
  GPY38_RS15200 - 3271810..3272640 (+) 831 WP_055465832.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61958.65 Da        Isoelectric Point: 4.4610

>NTDB_id=407928 GPY38_RS15180 WP_158141366.1 3266606..3268330(+) (pilQ) [Vibrio furnissii strain 2420-04]
MIKGLNASTAWGIRCLFVGVCLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLTIELLKT
DVSDDKLYLVDVRDFATAVENIEVFRQQPSTVLVATVQGAFNYEYSLKGNYLEVVISKVSAQEPQPKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNQFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDRREQQQLEK
ARMAEELGDLQSEIIKINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLLRELPDNIHVIRDIVASLDIPVKQVQ
IEARIVTVNEGDLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGSSDSGDGAAIDDFLNVNLTATSANASSIAFQVA
KLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLV
LDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVTSSVDKVPLLGDLPLLGALFRRSYEQMGK
SELLIFVTPKVVIQ

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=407928 GPY38_RS15180 WP_158141366.1 3266606..3268330(+) (pilQ) [Vibrio furnissii strain 2420-04]
ATGATTAAAGGACTAAATGCCTCCACGGCTTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACATTAGCCCAGGAAGCGGCGGCCAATCAGCTGAAAAATATCGACTTTCGCATCAATAAAGACAAAGCGGCGGTGTTGA
TTGTCGAACTGGCGTCGGCTTCTGCGGTGGTGGATGTGCAGAAAACGGAGCAAGGATTGACGATCGAATTGCTTAAAACC
GATGTTAGCGATGACAAACTGTATTTGGTGGACGTGCGCGATTTTGCCACGGCGGTGGAAAACATCGAAGTGTTTCGCCA
GCAACCGAGCACGGTGCTGGTGGCGACGGTGCAAGGGGCATTCAATTACGAATACAGCCTGAAAGGCAATTACTTGGAGG
TGGTGATCAGCAAAGTCAGTGCGCAAGAACCGCAACCGAAAAGTGTCTTAGAAAAAGAGGGCAAGCTGATCTCGATTAAC
TTCCAAGACATTCCCGTGCGCAACGTGCTGCAGCTTATCGCCGACTACAACCAGTTCAACTTGGTGGTCTCCGATTCCGT
CAGCGGCAACTTGACATTGCGCCTCGATGGCGTGCCGTGGCAACAAGTGCTGGATATCATTTTACAAGTCAAAGGGCTGG
ACAAACGGGTCGATGGCAACGTCATTCTGGTGGCGCCCAAAGAGGAGCTGGACCGACGCGAGCAGCAGCAATTGGAAAAA
GCGCGCATGGCGGAAGAGCTGGGCGATCTGCAATCGGAAATCATCAAAATCAATTTCGCCAAGGCATCTGACATTGCACA
GATGATTGGCGGCGATGGGGCGGTGAACATGTTGTCAGAGCGCGGCTCCATCAGTATTGATGAGCGCACCAACGCCTTGC
TACTGCGCGAGCTGCCTGACAACATTCACGTGATCCGCGACATCGTGGCTTCACTCGACATTCCGGTCAAACAAGTGCAA
ATCGAAGCGCGTATCGTCACGGTGAATGAGGGCGATTTGGATGAGCTTGGCGTGCGTTGGGGCTTTAGTTCCACCAACGG
GAGCACCACCACCGGGGGGTCGATTGAAAGTAATCTCGGTGCCATTGGGTTGTATGACGGCAGCAGTGATTCGGGTGATG
GCGCCGCGATTGATGATTTCCTCAATGTGAACCTCACCGCCACCTCCGCCAATGCATCCAGTATTGCGTTTCAAGTGGCC
AAGCTGGGCGCTGACACCTTACTTGATCTTGAATTGTCGGCGATGCAGCGCGAGTCGAAAGCGGAAATTATTTCCAGCCC
AAGGCTGATCACCACCAACAAACAGCCGGCTTACATTGAACAGGGGACGGAAATTCCTTATCTCGAATCGTCCTCCAGTG
GCGCAACCAGCGTGGCATTTAAAAAAGCGGTCCTCAGCCTCAAAGTGACCCCGCAAATCACGCCTGACAACCGTTTGGTG
CTCGATTTGAGCGTCACCCAAGACCGACCGGGTGAAGTGGTGAAAACGGGCACGGGCGAAGCGGTGGCGATTAACACGCA
GCGTATTGGGACCCAAGTGTTAGTTAATAACGGTGAAACCGTGGTGCTGGGCGGCATTTTTCAACACAGCGTGACCAGTT
CTGTGGACAAAGTGCCCTTGCTTGGGGACTTGCCATTACTGGGCGCGCTGTTCCGTCGCAGCTACGAACAGATGGGGAAA
AGTGAGCTACTGATCTTCGTCACGCCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(414-572)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Secretin and TonB N terminus short domain (STN)

(179-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.382

100

0.807
  pilQ Vibrio cholerae strain A1552

80.382

100

0.807
  pilQ Vibrio campbellii strain DS40M4

77.231

95.645

0.739
  comQ Acinetobacter baylyi ADP1

38.737

100

0.395
  pilQ Legionella pneumophila strain ERS1305867

41.392

95.122

0.394
  pilQ Legionella pneumophila strain Lp02

41.392

95.122

0.394
  pilQ Pseudomonas aeruginosa PAK

40.36

96.69

0.39
  pilQ Acinetobacter baumannii D1279779

37.285

100

0.378
  pilQ Acinetobacter baumannii strain A118

37.285

100

0.378

Multiple sequence alignment