Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY10_RS11235 Genome accession   NZ_CP046767
Coordinates   2401692..2403428 (+) Length   578 a.a.
NCBI ID   WP_233275337.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2013V-1300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2396692..2408428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY10_RS11215 pilM 2398998..2400002 (+) 1005 WP_158151663.1 type IV pilus assembly protein PilM Machinery gene
  GPY10_RS11220 pilN 2399992..2400582 (+) 591 WP_158151664.1 PilN domain-containing protein Machinery gene
  GPY10_RS11225 pilO 2400575..2401165 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY10_RS11230 pilP 2401155..2401667 (+) 513 WP_158151665.1 pilus assembly protein PilP Machinery gene
  GPY10_RS11235 pilQ 2401692..2403428 (+) 1737 WP_233275337.1 type IV pilus secretin PilQ Machinery gene
  GPY10_RS11240 aroK 2403616..2404134 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY10_RS11245 aroB 2404162..2405247 (+) 1086 WP_024374091.1 3-dehydroquinate synthase -
  GPY10_RS11250 - 2405293..2406801 (+) 1509 WP_158151666.1 AAA family ATPase -
  GPY10_RS11255 - 2406896..2407726 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62409.36 Da        Isoelectric Point: 4.5625

>NTDB_id=407426 GPY10_RS11235 WP_233275337.1 2401692..2403428(+) (pilQ) [Vibrio fluvialis strain 2013V-1300]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLQ
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=407426 GPY10_RS11235 WP_233275337.1 2401692..2403428(+) (pilQ) [Vibrio fluvialis strain 2013V-1300]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTTGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGATGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGCAA
AAAGCCCGCATGGCCGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TGCTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGTTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAACGTTAATCTCGCTGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCCGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCGAGACTGATCACCACCAACAAGCAGCCGGCGTATATTGAACAGGGTACTGAGATTCCGTACCTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTGTTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGCACTGGCGAGGCGGTGGC
GATCAACACGCAACGTATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCCGTGTTAGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTTGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.52

100

0.817
  pilQ Vibrio cholerae strain A1552

81.52

100

0.817
  pilQ Vibrio campbellii strain DS40M4

79.053

94.983

0.751
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.541

96.021

0.389
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382

Multiple sequence alignment