Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GM160_RS01525 Genome accession   NZ_CP046415
Coordinates   327272..328969 (-) Length   565 a.a.
NCBI ID   WP_156227607.1    Uniprot ID   A0A6I6D1E1
Organism   Guyparkeria halophila strain sp2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322272..333969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM160_RS01500 (GM160_01500) - 322639..323697 (-) 1059 WP_156227597.1 YgfZ/GcvT domain-containing protein -
  GM160_RS01505 (GM160_01505) - 323779..324240 (+) 462 WP_156227599.1 hypothetical protein -
  GM160_RS01510 (GM160_01510) coaE 324347..325021 (-) 675 WP_156227601.1 dephospho-CoA kinase -
  GM160_RS01515 (GM160_01515) pilD 325029..325922 (-) 894 WP_156227603.1 prepilin peptidase Machinery gene
  GM160_RS01520 (GM160_01520) pilC 326011..327243 (-) 1233 WP_156227605.1 type II secretion system F family protein Machinery gene
  GM160_RS01525 (GM160_01525) pilB 327272..328969 (-) 1698 WP_156227607.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GM160_RS01530 (GM160_01530) - 329090..329533 (-) 444 WP_156227609.1 PAS domain-containing protein -
  GM160_RS01535 (GM160_01535) - 329625..330599 (-) 975 WP_231505793.1 MerR family transcriptional regulator -
  GM160_RS01540 (GM160_01540) - 330745..331521 (+) 777 WP_136866747.1 SDR family NAD(P)-dependent oxidoreductase -
  GM160_RS01545 (GM160_01545) - 331518..331979 (+) 462 WP_156227612.1 nuclear transport factor 2 family protein -
  GM160_RS01550 (GM160_01550) - 331939..333324 (-) 1386 WP_136866745.1 MFS transporter -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61370.11 Da        Isoelectric Point: 4.7332

>NTDB_id=404171 GM160_RS01525 WP_156227607.1 327272..328969(-) (pilB) [Guyparkeria halophila strain sp2]
MSAPPVNGLLSALVREEALSSDQAKTIQSKAEASEDSLLNTVIAEGVSAATIAAVASKRFGLPLVDLDAIDTSDLPLKLV
PEKVLQANMALPLFKRGRRLTVAVADPSDTGAIDGFRFSTNMAIEAVVAEAPKIARLLEEALSDSMDALGDELDDDLSNV
EFEREDDEAHDVEDASIQDDVVVKFVTKLLTDAIRKGASDIHIEPYEKQFRVRFRIDGMLTESAKPPANLADRIIARVKV
MSRMDISERRIPQDGRIKLKLSKNRSIDFRVNTCPTLFGEKVVLRILDPTSAQLGIDQLGYEPDQKALYMDALDKPYGMI
LVTGPTGSGKTVSLYTGLNILNSGDRNISTAEDPAEIVVPGINQVNVNPKVGLSFANALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNDAPQTLTRMVNMGVPAYNIASSVSLIIAQRLARRLCNNCRTKESIPDEELRREGFTAED
LAGAPTIYKAVGCDQCTNGYKGRVGIYQVMPVSEAMGRIIMEGGNAMDIADQARKEGIDDLRRSGLKKVLAGVTSLEEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=404171 GM160_RS01525 WP_156227607.1 327272..328969(-) (pilB) [Guyparkeria halophila strain sp2]
ATGAGCGCACCCCCCGTCAATGGACTGCTCAGCGCCCTGGTCAGGGAGGAAGCCCTGTCCTCCGACCAGGCAAAGACCAT
TCAATCCAAGGCCGAAGCCAGCGAGGACTCGCTGCTCAACACCGTGATCGCGGAGGGCGTGTCGGCGGCGACCATCGCCG
CCGTCGCCTCCAAGCGTTTCGGCCTGCCGCTGGTGGACCTCGATGCCATCGACACCAGCGACCTGCCCCTGAAGCTCGTT
CCCGAGAAGGTCCTGCAGGCCAACATGGCGCTGCCGCTGTTCAAGCGCGGACGTCGCCTGACGGTGGCCGTGGCCGACCC
CTCCGACACCGGCGCGATCGACGGCTTCCGCTTCTCGACCAACATGGCGATCGAGGCGGTGGTCGCCGAGGCGCCCAAGA
TCGCGCGCCTGCTCGAGGAAGCCCTGAGCGACAGCATGGACGCCCTGGGCGACGAGCTTGACGACGACCTGTCGAACGTC
GAGTTCGAGCGCGAGGACGACGAGGCCCACGACGTCGAGGATGCCTCGATCCAGGACGACGTGGTGGTCAAGTTCGTCAC
CAAGCTCCTGACCGACGCGATCCGCAAGGGCGCCTCGGACATCCACATCGAGCCCTACGAGAAGCAGTTCCGCGTGCGCT
TTCGCATCGACGGCATGCTCACCGAGAGCGCCAAGCCGCCGGCCAACCTGGCCGACCGCATCATCGCGCGCGTCAAGGTG
ATGAGCCGCATGGACATCTCCGAGCGGCGCATCCCGCAGGACGGGCGCATCAAGCTCAAGCTGTCGAAGAACCGCTCGAT
CGACTTCCGCGTGAACACCTGCCCGACCCTGTTCGGCGAGAAGGTCGTGCTGCGTATCCTCGACCCGACCAGCGCGCAGC
TGGGCATCGACCAGCTCGGCTACGAGCCCGATCAGAAAGCGCTCTACATGGACGCGCTCGACAAGCCCTACGGCATGATC
CTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTCTACACCGGCCTGAACATCCTCAACTCGGGCGATCGCAA
CATCTCGACCGCCGAGGACCCGGCGGAAATCGTCGTACCGGGCATCAACCAGGTCAACGTCAACCCCAAGGTGGGCCTGT
CGTTCGCCAATGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTGGGCGAGATCCGGGATCTGGAAACC
GCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACGACGCGCCGCAGACGCT
CACGCGGATGGTGAACATGGGCGTGCCGGCCTACAACATCGCCTCCTCGGTGAGCCTGATCATCGCGCAGCGACTGGCGC
GGCGCCTGTGCAATAATTGCCGCACGAAGGAGAGCATCCCCGACGAGGAACTGCGCCGCGAGGGGTTCACCGCCGAGGAT
CTCGCGGGTGCGCCGACCATCTACAAGGCGGTGGGCTGCGATCAGTGCACCAACGGCTACAAGGGCCGGGTCGGCATCTA
TCAGGTCATGCCCGTGTCCGAGGCGATGGGGCGGATCATCATGGAAGGCGGCAACGCCATGGACATCGCCGACCAGGCGC
GTAAGGAAGGCATCGACGACCTGCGACGCTCCGGTCTGAAGAAGGTGCTCGCCGGCGTGACCAGCCTCGAGGAAATCAAC
CGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6D1E1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.14

100

0.566
  pilB Acinetobacter baylyi ADP1

55.124

100

0.552
  pilB Legionella pneumophila strain ERS1305867

53.616

100

0.538
  pilF Neisseria gonorrhoeae MS11

50.355

99.823

0.503
  pilB Vibrio cholerae strain A1552

48.057

100

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.05

99.823

0.48
  pilB Vibrio campbellii strain DS40M4

47.95

99.292

0.476
  pilF Thermus thermophilus HB27

38.649

94.336

0.365

Multiple sequence alignment