Detailed information NTG0404169

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GM160_RS01515
• Genome accession: NZ_CP046415
• Coordinates: 325029..325922 (-)
• Length: 297 a.a.
• NCBI ID: WP_156227603.1
• Uniprot ID: A0A6I6D2W4
• Organism: Guyparkeria halophila strain sp2
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 320029..330922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GM160_RS01485 (GM160_01485)	-	320800..321078 (-)	279	WP_136866756.1	YrhK family protein	-
GM160_RS01490 (GM160_01490)	trmB	321085..321786 (-)	702	WP_136866755.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
GM160_RS01495 (GM160_01495)	-	321791..322603 (-)	813	WP_136866754.1	thiazole synthase	-
GM160_RS01500 (GM160_01500)	-	322639..323697 (-)	1059	WP_156227597.1	YgfZ/GcvT domain-containing protein	-
GM160_RS01505 (GM160_01505)	-	323779..324240 (+)	462	WP_156227599.1	hypothetical protein	-
GM160_RS01510 (GM160_01510)	coaE	324347..325021 (-)	675	WP_156227601.1	dephospho-CoA kinase	-
GM160_RS01515 (GM160_01515)	pilD	325029..325922 (-)	894	WP_156227603.1	prepilin peptidase	Machinery gene
GM160_RS01520 (GM160_01520)	pilC	326011..327243 (-)	1233	WP_156227605.1	type II secretion system F family protein	Machinery gene
GM160_RS01525 (GM160_01525)	pilB	327272..328969 (-)	1698	WP_156227607.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GM160_RS01530 (GM160_01530)	-	329090..329533 (-)	444	WP_156227609.1	PAS domain-containing protein	-
GM160_RS01535 (GM160_01535)	-	329625..330599 (-)	975	WP_231505793.1	MerR family transcriptional regulator	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 32846.13 Da
• Isoelectric Point: 6.4749

>NTDB_id=404169 GM160_RS01515 WP_156227603.1 325029..325922(-) (pilD) [Guyparkeria halophila strain sp2]
MTELQAIFQQHPAIWIIVASLFGLLVGSFLNVVINRLPVMMEREWKRECRALLADEGSETQVPEEEGEPFNLVQPRSRCP
SCGTPIRAWQNIPVISWLWLRGRCAKCRAPIAWQYPAVELAGALLAGLAAWHFGPTALALAVFLFSWALLAASIIDLKTQ
LLPDVFTLPLLWLGLLLPIVLDDYHLSLDQAVLGAVFGYLALWSVYWLFRLLTGKEGMGFGDFKLLAALGAWLGWQMLPL
VILFSALAGSVIGIAMILLLRHDRRIPIPFGPYLAIAGLVALYLGEPIMTAYLGGPF

Nucleotide

• Length: 894 bp

>NTDB_id=404169 GM160_RS01515 WP_156227603.1 325029..325922(-) (pilD) [Guyparkeria halophila strain sp2]
ATGACCGAACTGCAAGCCATCTTCCAGCAACATCCGGCCATCTGGATCATTGTGGCCAGCCTGTTCGGCCTGCTGGTGGG
CAGCTTTCTCAATGTCGTCATCAACCGGCTGCCGGTAATGATGGAGCGCGAGTGGAAGCGCGAATGCCGCGCCCTGCTGG
CCGACGAGGGCAGCGAGACGCAGGTGCCCGAGGAGGAGGGGGAGCCGTTCAACCTGGTCCAGCCGCGCTCGCGCTGCCCG
TCCTGCGGCACCCCGATTCGGGCGTGGCAGAACATCCCGGTGATCAGTTGGCTTTGGCTGCGCGGGCGCTGCGCCAAGTG
CCGCGCGCCGATCGCCTGGCAGTACCCGGCCGTGGAGCTAGCCGGCGCACTGCTCGCCGGCCTGGCTGCCTGGCATTTCG
GCCCGACCGCGCTGGCACTGGCCGTTTTCCTGTTCAGCTGGGCCCTGCTGGCCGCCTCGATCATCGACCTCAAGACCCAA
CTGCTGCCCGACGTGTTCACCCTGCCGCTCTTGTGGCTGGGCCTGCTCCTGCCGATCGTGCTGGACGACTACCATCTCTC
GCTGGACCAGGCAGTGCTGGGCGCCGTGTTCGGCTACCTCGCGTTATGGTCGGTGTACTGGTTGTTCCGGCTGCTCACCG
GCAAGGAAGGCATGGGCTTCGGCGACTTCAAGTTGCTCGCCGCGCTGGGGGCCTGGCTGGGCTGGCAGATGCTGCCGCTG
GTGATCCTGTTCTCGGCCCTGGCCGGCAGCGTGATCGGCATCGCCATGATCCTCTTGCTGCGCCACGACCGGCGCATCCC
CATCCCCTTCGGCCCCTATCTGGCGATCGCAGGGCTGGTCGCCCTGTATCTGGGCGAGCCGATCATGACGGCCTATCTGG
GCGGTCCGTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (144-254)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-133)

3D structure

• AlphaFold DB: A0A6I6D2W4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.916	96.633	0.502
pilD	Vibrio cholerae strain A1552	51.568	96.633	0.498
pilD	Acinetobacter baumannii D1279779	50.169	99.327	0.498
pilD	Acinetobacter nosocomialis M2	49.831	99.327	0.495
pilD	Neisseria gonorrhoeae MS11	46.494	91.246	0.424