Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPJ81_RS24615 Genome accession   NZ_CP046621
Coordinates   5442432..5443703 (-) Length   423 a.a.
NCBI ID   WP_157194816.1    Uniprot ID   A0A6I6GZU1
Organism   Pseudomonas alkylphenolica strain Neo     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5437432..5448703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPJ81_RS24600 (GPJ81_24590) - 5439091..5440695 (-) 1605 WP_157194556.1 SPOR domain-containing protein -
  GPJ81_RS24605 (GPJ81_24595) aroB 5440712..5441809 (-) 1098 WP_157194557.1 3-dehydroquinate synthase -
  GPJ81_RS24610 (GPJ81_24600) aroK 5441909..5442427 (-) 519 WP_038615538.1 shikimate kinase AroK -
  GPJ81_RS24615 (GPJ81_24605) pilQ 5442432..5443703 (-) 1272 WP_157194816.1 type IV pilus secretin PilQ Machinery gene
  GPJ81_RS24620 (GPJ81_24610) - 5443746..5444672 (-) 927 WP_157194558.1 pilus assembly protein PilP -
  GPJ81_RS24625 (GPJ81_24615) - 5444669..5445187 (-) 519 WP_198325385.1 PilN domain-containing protein -
  GPJ81_RS24630 (GPJ81_24620) pilM 5445184..5446110 (-) 927 WP_157194560.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45712.28 Da        Isoelectric Point: 4.6947

>NTDB_id=406107 GPJ81_RS24615 WP_157194816.1 5442432..5443703(-) (pilQ) [Pseudomonas alkylphenolica strain Neo]
MALFAGLFTLQAACAVSASSAPPGEPLSLNFQDVEVRSVLQVLADFSGVNLVASDAVQGRVTLRLQDVPWEQALDLVLRS
KGLERRTEGNVLLVAPIGELGGQERQLLESRQQVAGLVTTRRELLPIHYAKAADIAAVVQSLTENDPFDAAAGALGVDER
TNTLIARQTPERLEELRQLIARLDVPVRQVMIEARIVEANVDYERSLGVRWGGELDLGSGLVLGGADTFVDLGAERAGSA
IGLGLVRDNVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSGSGATSVTFREASLSLEVTPQITPD
NRVIMAVKVTKDEPDYLNALAQVPPIRKNEVNAKVRVKDGETIVIGGVYSTVQSKVVDKVPFFGDVPYVGRLFRRDVLQE
KKSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=406107 GPJ81_RS24615 WP_157194816.1 5442432..5443703(-) (pilQ) [Pseudomonas alkylphenolica strain Neo]
ATGGCGCTGTTCGCCGGGTTGTTTACCCTGCAGGCTGCTTGCGCGGTGTCGGCCAGTAGCGCGCCGCCGGGCGAGCCTTT
GTCGTTGAACTTCCAGGATGTCGAGGTGCGTTCGGTGTTGCAGGTGTTGGCTGATTTTTCCGGGGTCAACCTGGTTGCCA
GCGATGCGGTGCAGGGCCGGGTCACCCTGCGCCTGCAGGATGTGCCCTGGGAGCAGGCGCTGGACCTGGTGCTGCGCAGC
AAGGGGCTGGAACGGCGTACCGAAGGCAATGTCCTGCTGGTGGCGCCCATTGGCGAGCTTGGCGGTCAGGAGCGGCAGTT
GCTGGAAAGTCGTCAGCAGGTGGCCGGCCTGGTGACGACGCGCCGAGAGCTGCTGCCGATTCACTATGCCAAGGCGGCAG
ACATCGCGGCAGTGGTCCAGTCGCTGACGGAGAATGATCCATTCGACGCCGCTGCGGGGGCGCTGGGTGTCGATGAGCGA
ACCAATACCCTGATCGCGCGCCAGACCCCGGAGCGGCTGGAGGAATTACGCCAGTTGATCGCTCGCCTGGATGTACCGGT
GCGCCAGGTGATGATCGAAGCGCGGATCGTTGAAGCGAATGTCGACTACGAAAGGAGCCTGGGCGTGCGCTGGGGCGGGG
AGCTGGATCTGGGCAGTGGTCTGGTCCTGGGTGGCGCCGACACCTTTGTCGACCTGGGGGCGGAGCGGGCTGGCTCGGCC
ATCGGCCTGGGACTGGTGCGCGACAATGTCCTGCTCGACCTGGAACTCAGTGCCATGGAGAAGTCCGGTAATGGTGAAAT
CATCTCCCAGCCCAAAGTGGTCACCGCCGACAAGGAAACCGCGCGAATCCTCAAGGGCACCGAAGTGCCGTATCAGGAAA
CCAGTGGCAGCGGCGCTACCTCCGTGACCTTCCGCGAGGCGTCGCTGTCGTTGGAGGTGACGCCGCAGATCACGCCGGAC
AACCGGGTGATCATGGCCGTGAAGGTGACCAAGGATGAGCCGGACTACCTCAATGCCCTGGCCCAGGTGCCGCCGATTCG
CAAGAACGAGGTCAATGCCAAGGTCCGCGTGAAGGACGGCGAGACCATCGTGATCGGTGGCGTGTACTCGACGGTGCAAA
GTAAAGTTGTCGACAAGGTGCCATTTTTCGGCGATGTGCCGTATGTTGGGCGGCTGTTTCGTCGCGATGTTCTACAAGAG
AAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(124-189)

Secretin and TonB N terminus short domain (STN)

(50-95)

Bacterial type II and III secretion system protein (Secretin)

(258-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6GZU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

61.521

100

0.631
  comQ Acinetobacter baylyi ADP1

42.022

100

0.442
  pilQ Acinetobacter baumannii strain A118

40.13

100

0.437
  pilQ Acinetobacter baumannii D1279779

40.13

100

0.437
  pilQ Legionella pneumophila strain ERS1305867

43.51

98.345

0.428
  pilQ Legionella pneumophila strain Lp02

43.51

98.345

0.428
  pilQ Vibrio campbellii strain DS40M4

42.317

100

0.423
  pilQ Vibrio cholerae strain A1552

42.41

98.109

0.416
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

98.109

0.416
  pilQ Neisseria meningitidis MC58

36.512

100

0.371
  pilQ Neisseria meningitidis M1080

37.019

98.345

0.364

Multiple sequence alignment