Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GNH96_RS08015 Genome accession   NZ_CP046565
Coordinates   1653479..1654693 (+) Length   404 a.a.
NCBI ID   WP_169603200.1    Uniprot ID   A0A858Q7W9
Organism   Methylococcus geothermalis strain IM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1648479..1659693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNH96_RS08005 (GNH96_07975) - 1648944..1651709 (+) 2766 WP_169603198.1 valine--tRNA ligase -
  GNH96_RS08010 (GNH96_07980) pilB 1651759..1653474 (+) 1716 WP_169603199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GNH96_RS08015 (GNH96_07985) pilC 1653479..1654693 (+) 1215 WP_169603200.1 type II secretion system F family protein Machinery gene
  GNH96_RS08020 (GNH96_07990) pilD 1654704..1655582 (+) 879 WP_169603201.1 A24 family peptidase Machinery gene
  GNH96_RS08025 (GNH96_07995) coaE 1655590..1656186 (+) 597 WP_169603202.1 dephospho-CoA kinase -
  GNH96_RS08030 (GNH96_08000) zapD 1656289..1657056 (+) 768 WP_169603203.1 cell division protein ZapD -
  GNH96_RS08035 (GNH96_08005) yacG 1657068..1657283 (+) 216 WP_169603204.1 DNA gyrase inhibitor YacG -
  GNH96_RS08040 (GNH96_08010) cysG 1657295..1658722 (-) 1428 WP_169603205.1 siroheme synthase CysG -
  GNH96_RS08045 (GNH96_08015) - 1658770..1659000 (+) 231 WP_169603206.1 sulfurtransferase TusA family protein -
  GNH96_RS08050 (GNH96_08020) - 1659008..1659670 (-) 663 WP_169603207.1 HAD family phosphatase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43921.57 Da        Isoelectric Point: 9.6429

>NTDB_id=405585 GNH96_RS08015 WP_169603200.1 1653479..1654693(+) (pilC) [Methylococcus geothermalis strain IM1]
MASQETALFVWEGLDRNGSRTKGEVSARSEITARAELRRQGIRVVKIKKKPKPLFGGPRKKITSKHIAVFSRQLATMLSA
GVPLVQAFEIVGRGHDNPAMQDLLLGIKADVESGTTLADALSKHPVHFDELFCNLVRAGEQAGVLETLLHKVADYKEKTE
SLKGKIKKALTYPIAVVVVAVIVTTILLMFVVPTFEDLFKSFGADLPAFTQLVIDLSRWLRDWWYVVFGSAAVSATAFIK
ARQRSPAFNHLLDRMALAVPVVGAILHKAAVARFARTLSTMSAAGVPLVEALQSVAGATGNIIYGEAVMRMREDVAIGQQ
LQMSMRQANLFPNMVMQMVAIGEESGSLDSMLSKVADFYEEEVDNAVDSLSSLLEPLIMVVLGVLVGGLVIAMYLPIFKL
GSVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=405585 GNH96_RS08015 WP_169603200.1 1653479..1654693(+) (pilC) [Methylococcus geothermalis strain IM1]
GTGGCGAGCCAGGAAACGGCGTTGTTCGTCTGGGAGGGACTGGACCGCAATGGCAGCCGGACCAAGGGAGAAGTCAGCGC
CCGCTCGGAGATCACTGCGAGGGCGGAGCTGAGGCGGCAGGGCATCCGCGTCGTCAAGATCAAGAAGAAGCCCAAACCCC
TGTTCGGCGGTCCGCGCAAGAAAATCACGTCGAAGCACATCGCCGTGTTCAGCCGCCAGTTGGCGACCATGCTGTCGGCG
GGGGTGCCCTTGGTGCAGGCTTTCGAAATCGTGGGCCGCGGCCATGACAACCCGGCCATGCAGGACCTCCTGCTGGGCAT
CAAGGCCGACGTCGAGAGCGGCACGACCCTGGCGGACGCCCTGTCGAAACACCCGGTCCATTTCGACGAGTTGTTCTGCA
ACCTGGTGCGGGCCGGCGAGCAGGCCGGCGTGCTGGAAACCCTGTTGCACAAGGTGGCCGATTACAAGGAAAAGACCGAG
TCCCTCAAGGGCAAGATCAAGAAGGCACTCACCTATCCGATCGCGGTCGTCGTCGTTGCGGTGATCGTGACCACGATCCT
GCTCATGTTCGTGGTGCCGACCTTCGAAGACCTGTTCAAGAGCTTCGGTGCCGATCTGCCTGCCTTCACCCAGCTGGTGA
TCGATCTTTCGCGCTGGCTGCGGGACTGGTGGTACGTCGTCTTCGGAAGCGCCGCGGTTTCGGCGACCGCCTTCATCAAG
GCCAGGCAGCGATCGCCTGCGTTCAACCATTTGCTGGACCGGATGGCCCTCGCGGTGCCGGTGGTCGGGGCCATTCTGCA
CAAAGCCGCCGTCGCCCGCTTCGCCCGGACCCTGTCCACGATGTCCGCCGCCGGCGTGCCCCTGGTCGAGGCATTGCAAT
CGGTGGCCGGAGCGACCGGAAACATCATTTACGGCGAGGCGGTCATGCGCATGCGCGAAGACGTAGCCATCGGCCAGCAG
CTCCAGATGTCGATGCGCCAGGCCAATCTGTTCCCCAACATGGTGATGCAGATGGTGGCGATCGGGGAGGAGTCGGGATC
GCTCGACAGCATGCTGTCGAAAGTCGCCGATTTCTACGAGGAAGAAGTGGACAATGCCGTAGACTCGTTGAGTAGCCTGC
TGGAGCCTTTGATCATGGTGGTCCTCGGGGTATTGGTGGGCGGGCTCGTGATCGCGATGTATCTGCCGATTTTCAAACTG
GGTTCCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858Q7W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

60.606

98.02

0.594
  pilC Pseudomonas stutzeri DSM 10701

59.25

99.01

0.587
  pilC Acinetobacter baumannii D1279779

57.816

99.752

0.577
  pilC Acinetobacter baylyi ADP1

56.618

100

0.572
  pilG Neisseria meningitidis 44/76-A

46.851

98.267

0.46
  pilG Neisseria gonorrhoeae MS11

46.851

98.267

0.46
  pilC Vibrio campbellii strain DS40M4

44.361

98.762

0.438
  pilC Vibrio cholerae strain A1552

43.939

98.02

0.431
  pilC Thermus thermophilus HB27

40.602

98.762

0.401

Multiple sequence alignment