Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB   Type   Machinery gene
Locus tag   GMB29_RS25465 Genome accession   NZ_CP046266
Coordinates   5301137..5301484 (-) Length   115 a.a.
NCBI ID   WP_136357245.1    Uniprot ID   A0A4S4BRI4
Organism   Metabacillus sediminilitoris strain DSL-17     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 5296137..5306484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMB29_RS25450 (GMB29_25450) - 5297524..5299020 (-) 1497 WP_136357095.1 MFS transporter -
  GMB29_RS25455 (GMB29_25455) - 5299302..5299991 (-) 690 WP_136357097.1 DUF4867 family protein -
  GMB29_RS25460 (GMB29_25460) - 5300088..5300768 (-) 681 WP_136357099.1 transaldolase family protein -
  GMB29_RS25465 (GMB29_25465) ssbB 5301137..5301484 (-) 348 WP_136357245.1 single-stranded DNA-binding protein Machinery gene
  GMB29_RS27540 - 5302042..5302252 (+) 211 Protein_5021 hypothetical protein -
  GMB29_RS25470 (GMB29_25470) - 5302391..5302837 (+) 447 WP_227551431.1 YwpF-like family protein -
  GMB29_RS25475 (GMB29_25475) - 5303032..5303601 (-) 570 WP_136357101.1 manganese catalase family protein -
  GMB29_RS25480 (GMB29_25480) - 5303770..5304030 (-) 261 WP_136357103.1 spore coat protein CotJB -
  GMB29_RS25485 (GMB29_25485) - 5304033..5304281 (-) 249 WP_136357248.1 spore coat associated protein CotJA -
  GMB29_RS25490 (GMB29_25490) - 5304635..5304961 (-) 327 WP_136357105.1 hypothetical protein -
  GMB29_RS25495 (GMB29_25495) fabZ 5305137..5305577 (-) 441 WP_136357107.1 3-hydroxyacyl-ACP dehydratase FabZ -
  GMB29_RS25500 (GMB29_25500) - 5305697..5305966 (-) 270 WP_227551432.1 DNA-directed RNA polymerase subunit beta -

Sequence

Protein


Download         Length: 115 a.a.        Molecular weight: 12897.58 Da        Isoelectric Point: 8.4857

>NTDB_id=402111 GMB29_RS25465 WP_136357245.1 5301137..5301484(-) (ssbB) [Metabacillus sediminilitoris strain DSL-17]
MINQVILVGRLTKDPEIRYTADGAPVANITLAVSRQFRNAAGEIDTDFVNCTVWRRTAENTANYCRKGSIVGVTGRIQTR
SYENAERVRVYVTEVVADSVRFMSGKPRELVEQES

Nucleotide


Download         Length: 348 bp        

>NTDB_id=402111 GMB29_RS25465 WP_136357245.1 5301137..5301484(-) (ssbB) [Metabacillus sediminilitoris strain DSL-17]
GTGATCAATCAAGTCATTTTAGTAGGCAGGTTAACGAAAGACCCAGAAATCCGCTATACAGCAGACGGTGCTCCTGTAGC
TAATATTACCCTTGCTGTAAGTCGTCAATTTCGCAATGCCGCAGGAGAAATTGACACAGATTTCGTAAACTGCACGGTCT
GGAGACGAACAGCAGAGAACACGGCAAATTACTGCCGAAAAGGCTCAATCGTCGGTGTCACAGGTAGAATCCAGACACGA
AGTTATGAAAATGCAGAGCGCGTCCGTGTCTATGTCACCGAAGTTGTAGCTGACTCCGTTCGTTTCATGAGCGGAAAACC
ACGAGAGTTAGTTGAGCAAGAAAGTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4S4BRI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Bacillus subtilis subsp. subtilis str. 168

73.394

94.783

0.696
  ssbA Bacillus subtilis subsp. subtilis str. 168

61.321

92.174

0.565
  ssb Latilactobacillus sakei subsp. sakei 23K

54.867

98.261

0.539
  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.593

93.913

0.4
  ssbA Streptococcus mutans UA159

40.741

93.913

0.383
  ssbB/cilA Streptococcus pneumoniae TIGR4

39.815

93.913

0.374
  ssbB/cilA Streptococcus pneumoniae Rx1

38.889

93.913

0.365
  ssbB/cilA Streptococcus pneumoniae D39

38.889

93.913

0.365
  ssbB/cilA Streptococcus pneumoniae R6

38.889

93.913

0.365
  ssbB/cilA Streptococcus mitis SK321

38.889

93.913

0.365
  ssb Vibrio cholerae strain A1552

42.857

85.217

0.365

Multiple sequence alignment