Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY10_RS11215 Genome accession   NZ_CP046767
Coordinates   2398998..2400002 (+) Length   334 a.a.
NCBI ID   WP_158151663.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2013V-1300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2393998..2405002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY10_RS11195 - 2394149..2394412 (-) 264 WP_020429299.1 DUF3624 domain-containing protein -
  GPY10_RS11200 - 2394541..2395275 (-) 735 WP_061057153.1 glutathione peroxidase -
  GPY10_RS11205 oxyR 2395418..2396314 (+) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GPY10_RS11210 - 2396375..2398855 (-) 2481 WP_158151662.1 penicillin-binding protein 1A -
  GPY10_RS11215 pilM 2398998..2400002 (+) 1005 WP_158151663.1 type IV pilus assembly protein PilM Machinery gene
  GPY10_RS11220 pilN 2399992..2400582 (+) 591 WP_158151664.1 PilN domain-containing protein Machinery gene
  GPY10_RS11225 pilO 2400575..2401165 (+) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY10_RS11230 pilP 2401155..2401667 (+) 513 WP_158151665.1 pilus assembly protein PilP Machinery gene
  GPY10_RS11235 pilQ 2401692..2403428 (+) 1737 WP_233275337.1 type IV pilus secretin PilQ Machinery gene
  GPY10_RS11240 aroK 2403616..2404134 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36853.17 Da        Isoelectric Point: 6.6595

>NTDB_id=407422 GPY10_RS11215 WP_158151663.1 2398998..2400002(+) (pilM) [Vibrio fluvialis strain 2013V-1300]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRSDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQE
LVERLARQIQLFLSLQGHQPLAGIWLSGGGAATEGLAQAISERLALPCELFNPLAEFTCRASLRHHPQVDLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=407422 GPY10_RS11215 WP_158151663.1 2398998..2400002(+) (pilM) [Vibrio fluvialis strain 2013V-1300]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGGTCTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTTACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCTCCGATTGGAT
GCTGGTCGATGTCGGGTTCAGCCAAACCTCATTGTGCATCGATTTTGCCGATAAAACGCCTTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAG
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTGTCTCTGCAAGGCCATCAACCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGCTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCACCATCCTCAGGTTGATTTGCAGCGTTTCAGTACTGCGGCCGGCTTG
GCGCTGCGCGGGCTGAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.68

100

0.665
  pilM Vibrio campbellii strain DS40M4

48.116

100

0.497

Multiple sequence alignment