Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GM698_RS00795 Genome accession   NZ_CP046532
Coordinates   168951..170351 (-) Length   466 a.a.
NCBI ID   WP_209289485.1    Uniprot ID   -
Organism   Mannheimia sp. ZY171111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 163951..175351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM698_RS00765 (GM698_00765) lptE 164726..165214 (+) 489 WP_176808298.1 LPS assembly lipoprotein LptE -
  GM698_RS00770 (GM698_00770) holA 165255..166277 (+) 1023 WP_176808297.1 DNA polymerase III subunit delta -
  GM698_RS00775 (GM698_00775) yacG 166278..166463 (-) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  GM698_RS00780 (GM698_00780) coaE 166445..167083 (-) 639 WP_209289483.1 dephospho-CoA kinase -
  GM698_RS00785 (GM698_00785) - 167115..167771 (-) 657 WP_209289484.1 prepilin peptidase -
  GM698_RS00790 (GM698_00790) pilC 167774..168967 (-) 1194 WP_176812460.1 type II secretion system F family protein Machinery gene
  GM698_RS00795 (GM698_00795) pilB 168951..170351 (-) 1401 WP_209289485.1 GspE/PulE family protein Machinery gene
  GM698_RS00800 (GM698_00800) pilA 170373..170816 (-) 444 WP_209289486.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GM698_RS00805 (GM698_00805) radA 170997..172376 (+) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  GM698_RS00810 (GM698_00810) - 172415..172648 (-) 234 WP_159629593.1 YgjV family protein -
  GM698_RS00815 (GM698_00815) - 172776..173270 (-) 495 WP_159629590.1 dihydrofolate reductase -
  GM698_RS00820 (GM698_00820) proB 173384..174475 (+) 1092 WP_209289487.1 glutamate 5-kinase -
  GM698_RS00825 (GM698_00825) - 174475..175239 (+) 765 WP_176812456.1 TSUP family transporter -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 52745.53 Da        Isoelectric Point: 5.9944

>NTDB_id=405168 GM698_RS00795 WP_209289485.1 168951..170351(-) (pilB) [Mannheimia sp. ZY171111]
MAYSVTDLNSQQIIEISAEQWAKNGNEKQILLRYLAVPLRETESQLWLAIDDMQNLSACEIFAFIHHKQIEPVLISSDEL
KFLLNALSPEQSRLQSEIYEDNAAYSLSNAEQVDRNDPIIQILDNLFKFCLKNNASDIHLEPQKERLLIRLRIDGVLHLY
KTFPQNVSNRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRIQKNKPINLDFLELGFTP
SQKEKLLNALNQPQGLILVTGPTGSGKSITLYSALSHLNSNEKHILTAEDPIEIEIEGIIQTQVNRGIHLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNALLLVVAQRLLRRTCTKCGGI
GCENCYQGYKGRIGIYQLLSRTAKNFEKTTACLDFETLAESAKEKLVEGKTDRNEILRVLGNGENL

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=405168 GM698_RS00795 WP_209289485.1 168951..170351(-) (pilB) [Mannheimia sp. ZY171111]
ATGGCGTATTCAGTTACCGATCTTAACAGCCAGCAAATCATTGAAATTTCTGCCGAACAATGGGCAAAAAACGGCAATGA
AAAGCAGATTTTGTTGCGATATTTAGCCGTTCCGTTGCGAGAAACTGAATCACAATTATGGTTAGCGATTGATGATATGC
AAAACTTGAGTGCGTGTGAAATCTTTGCTTTTATTCATCATAAGCAGATTGAGCCTGTTTTGATTTCCTCTGATGAGTTG
AAATTTTTACTTAATGCCCTTTCACCCGAGCAGAGTCGCTTACAGAGTGAAATTTATGAGGATAATGCCGCTTATTCGTT
ATCAAATGCAGAGCAGGTAGATAGAAACGATCCGATTATTCAAATTTTAGATAATCTCTTTAAATTTTGCTTAAAAAATA
ATGCCTCAGACATCCATTTGGAGCCTCAGAAAGAGAGGTTACTCATTCGACTAAGAATTGATGGAGTTTTACATCTTTAT
AAAACGTTTCCTCAGAATGTATCAAACCGACTGATTTCTCGAATTAAATTACTAGCGAAACTAGATATTAGTGAAACCCG
TTTGCCTCAAGATGGGCAATTTCATTTTAAAACGATTTTAGCAGAAACCTTAGATTTCCGCGTTTCAACTTTGCCTACGC
ATTTTGGTGAAAAAGTGGTACTTCGGATTCAAAAAAATAAGCCAATTAATTTGGACTTTTTAGAGTTAGGATTTACCCCA
AGCCAGAAAGAAAAATTGCTCAATGCACTGAATCAACCGCAAGGCTTGATTCTAGTAACAGGGCCGACAGGTAGTGGTAA
AAGTATCACGCTTTATAGTGCATTAAGTCATTTAAATAGCAATGAAAAACATATTTTAACTGCGGAAGATCCAATTGAAA
TTGAGATAGAGGGTATTATTCAAACTCAGGTAAATCGTGGTATTCATCTTGATTTTAGCCAACTACTGAGAACCTTCCTT
CGACAAGATCCTGACATCATTATGCTAGGTGAGATCCGTGATGAGGAGAGTGCAGAAATTGCCTTAAGAGCGGCACAAAC
TGGGCATTTGGTTCTTTCGACATTACATACTAATGATGCACCCTCTGCCATAGAGCGATTATTACAACTCGGGATAAAAG
AATACGAGCTGAAAAATGCGTTGTTGCTTGTGGTGGCTCAACGTTTATTAAGGCGAACTTGCACAAAATGTGGCGGTATC
GGTTGCGAAAATTGCTATCAAGGCTACAAAGGTAGAATTGGGATTTATCAACTCTTGAGCAGAACTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGATTTTGAAACCTTAGCTGAGAGTGCAAAAGAAAAATTAGTGGAAGGAAAAACAGATCGGA
ATGAAATTTTGAGGGTATTAGGAAATGGCGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.435

98.712

0.695
  pilB Haemophilus influenzae 86-028NP

57.269

97.425

0.558
  pilB Haemophilus influenzae Rd KW20

56.828

97.425

0.554
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.956

100

0.423
  pilB Vibrio cholerae strain A1552

39.834

100

0.412
  pilB Legionella pneumophila strain ERS1305867

39.22

100

0.41
  pilB Acinetobacter baylyi ADP1

37.295

100

0.391
  pilB Acinetobacter baumannii D1279779

36.81

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.712

100

0.382
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.446

89.485

0.38
  pilF Thermus thermophilus HB27

35.805

100

0.363

Multiple sequence alignment