Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPY10_RS11220 Genome accession   NZ_CP046767
Coordinates   2399992..2400582 (+) Length   196 a.a.
NCBI ID   WP_158151664.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2013V-1300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2394992..2405582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY10_RS11205 oxyR 2395418..2396314 (+) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GPY10_RS11210 - 2396375..2398855 (-) 2481 WP_158151662.1 penicillin-binding protein 1A -
  GPY10_RS11215 pilM 2398998..2400002 (+) 1005 WP_158151663.1 type IV pilus assembly protein PilM Machinery gene
  GPY10_RS11220 pilN 2399992..2400582 (+) 591 WP_158151664.1 PilN domain-containing protein Machinery gene
  GPY10_RS11225 pilO 2400575..2401165 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY10_RS11230 pilP 2401155..2401667 (+) 513 WP_158151665.1 pilus assembly protein PilP Machinery gene
  GPY10_RS11235 pilQ 2401692..2403428 (+) 1737 WP_233275337.1 type IV pilus secretin PilQ Machinery gene
  GPY10_RS11240 aroK 2403616..2404134 (+) 519 WP_004728569.1 shikimate kinase AroK -
  GPY10_RS11245 aroB 2404162..2405247 (+) 1086 WP_024374091.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22244.66 Da        Isoelectric Point: 8.9705

>NTDB_id=407423 GPY10_RS11220 WP_158151664.1 2399992..2400582(+) (pilN) [Vibrio fluvialis strain 2013V-1300]
MLHNINLIPWREAQREAHKRRFVGLCVLALMVALGVQWGAGFYLDQQTQAQQLRLAFLNQHIHQLDAQIAELKVTEQEHK
ALLTRLNIVESLQQKRNKTTEFMDLMPQLIPEGVYVDKIKMNGEEIEISGISDSTARLATMLDNLEKSTQLSEVGMHSIV
SGNRRFGKQFQSFKVSFLFHASAHKPLATVTGEKHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=407423 GPY10_RS11220 WP_158151664.1 2399992..2400582(+) (pilN) [Vibrio fluvialis strain 2013V-1300]
ATGCTGCATAACATCAACCTTATTCCCTGGCGTGAAGCGCAGCGCGAGGCGCACAAACGCCGCTTCGTCGGACTGTGCGT
GCTCGCGTTGATGGTGGCATTGGGCGTGCAGTGGGGCGCTGGCTTTTATCTCGATCAGCAAACTCAGGCTCAGCAGCTGC
GACTGGCATTTCTCAATCAGCACATTCACCAACTGGATGCGCAGATTGCCGAACTGAAAGTGACCGAGCAAGAGCATAAA
GCGCTGCTGACCCGGCTCAATATCGTCGAATCGTTACAGCAAAAACGCAATAAGACCACCGAATTCATGGACCTGATGCC
GCAGCTGATTCCGGAAGGTGTCTACGTCGACAAGATCAAAATGAATGGCGAAGAAATTGAAATCAGCGGCATCAGTGACA
GTACTGCCCGACTCGCCACCATGCTCGACAATCTGGAAAAATCGACCCAACTGTCTGAGGTCGGTATGCATTCGATCGTG
TCAGGTAATCGACGTTTTGGTAAACAATTTCAGAGCTTTAAGGTGTCATTTCTGTTTCATGCGTCAGCTCATAAACCGCT
CGCCACCGTGACAGGAGAGAAACATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

68.617

95.918

0.658
  pilN Vibrio campbellii strain DS40M4

60.714

100

0.607

Multiple sequence alignment