Detailed information NTG0405586

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GNH96_RS08020
• Genome accession: NZ_CP046565
• Coordinates: 1654704..1655582 (+)
• Length: 292 a.a.
• NCBI ID: WP_169603201.1
• Uniprot ID: -
• Organism: Methylococcus geothermalis strain IM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1649704..1660582

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GNH96_RS08010 (GNH96_07980)	pilB	1651759..1653474 (+)	1716	WP_169603199.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GNH96_RS08015 (GNH96_07985)	pilC	1653479..1654693 (+)	1215	WP_169603200.1	type II secretion system F family protein	Machinery gene
GNH96_RS08020 (GNH96_07990)	pilD	1654704..1655582 (+)	879	WP_169603201.1	A24 family peptidase	Machinery gene
GNH96_RS08025 (GNH96_07995)	coaE	1655590..1656186 (+)	597	WP_169603202.1	dephospho-CoA kinase	-
GNH96_RS08030 (GNH96_08000)	zapD	1656289..1657056 (+)	768	WP_169603203.1	cell division protein ZapD	-
GNH96_RS08035 (GNH96_08005)	yacG	1657068..1657283 (+)	216	WP_169603204.1	DNA gyrase inhibitor YacG	-
GNH96_RS08040 (GNH96_08010)	cysG	1657295..1658722 (-)	1428	WP_169603205.1	siroheme synthase CysG	-
GNH96_RS08045 (GNH96_08015)	-	1658770..1659000 (+)	231	WP_169603206.1	sulfurtransferase TusA family protein	-
GNH96_RS08050 (GNH96_08020)	-	1659008..1659670 (-)	663	WP_169603207.1	HAD family phosphatase	-
GNH96_RS08055 (GNH96_08025)	-	1659860..1660435 (+)	576	WP_169603208.1	RNA pyrophosphohydrolase	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31554.25 Da
• Isoelectric Point: 6.9999

>NTDB_id=405586 GNH96_RS08020 WP_169603201.1 1654704..1655582(+) (pilD) [Methylococcus geothermalis strain IM1]
MTDSTLLPEGPALIAICGLFGLIIGSFLNVVIHRLPQMMQRAWRRECREFLEPGAELPSGGADYNLWRPGSQCPHCGAPV
RFWQNVPVVSFLWLRGRCAACGAAISRRYPLVELLTGVLFAMVAWHFGASWQTLAALSLTAGLIALAFIDLDHLVLPDDI
TLPLLWLGLLLNAQSLFCSLETALYGTVAGYLVLWTLHHGFLLLTGREGMGFGDFKLLAMLGAWLGWPMLPMIILFSSIS
GAVIGSIALAAGGKDGRTPIPFGPFLAAGGWIALLWGPALNDAYWRWAAPGG

Nucleotide

• Length: 879 bp

>NTDB_id=405586 GNH96_RS08020 WP_169603201.1 1654704..1655582(+) (pilD) [Methylococcus geothermalis strain IM1]
TTGACCGACTCGACATTGTTGCCGGAGGGACCGGCTCTGATCGCCATCTGCGGCCTGTTCGGACTGATCATCGGCAGTTT
TCTCAACGTGGTCATCCATCGCCTGCCACAGATGATGCAGCGGGCCTGGCGCAGGGAATGCCGGGAATTCCTGGAACCCG
GCGCCGAGCTACCGTCCGGGGGAGCCGACTACAACCTCTGGAGGCCGGGGTCGCAGTGTCCGCATTGCGGAGCGCCGGTC
CGGTTCTGGCAGAACGTGCCGGTAGTGAGCTTTTTGTGGCTTCGTGGCCGCTGTGCCGCCTGCGGCGCGGCGATCTCCCG
GCGTTATCCGCTGGTCGAACTGCTGACCGGAGTGCTGTTCGCGATGGTGGCCTGGCATTTCGGCGCATCCTGGCAAACCT
TGGCTGCCTTGAGCCTGACTGCCGGCCTGATCGCTTTGGCCTTCATCGACCTCGACCATCTGGTGCTGCCGGACGATATC
ACGCTTCCCCTCCTCTGGCTCGGCCTGCTCCTGAATGCCCAGAGCCTCTTCTGCAGCCTGGAGACGGCGCTGTACGGGAC
GGTCGCCGGTTATCTCGTCCTATGGACCCTACATCACGGTTTTCTGCTGCTGACCGGAAGGGAGGGGATGGGCTTCGGCG
ATTTCAAGCTGCTCGCCATGCTGGGTGCCTGGCTGGGCTGGCCGATGCTGCCGATGATCATTCTGTTTTCATCGATCTCC
GGCGCGGTGATCGGTTCGATTGCGCTGGCGGCCGGAGGGAAGGACGGGCGTACGCCGATTCCGTTCGGCCCGTTTCTGGC
GGCGGGAGGCTGGATCGCCCTGCTGTGGGGGCCCGCGCTCAACGACGCCTACTGGCGCTGGGCCGCGCCGGGAGGCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-127)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	49.129	98.288	0.483
pilD	Vibrio cholerae strain A1552	50.916	93.493	0.476
pilD	Vibrio campbellii strain DS40M4	48.175	93.836	0.452
pilD	Acinetobacter baumannii D1279779	46.886	93.493	0.438
pilD	Acinetobacter nosocomialis M2	46.52	93.493	0.435