Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY38_RS15160 Genome accession   NZ_CP046795
Coordinates   3263912..3264916 (+) Length   334 a.a.
NCBI ID   WP_158141363.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2420-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3258912..3269916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY38_RS15140 - 3259040..3259303 (-) 264 WP_004728560.1 DUF3624 domain-containing protein -
  GPY38_RS15145 - 3259427..3260161 (-) 735 WP_004728561.1 glutathione peroxidase -
  GPY38_RS15150 oxyR 3260305..3261201 (+) 897 WP_014204332.1 DNA-binding transcriptional regulator OxyR -
  GPY38_RS15155 - 3261287..3263767 (-) 2481 WP_038152617.1 PBP1A family penicillin-binding protein -
  GPY38_RS15160 pilM 3263912..3264916 (+) 1005 WP_158141363.1 type IV pilus assembly protein PilM Machinery gene
  GPY38_RS15165 pilN 3264906..3265496 (+) 591 WP_143680274.1 PilN domain-containing protein Machinery gene
  GPY38_RS15170 pilO 3265489..3266079 (+) 591 WP_158141364.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY38_RS15175 pilP 3266069..3266581 (+) 513 WP_158141365.1 pilus assembly protein PilP Machinery gene
  GPY38_RS15180 pilQ 3266606..3268330 (+) 1725 WP_158141366.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY38_RS15185 aroK 3268518..3269036 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36852.19 Da        Isoelectric Point: 6.6617

>NTDB_id=407924 GPY38_RS15160 WP_158141363.1 3263912..3264916(+) (pilM) [Vibrio furnissii strain 2420-04]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSDDIFADNHTLDYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSDLDASEQEFAVYQAFSHQSPFPIDDLSLDFVLSGDNPAGRSASTLYQVYATRREVVDNRLSVASKAGFEP
LLLDVQVHGLLQIWQWVSRAQQRDDWMLVDVGLTQTTLCMDFADKVPFYKDIPLGTRQWHVDPAPHPVPLSESPMAGVNE
LVERIARQVQLLLSLQGHQALGGIWLSGGGATTEGLSAALAARLNLPCELFHPLTGFVTRRRMRARVPDDLQRFSTAAGL
ALRGLNWLERDHGA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=407924 GPY38_RS15160 WP_158141363.1 3263912..3264916(+) (pilM) [Vibrio furnissii strain 2420-04]
ATGGGTAAATCATTAGTTACAGGTGTCGATATCGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAGGGCGAATC
GTATTCGCTGGTCGGCTACCAAGAAATAGTCGTATCCGACGATATTTTCGCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGTTATTTAGTCGCAAAGTTGCGCTGGCTGTGCCGGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGATTTAGATGCCAGCGAACAAGAATTTGCTGTCTATCAGGCGTTTTCCCA
TCAATCGCCGTTTCCCATCGATGATCTGAGTTTGGATTTCGTGTTAAGCGGCGATAACCCCGCTGGGCGCAGTGCCAGCA
CGTTGTATCAGGTATACGCCACGCGACGCGAAGTCGTCGATAACCGGTTATCGGTGGCGAGCAAAGCGGGCTTTGAGCCG
CTGCTGCTCGATGTGCAGGTGCATGGGTTGCTCCAGATTTGGCAATGGGTGAGTCGCGCGCAGCAGCGCGACGATTGGAT
GCTGGTGGACGTCGGGCTGACGCAAACCACGTTATGCATGGATTTCGCCGATAAAGTGCCCTTCTATAAAGACATTCCGC
TGGGAACGCGTCAATGGCATGTCGATCCCGCGCCTCATCCTGTGCCGCTGAGCGAGTCACCGATGGCTGGAGTCAATGAG
CTGGTTGAACGAATTGCGCGCCAAGTGCAACTGCTGTTGTCCTTGCAGGGCCATCAAGCGCTGGGCGGGATTTGGCTGTC
GGGCGGTGGGGCGACAACGGAAGGCCTCAGCGCGGCTTTGGCTGCGCGACTGAATCTGCCGTGTGAACTGTTTCATCCGC
TGACGGGATTTGTGACGCGCCGCCGCATGCGAGCGCGTGTACCAGACGATCTGCAACGTTTTAGTACAGCGGCCGGCTTG
GCGCTGCGCGGACTCAACTGGTTGGAGCGTGACCATGGTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)

Type IV pilus assembly protein PilM; (PilM_2)

(239-324)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.012

100

0.65
  pilM Vibrio campbellii strain DS40M4

45.266

100

0.458

Multiple sequence alignment