Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY38_RS15170 Genome accession   NZ_CP046795
Coordinates   3265489..3266079 (+) Length   196 a.a.
NCBI ID   WP_158141364.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2420-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3260489..3271079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY38_RS15155 - 3261287..3263767 (-) 2481 WP_038152617.1 PBP1A family penicillin-binding protein -
  GPY38_RS15160 pilM 3263912..3264916 (+) 1005 WP_158141363.1 type IV pilus assembly protein PilM Machinery gene
  GPY38_RS15165 pilN 3264906..3265496 (+) 591 WP_143680274.1 PilN domain-containing protein Machinery gene
  GPY38_RS15170 pilO 3265489..3266079 (+) 591 WP_158141364.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY38_RS15175 pilP 3266069..3266581 (+) 513 WP_158141365.1 pilus assembly protein PilP Machinery gene
  GPY38_RS15180 pilQ 3266606..3268330 (+) 1725 WP_158141366.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY38_RS15185 aroK 3268518..3269036 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY38_RS15190 aroB 3269064..3270149 (+) 1086 WP_004728570.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22716.95 Da        Isoelectric Point: 5.0631

>NTDB_id=407926 GPY38_RS15170 WP_158141364.1 3265489..3266079(+) (pilO) [Vibrio furnissii strain 2420-04]
MANVHDLELDEISEWPLVAQLVALVLLVLVLQGAGYWFYLQPKQDTLAAHKQQEQTLKSTIRIKASKVATLPKLKIQLDE
LAQRYDFLLRQLPVQKELASMLASVNELGLDNSLTFTRIDWGEKQNQQFLYRLPLNIELTGNYHDIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEQ

Nucleotide


Download         Length: 591 bp        

>NTDB_id=407926 GPY38_RS15170 WP_158141364.1 3265489..3266079(+) (pilO) [Vibrio furnissii strain 2420-04]
ATGGCTAACGTACACGATCTGGAACTGGACGAAATCAGTGAATGGCCGTTGGTGGCTCAATTGGTCGCCTTGGTGCTGCT
GGTGTTGGTGTTGCAGGGCGCGGGGTACTGGTTTTATTTGCAGCCTAAACAAGACACATTGGCCGCACACAAGCAGCAAG
AGCAAACGCTGAAGTCGACGATTCGCATCAAAGCCAGCAAAGTCGCGACATTGCCGAAGCTCAAGATTCAGCTGGATGAA
CTGGCACAGCGGTACGACTTCCTGTTGCGTCAGCTACCGGTGCAAAAAGAGCTCGCCAGCATGCTGGCGTCGGTGAACGA
GTTGGGGCTCGACAACAGCCTGACTTTTACTCGCATCGATTGGGGGGAAAAGCAGAATCAGCAATTTCTCTATCGGCTGC
CGCTCAATATCGAATTGACCGGTAATTATCACGACATCGGCGATTTTTCTGAAGCGATCGCGCGTCTACCGCGCATCATT
AATTTTGACGACGTTGACTGGCAGCGCGTCAGCCAAGAGAGCAGCACGCTCCATTTCCGCGTTCGCGCCTATACCTATCA
GTTCAAACCGGAGGTCAGTGATGAACAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

78.061

100

0.781
  pilO Vibrio campbellii strain DS40M4

70.526

96.939

0.684

Multiple sequence alignment