Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GM698_RS00805 Genome accession   NZ_CP046532
Coordinates   170997..172376 (+) Length   459 a.a.
NCBI ID   WP_176808292.1    Uniprot ID   -
Organism   Mannheimia sp. ZY171111     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 165997..177376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM698_RS00775 (GM698_00775) yacG 166278..166463 (-) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  GM698_RS00780 (GM698_00780) coaE 166445..167083 (-) 639 WP_209289483.1 dephospho-CoA kinase -
  GM698_RS00785 (GM698_00785) - 167115..167771 (-) 657 WP_209289484.1 prepilin peptidase -
  GM698_RS00790 (GM698_00790) pilC 167774..168967 (-) 1194 WP_176812460.1 type II secretion system F family protein Machinery gene
  GM698_RS00795 (GM698_00795) pilB 168951..170351 (-) 1401 WP_209289485.1 GspE/PulE family protein Machinery gene
  GM698_RS00800 (GM698_00800) pilA 170373..170816 (-) 444 WP_209289486.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GM698_RS00805 (GM698_00805) radA 170997..172376 (+) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  GM698_RS00810 (GM698_00810) - 172415..172648 (-) 234 WP_159629593.1 YgjV family protein -
  GM698_RS00815 (GM698_00815) - 172776..173270 (-) 495 WP_159629590.1 dihydrofolate reductase -
  GM698_RS00820 (GM698_00820) proB 173384..174475 (+) 1092 WP_209289487.1 glutamate 5-kinase -
  GM698_RS00825 (GM698_00825) - 174475..175239 (+) 765 WP_176812456.1 TSUP family transporter -
  GM698_RS00830 (GM698_00830) nth 175242..175877 (+) 636 WP_176808290.1 endonuclease III -
  GM698_RS00835 (GM698_00835) - 175910..177148 (+) 1239 WP_209289488.1 multifunctional CCA addition/repair protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49817.36 Da        Isoelectric Point: 7.9649

>NTDB_id=405170 GM698_RS00805 WP_176808292.1 170997..172376(+) (radA) [Mannheimia sp. ZY171111]
MAKAPKTAYVCNDCGAEYSRWMGQCRECKAWNTISEVRLISSKESAKSDRFSGYAGETSGKVQALSDISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLAQNIPTLYVTGEESLQQVAMRANRLGLPAQNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGESDSRFRTLRSHKNRFGAVNELGVFAMTEQGLKEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALMSSFRNRPLPQDLVIFGEVG
LAGEIRPVTSGQERISEAAKHGFRRAIIPHGNAPKKPIKDMDVFTVKKLSDALDILYNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=405170 GM698_RS00805 WP_176808292.1 170997..172376(+) (radA) [Mannheimia sp. ZY171111]
ATGGCAAAAGCACCTAAAACCGCCTATGTTTGTAATGATTGTGGGGCGGAATACTCCCGTTGGATGGGGCAATGCCGAGA
ATGTAAGGCTTGGAATACCATCAGCGAAGTTAGGTTGATTTCGAGTAAAGAGTCAGCAAAATCTGACCGCTTTAGTGGTT
ACGCAGGTGAAACATCAGGCAAAGTGCAAGCACTTTCAGATATTAGCCTGCAAGAAGTGCCTCGCTTTAGCAGTGGTTTT
AATGAGCTAGATCGTGTGCTAGGTGGGGGGGTTGTACCAGGTTCTGCCATTCTTATTGGCGGACACCCTGGTGCAGGCAA
AAGTACGCTTTTACTCCAAGTAATGTGTGGCTTAGCTCAAAATATCCCTACTCTTTATGTTACAGGTGAGGAATCTTTGC
AGCAAGTGGCAATGCGTGCTAACCGTTTAGGTTTACCTGCACAAAATCTTAAAATGCTATCTGAAACCTCCGTTGAGCAT
ATTTGCAATATTGCCGATCAAGAAAAACCTAAAATTATGGTAATCGATTCTATTCAAGTGATGCACTTATCCGATATTCA
ATCCTCTCCAGGAAGCGTTTCACAGGTTCGAGAATGTGCTTCATTTTTAACTCGTTATGCTAAAACCCGCCAAGTCGCCA
TTATTATGGTAGGGCACGTTACTAAAGATGGGACACTTGCTGGCCCAAAAGTATTGGAACACGCTATTGATGCTTCATTA
TTACTTGAAGGGGAGTCCGATTCTCGATTCCGCACTTTGCGTAGCCATAAAAATCGTTTTGGTGCAGTAAATGAGTTAGG
TGTATTCGCAATGACTGAGCAGGGCTTGAAAGAAGTTAAAAATCCCTCTGCAATTTTCCTTAGCCGTAGCGAAGAACAAA
CGCCTGGTAGCTCTGTAATGGTGTTGTGGGAAGGAACTCGCCCGCTTCTAGTTGAAATTCAGGCTCTAGTCGATCATTCA
ATGCTAGCAAATCCTCGCCGTGTCGCCGTTGGTTTAGATCATAACCGTTTATCGCTACTGCTTGCGGTTTTACATCGACA
CGGCGGACTACAAATGTCAGATCAAGACGTGTTTGTCAACGTCGTAGGCGGTGTGAAAGTAACCGAAACCAGTGCCGATC
TGGCTCTGATCTTGGCTTTAATGTCTAGCTTTCGCAATCGCCCATTACCACAAGATTTAGTTATTTTTGGCGAGGTGGGA
TTAGCAGGGGAAATCCGTCCTGTGACTAGCGGACAAGAACGAATTAGTGAAGCAGCAAAACACGGCTTTAGGCGAGCCAT
CATTCCCCACGGTAATGCGCCTAAAAAGCCGATTAAAGATATGGACGTTTTTACTGTAAAAAAACTAAGTGATGCGTTAG
ATATTCTTTATAATTTATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(84-224)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

43.833

98.911

0.434
  radA Streptococcus mitis SK321

43.612

98.911

0.431
  radA Streptococcus pneumoniae Rx1

42.919

100

0.429
  radA Streptococcus pneumoniae D39

42.919

100

0.429
  radA Streptococcus pneumoniae R6

42.919

100

0.429
  radA Streptococcus pneumoniae TIGR4

42.919

100

0.429

Multiple sequence alignment