Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GM160_RS01520 Genome accession   NZ_CP046415
Coordinates   326011..327243 (-) Length   410 a.a.
NCBI ID   WP_156227605.1    Uniprot ID   A0A6I6D8E0
Organism   Guyparkeria halophila strain sp2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321011..332243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM160_RS01490 (GM160_01490) trmB 321085..321786 (-) 702 WP_136866755.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  GM160_RS01495 (GM160_01495) - 321791..322603 (-) 813 WP_136866754.1 thiazole synthase -
  GM160_RS01500 (GM160_01500) - 322639..323697 (-) 1059 WP_156227597.1 YgfZ/GcvT domain-containing protein -
  GM160_RS01505 (GM160_01505) - 323779..324240 (+) 462 WP_156227599.1 hypothetical protein -
  GM160_RS01510 (GM160_01510) coaE 324347..325021 (-) 675 WP_156227601.1 dephospho-CoA kinase -
  GM160_RS01515 (GM160_01515) pilD 325029..325922 (-) 894 WP_156227603.1 prepilin peptidase Machinery gene
  GM160_RS01520 (GM160_01520) pilC 326011..327243 (-) 1233 WP_156227605.1 type II secretion system F family protein Machinery gene
  GM160_RS01525 (GM160_01525) pilB 327272..328969 (-) 1698 WP_156227607.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GM160_RS01530 (GM160_01530) - 329090..329533 (-) 444 WP_156227609.1 PAS domain-containing protein -
  GM160_RS01535 (GM160_01535) - 329625..330599 (-) 975 WP_231505793.1 MerR family transcriptional regulator -
  GM160_RS01540 (GM160_01540) - 330745..331521 (+) 777 WP_136866747.1 SDR family NAD(P)-dependent oxidoreductase -
  GM160_RS01545 (GM160_01545) - 331518..331979 (+) 462 WP_156227612.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45349.55 Da        Isoelectric Point: 9.8018

>NTDB_id=404170 GM160_RS01520 WP_156227605.1 326011..327243(-) (pilC) [Guyparkeria halophila strain sp2]
MAIAASKNKTSEKPVFTYEARTKEGRVIKGEMAAFNDTVVRAEIRRLGMTLIRVKRKAKPLFSSSKPVQPRDIATFARQL
ATMMTAGLPIVQALDIIAQSAEKKTMKDLINEVKNDVESGTNLADALDRHRKYFDELFVNLVRAGEQSGALEVVLDRIAT
YKEKTESLKGKIKKALFYPTAVLVVAFIVTAILLIFVIPQFESLFQGFGADLPAFTQFVIGLSDLFREYWYWIFGGIAAV
IFLFVFVKRRSDRFNHFLDRMILKLPIFGPLMEKSAIARFARTLSTMFSAGVPLVEALPPTAGATGNAVFRDAVMSMRDL
VSAGSPLRIGMQQSNLFPVMVIQMVAIGEETGALDNMLAKVADMYEEEVDNMVDAMSSLLEPMIMAFLGIVVGGLVIAMY
LPIFKLGAVV

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=404170 GM160_RS01520 WP_156227605.1 326011..327243(-) (pilC) [Guyparkeria halophila strain sp2]
ATGGCCATTGCGGCAAGCAAGAACAAGACAAGCGAAAAACCCGTCTTCACCTACGAGGCGCGCACCAAGGAAGGCCGGGT
GATCAAGGGCGAGATGGCGGCGTTCAACGACACCGTCGTGCGTGCCGAGATCCGGCGTCTGGGCATGACCCTGATCCGGG
TCAAGCGCAAGGCCAAGCCGCTTTTCTCCAGCTCCAAGCCCGTCCAGCCGCGCGACATCGCCACCTTCGCCCGGCAGCTC
GCCACCATGATGACCGCCGGCCTGCCCATCGTGCAGGCCTTGGACATCATCGCCCAGAGCGCCGAGAAGAAGACGATGAA
GGACCTCATCAACGAGGTGAAGAACGACGTCGAGAGCGGCACCAACCTCGCCGACGCCCTCGATCGGCACCGCAAGTACT
TCGACGAGCTGTTCGTCAATCTCGTGCGCGCCGGCGAGCAGTCCGGTGCCCTCGAGGTGGTGCTCGACCGCATCGCGACC
TACAAGGAAAAGACCGAGAGCCTCAAGGGCAAGATCAAGAAGGCACTGTTCTATCCGACCGCCGTACTCGTGGTCGCCTT
CATCGTTACCGCGATCCTGCTGATCTTCGTTATCCCGCAGTTCGAGTCGCTATTCCAGGGCTTCGGCGCCGACCTGCCGG
CCTTCACCCAGTTCGTCATCGGCCTGTCCGACCTGTTCCGCGAATACTGGTACTGGATCTTCGGCGGCATCGCCGCGGTG
ATCTTCCTGTTCGTGTTCGTCAAGCGTCGCTCGGATCGCTTCAACCATTTCCTCGACCGGATGATCCTCAAGCTGCCGAT
CTTTGGCCCACTGATGGAGAAATCGGCGATCGCGCGTTTCGCCCGCACCCTGTCGACCATGTTCTCCGCCGGCGTGCCGC
TGGTCGAGGCCCTGCCGCCGACCGCCGGGGCAACCGGCAACGCCGTGTTCCGCGACGCGGTGATGAGCATGCGCGACCTG
GTCTCCGCGGGCTCGCCACTGCGCATCGGCATGCAGCAGTCCAACCTGTTCCCGGTGATGGTCATCCAGATGGTCGCCAT
CGGCGAGGAAACCGGCGCGCTCGACAACATGCTGGCCAAGGTGGCCGACATGTACGAAGAGGAGGTCGACAACATGGTCG
ATGCGATGTCGAGCCTGCTCGAGCCCATGATCATGGCCTTCCTCGGCATCGTGGTCGGCGGTCTCGTCATCGCCATGTAC
CTGCCGATCTTCAAGCTGGGCGCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6D8E0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

55.556

96.585

0.537
  pilC Acinetobacter baylyi ADP1

52.593

98.78

0.52
  pilC Pseudomonas stutzeri DSM 10701

51.861

98.293

0.51
  pilC Acinetobacter baumannii D1279779

52.13

97.317

0.507
  pilC Vibrio cholerae strain A1552

43.434

96.585

0.42
  pilC Vibrio campbellii strain DS40M4

42.857

97.317

0.417
  pilG Neisseria meningitidis 44/76-A

41.414

96.585

0.4
  pilG Neisseria gonorrhoeae MS11

41.162

96.585

0.398
  pilC Thermus thermophilus HB27

40.5

97.561

0.395

Multiple sequence alignment