Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   GJS33_RS00715 Genome accession   NZ_CP046041
Coordinates   126621..127559 (+) Length   312 a.a.
NCBI ID   WP_111692095.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain AZ-45470     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121621..132559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS33_RS00695 (GJS33_00695) - 121980..123212 (+) 1233 WP_014622136.1 hypothetical protein -
  GJS33_RS00700 (GJS33_00700) - 123532..123723 (+) 192 Protein_107 lactococcin 972 family bacteriocin -
  GJS33_RS00705 (GJS33_00705) - 123788..125791 (+) 2004 WP_111692093.1 DUF1430 domain-containing protein -
  GJS33_RS00710 (GJS33_00710) - 125793..126440 (+) 648 WP_111692094.1 ABC transporter ATP-binding protein -
  GJS33_RS00715 (GJS33_00715) comGA/cglA 126621..127559 (+) 939 WP_111692095.1 competence type IV pilus ATPase ComGA Machinery gene
  GJS33_RS00720 (GJS33_00720) comYB 127492..128526 (+) 1035 WP_228269172.1 competence type IV pilus assembly protein ComGB Machinery gene
  GJS33_RS00725 (GJS33_00725) comGC/cglC 128527..128850 (+) 324 WP_012514782.1 competence type IV pilus major pilin ComGC Machinery gene
  GJS33_RS00730 (GJS33_00730) comGD 128828..129253 (+) 426 WP_111692097.1 competence type IV pilus minor pilin ComGD -
  GJS33_RS00735 (GJS33_00735) comGE 129258..129500 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  GJS33_RS00740 (GJS33_00740) comYF 129487..129921 (+) 435 WP_037580631.1 competence type IV pilus minor pilin ComGF Machinery gene
  GJS33_RS00745 (GJS33_00745) comGG 129899..130261 (+) 363 WP_111692098.1 competence type IV pilus minor pilin ComGG -
  GJS33_RS00750 (GJS33_00750) - 130329..131671 (+) 1343 WP_195759329.1 IS3 family transposase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35268.49 Da        Isoelectric Point: 6.8988

>NTDB_id=400501 GJS33_RS00715 WP_111692095.1 126621..127559(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain AZ-45470]
MIQELAKRLIRQAEELQAQDIYILPRGTGYELLMRVGDDRRLIDVCESDRMANLISHFKFIAGMNVGEKRRCQLGACDYD
LDGAKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQASQWNQQIDQLLAEGHLNTRQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=400501 GJS33_RS00715 WP_111692095.1 126621..127559(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain AZ-45470]
ATGATTCAAGAATTAGCCAAGCGGCTTATCAGGCAGGCTGAAGAATTGCAGGCTCAGGATATTTATATACTACCAAGGGG
GACAGGCTATGAGCTTTTGATGAGGGTAGGTGATGACAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCATTGCAGGAATGAATGTGGGAGAAAAAAGACGCTGCCAGCTCGGTGCTTGTGACTACGAC
CTTGATGGCGCTAAGGTATCTCTACGATTATCAGCTGTGGGTGACTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCCAGAGGCCTTT
ACCTCTTTTCTGGACCGGTTGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATTAAAAATGATCAGCTCCTGCAATTACAGGTTAACGACAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTTTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATCAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTAACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGCGTTTAC
GCTAGGCTGCTAGAATTAGGCATTTCAAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCGCAAAAGGAATTTGAACATCATCAAGCCAGCCAGTGGAATCAGCAGATTGATCAGC
TTCTTGCGGAGGGACATCTCAATACCAGGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

68.065

99.359

0.676
  comYA Streptococcus mutans UA140

62.701

99.679

0.625
  comYA Streptococcus mutans UA159

62.701

99.679

0.625
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

60.968

99.359

0.606
  comYA Streptococcus gordonii str. Challis substr. CH1

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae D39

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae R6

60.968

99.359

0.606
  comGA Lactococcus lactis subsp. cremoris KW2

52.733

99.679

0.526
  comGA Latilactobacillus sakei subsp. sakei 23K

39.796

94.231

0.375

Multiple sequence alignment