Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GM698_RS00800 Genome accession   NZ_CP046532
Coordinates   170373..170816 (-) Length   147 a.a.
NCBI ID   WP_209289486.1    Uniprot ID   -
Organism   Mannheimia sp. ZY171111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 165373..175816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM698_RS00775 (GM698_00775) yacG 166278..166463 (-) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  GM698_RS00780 (GM698_00780) coaE 166445..167083 (-) 639 WP_209289483.1 dephospho-CoA kinase -
  GM698_RS00785 (GM698_00785) - 167115..167771 (-) 657 WP_209289484.1 prepilin peptidase -
  GM698_RS00790 (GM698_00790) pilC 167774..168967 (-) 1194 WP_176812460.1 type II secretion system F family protein Machinery gene
  GM698_RS00795 (GM698_00795) pilB 168951..170351 (-) 1401 WP_209289485.1 GspE/PulE family protein Machinery gene
  GM698_RS00800 (GM698_00800) pilA 170373..170816 (-) 444 WP_209289486.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GM698_RS00805 (GM698_00805) radA 170997..172376 (+) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  GM698_RS00810 (GM698_00810) - 172415..172648 (-) 234 WP_159629593.1 YgjV family protein -
  GM698_RS00815 (GM698_00815) - 172776..173270 (-) 495 WP_159629590.1 dihydrofolate reductase -
  GM698_RS00820 (GM698_00820) proB 173384..174475 (+) 1092 WP_209289487.1 glutamate 5-kinase -
  GM698_RS00825 (GM698_00825) - 174475..175239 (+) 765 WP_176812456.1 TSUP family transporter -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15901.43 Da        Isoelectric Point: 9.8339

>NTDB_id=405169 GM698_RS00800 WP_209289486.1 170373..170816(-) (pilA) [Mannheimia sp. ZY171111]
MQKIYLFRPLAKAFTLIELMIVIAIIAILATIAIPSYNAYTQKAALSELLRASASYKSDVELCIYQNNGPKDCSGGKNGI
QADKANSNKAKYLNTIKVNKGVITVTGKGSLNNYSYTLTPAFSNNTISWTVSCKGKDTSLFPAGFCN

Nucleotide


Download         Length: 444 bp        

>NTDB_id=405169 GM698_RS00800 WP_209289486.1 170373..170816(-) (pilA) [Mannheimia sp. ZY171111]
ATGCAAAAAATTTATCTTTTTCGACCGCTTGCAAAAGCGTTTACCTTGATTGAGCTAATGATCGTGATTGCAATTATTGC
CATTCTAGCCACTATTGCGATTCCATCTTACAACGCTTACACACAAAAAGCGGCTTTATCTGAATTATTGAGAGCATCGG
CTTCTTATAAATCAGATGTTGAGCTGTGTATTTATCAAAATAATGGACCTAAAGATTGTTCTGGTGGAAAAAACGGTATT
CAAGCCGATAAGGCGAATAGTAATAAAGCCAAATATCTTAATACGATTAAGGTAAATAAAGGCGTGATTACCGTAACAGG
AAAAGGCTCGCTTAATAATTATAGCTACACGCTAACACCAGCCTTTTCCAATAATACGATCAGTTGGACTGTAAGTTGTA
AGGGTAAAGATACGAGTCTGTTCCCCGCAGGTTTTTGTAATTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

70.47

100

0.714
  pilA Haemophilus influenzae 86-028NP

56.552

98.639

0.558
  pilA Haemophilus influenzae Rd KW20

55.862

98.639

0.551

Multiple sequence alignment