Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GNH96_RS08010 Genome accession   NZ_CP046565
Coordinates   1651759..1653474 (+) Length   571 a.a.
NCBI ID   WP_169603199.1    Uniprot ID   A0A858Q7T5
Organism   Methylococcus geothermalis strain IM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1646759..1658474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNH96_RS07995 (GNH96_07965) - 1646928..1648421 (+) 1494 WP_169603196.1 leucyl aminopeptidase -
  GNH96_RS08000 (GNH96_07970) - 1648418..1648849 (+) 432 WP_169603197.1 DNA polymerase III subunit chi -
  GNH96_RS08005 (GNH96_07975) - 1648944..1651709 (+) 2766 WP_169603198.1 valine--tRNA ligase -
  GNH96_RS08010 (GNH96_07980) pilB 1651759..1653474 (+) 1716 WP_169603199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GNH96_RS08015 (GNH96_07985) pilC 1653479..1654693 (+) 1215 WP_169603200.1 type II secretion system F family protein Machinery gene
  GNH96_RS08020 (GNH96_07990) pilD 1654704..1655582 (+) 879 WP_169603201.1 A24 family peptidase Machinery gene
  GNH96_RS08025 (GNH96_07995) coaE 1655590..1656186 (+) 597 WP_169603202.1 dephospho-CoA kinase -
  GNH96_RS08030 (GNH96_08000) zapD 1656289..1657056 (+) 768 WP_169603203.1 cell division protein ZapD -
  GNH96_RS08035 (GNH96_08005) yacG 1657068..1657283 (+) 216 WP_169603204.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63132.90 Da        Isoelectric Point: 5.7879

>NTDB_id=405584 GNH96_RS08010 WP_169603199.1 1651759..1653474(+) (pilB) [Methylococcus geothermalis strain IM1]
MAAVPGVQQLSGLAKSLVKAEMLSEADAQAYFEDARRKKTPFVSYLVSNKILDSLKIAQVAHQEFGLPLLDLDAVAFEIP
PTQWVSEKLIRHHHILPLMRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCILVEEDKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDDGPHADPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPAALGVK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPGSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVILIMAQRLARRLCPHCKREEKVPPEVLLA
SGFREGDIGRFTLYGPAGCEQCVKGYKGRVGIYQVMPISEEINRIILEGGNVMTLTEQAHAEGIADLRESGLKKVKAGVT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=405584 GNH96_RS08010 WP_169603199.1 1651759..1653474(+) (pilB) [Methylococcus geothermalis strain IM1]
ATGGCTGCGGTTCCCGGCGTCCAGCAGCTGAGCGGTCTGGCGAAGAGCCTGGTCAAGGCCGAGATGCTTTCCGAAGCGGA
TGCCCAGGCCTATTTCGAGGATGCGCGCCGGAAGAAGACCCCTTTCGTCAGTTATCTGGTCTCCAACAAGATCCTCGACA
GCCTTAAGATTGCTCAGGTCGCCCACCAGGAGTTCGGGCTGCCGCTGCTCGACCTGGACGCGGTCGCTTTCGAGATTCCG
CCGACCCAGTGGGTCAGCGAAAAGCTGATACGCCACCACCACATCCTTCCGTTGATGCGGCGCGGCAACCATCTGTTCGT
CGCGGTGTCGGATCCGACCAATTTTCTGGGGCTGGACGAAATCACCTTCCAGACCCGCCTCGTCACCGATTGCATCCTGG
TCGAAGAGGACAAACTCTCCCGCTGCATCGAAGCAGTGCTGGATGCGGCAGATACCAGCCTCAAGCAGTTGCTCGAGGAG
GACCTGGACAGTCTCCAGATCACCGCCGGAGACGACGATGGCCCGCACGCCGATCCGGAAATTTCCGGCATCGACGACGC
GCCGATCGTGCGTTTCGTCAACCGCCTCCTGCTGGATGCGGTGAGAAAAGGGGCTTCGGACATTCATATCGAACCCTACG
AAAAATTCCTGCGCATCCGGTTCCGGCACGACGGCATCCTGCACGAAGTGGCGAACCCTCCCGCGGCGCTGGGAGTCAAG
ATCTGCGCCCGCATCAAGGTGATGTCGCGCATGGACATCGCAGAACACCGGATCCCCCAGGATGGCCGCATCAAGATGAC
GTTGTCGCGTTCCCGCGCCATCGATTTCCGTGTCAATACCTGCCCGACACTGTTCGGGGAAAAGATCGTGCTGCGTATCC
TCGATCCGGGTTCGGCCCAGATCGGCGTCGAAATGCTGGGATTCGAACCGGAGCAGCGGGACAATTTCCTGCGCGCCCTG
GAAAAGCCCTACGGCATGATCCTCGTCACCGGTCCCACCGGCAGCGGCAAGACCGTCACTCTGTACACCGGCCTGAACCT
GCTCAATCGTGTCGAAGCCAACATTTCCACCGTGGAGGACCCGGTCGAAATCACGGTTCCGGGGATCAACCAGGTCAACG
TCAACTACAAGACCGGCCTCACTTTCGCCGAGGCGCTGCGCGCGTTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCGGCCCAGACCGGCCATCTGGTCCTCTCGACCCTGCACAC
CAACGATGCCCCGCAGACCTTGAACCGCCTGATGCAGATGGGTATCCCGGCCTTCAACATCGCCTCCTCGGTCATCTTGA
TCATGGCGCAGCGGCTGGCCAGGCGGCTTTGTCCGCATTGCAAGCGGGAGGAAAAGGTGCCGCCCGAAGTCCTGCTCGCA
TCCGGCTTCCGCGAGGGCGATATCGGCAGGTTCACGCTCTATGGACCAGCCGGCTGCGAGCAGTGCGTGAAGGGCTACAA
AGGCCGCGTCGGCATCTACCAGGTCATGCCGATTTCCGAGGAAATCAACCGTATCATCCTGGAAGGCGGAAATGTGATGA
CCCTGACCGAGCAGGCGCACGCGGAGGGTATCGCGGACCTGAGGGAGTCCGGGCTGAAGAAGGTCAAGGCAGGCGTCACC
AGCCTTGAGGAAATCGACCGCGTGACGAGGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858Q7T5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56

100

0.564
  pilB Acinetobacter baylyi ADP1

52.414

100

0.532
  pilB Acinetobacter baumannii D1279779

51.641

100

0.524
  pilB Vibrio cholerae strain A1552

48.325

99.299

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

47.09

99.299

0.468
  pilF Neisseria gonorrhoeae MS11

46.986

98.774

0.464
  pilB Vibrio campbellii strain DS40M4

46.127

99.475

0.459
  pilF Thermus thermophilus HB27

37.937

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.602

93.17

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.256

98.424

0.377

Multiple sequence alignment