Detailed information NTG0401497

Bioinformatically predicted

Overview

• Name: ssbA
• Type: Machinery gene
• Locus tag: GKC13_RS00960
• Genome accession: NZ_CP046134
• Coordinates: 167350..167742 (+)
• Length: 130 a.a.
• NCBI ID: WP_011226610.1
• Uniprot ID: A0A3G6K2H6
• Organism: Streptococcus thermophilus strain MAG_rmk202_sterm
• Function: ssDNA binding (predicted from homology) DNA processing

Genomic Context

Location: 162350..172742

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC13_RS00925 (GKC13_00920)	proC	162546..163322 (-)	777	WP_207559850.1	pyrroline-5-carboxylate reductase	-
GKC13_RS00930 (GKC13_00925)	pepA	163338..164405 (-)	1068	WP_002952022.1	glutamyl aminopeptidase	-
GKC13_RS00935 (GKC13_00930)	-	164505..164789 (+)	285	WP_011681678.1	DUF4651 domain-containing protein	-
GKC13_RS00940 (GKC13_00935)	-	164786..165100 (+)	315	WP_002945230.1	thioredoxin family protein	-
GKC13_RS00945 (GKC13_00940)	ytpR	165110..165733 (+)	624	WP_002952017.1	YtpR family tRNA-binding protein	-
GKC13_RS00950 (GKC13_00945)	-	165871..166902 (+)	1032	Protein_182	YeeE/YedE family protein	-
GKC13_RS00955 (GKC13_00950)	-	166917..167144 (+)	228	WP_084829880.1	sulfurtransferase TusA family protein	-
GKC13_RS00960 (GKC13_00955)	ssbA	167350..167742 (+)	393	WP_011226610.1	single-stranded DNA-binding protein	Machinery gene
GKC13_RS00965 (GKC13_00960)	tadA	168503..169021 (+)	519	WP_179970809.1	tRNA adenosine(34) deaminase TadA	-
GKC13_RS00970 (GKC13_00965)	-	169102..169365 (-)	264	WP_011681674.1	hypothetical protein	-
GKC13_RS00975 (GKC13_00970)	-	169418..169585 (-)	168	WP_002945733.1	class IIb bacteriocin, lactobin A/cerein 7B family	-
GKC13_RS00985 (GKC13_00980)	-	169954..170787 (+)	834	WP_179970810.1	putative RNA methyltransferase	-
GKC13_RS10435 (GKC13_00985)	-	170902..171656 (+)	755	Protein_189	HAD family hydrolase	-
GKC13_RS00995 (GKC13_00990)	dapD	171742..172440 (+)	699	WP_002951996.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase	-

Sequence

Protein

• Length: 130 a.a.
• Molecular weight: 14773.70 Da
• Isoelectric Point: 5.9275

>NTDB_id=401497 GKC13_RS00960 WP_011226610.1 167350..167742(+) (ssbA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MYNKVILIGRLTATPEMVKTASDRSFTRVTVAVNRRYKTQNGEREADFITVVVWGRLAETLASYASKGSLISLDGEIRTR
KYDKDGQTHYVTEVLCSSFQLLESRAQRAVRENNPDNDLADLILEEELPF

Nucleotide

• Length: 393 bp

>NTDB_id=401497 GKC13_RS00960 WP_011226610.1 167350..167742(+) (ssbA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATGTATAACAAAGTAATTTTAATTGGACGTTTGACAGCAACACCTGAAATGGTTAAGACTGCTAGTGACAGGTCTTTTAC
ACGTGTCACAGTTGCGGTTAATCGTCGCTATAAGACGCAAAATGGTGAGCGTGAGGCTGATTTTATCACAGTAGTGGTTT
GGGGCCGTTTAGCTGAGACATTGGCATCTTATGCTAGCAAGGGAAGTTTAATTTCTCTTGATGGTGAGATTCGCACTCGT
AAGTATGATAAGGATGGTCAAACTCATTACGTTACTGAAGTGCTTTGTTCCTCTTTCCAACTTCTGGAGAGTCGTGCTCA
GCGTGCTGTTAGGGAAAATAATCCTGACAATGACTTGGCTGACCTGATTTTAGAAGAAGAGCTTCCCTTTTAG

Domains

Predicted by InterproScan.

• Single-strand binding protein family (SSB) (3-102)

3D structure

• AlphaFold DB: A0A3G6K2H6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ssbA	Streptococcus mutans UA159	79.389	100	0.8
ssbB	Streptococcus sobrinus strain NIDR 6715-7	76.336	100	0.769
ssbB/cilA	Streptococcus pneumoniae TIGR4	74.809	100	0.754
ssbB/cilA	Streptococcus mitis SK321	74.046	100	0.746
ssbB/cilA	Streptococcus pneumoniae R6	74.046	100	0.746
ssbB/cilA	Streptococcus pneumoniae Rx1	74.046	100	0.746
ssbB/cilA	Streptococcus pneumoniae D39	74.046	100	0.746
ssbB/cilA	Streptococcus mitis NCTC 12261	72.519	100	0.731
ssbB	Lactococcus lactis subsp. cremoris KW2	62.5	86.154	0.538
ssbA	Bacillus subtilis subsp. subtilis str. 168	47.17	81.538	0.385
ssb	Latilactobacillus sakei subsp. sakei 23K	46.226	81.538	0.377