Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GIG89_RS00640 Genome accession   NZ_CP045924
Coordinates   133700..134644 (+) Length   314 a.a.
NCBI ID   WP_019291447.1    Uniprot ID   -
Organism   Lactococcus petauri strain CF11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 128700..139644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIG89_RS00625 - 129961..131562 (-) 1602 WP_153924348.1 GH25 family lysozyme -
  GIG89_RS00630 guaB 131731..133212 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  GIG89_RS00635 rpsU 133338..133514 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  GIG89_RS00640 comGA 133700..134644 (+) 945 WP_019291447.1 competence type IV pilus ATPase ComGA Machinery gene
  GIG89_RS00645 comGB 134583..135602 (+) 1020 WP_255566838.1 competence type IV pilus assembly protein ComGB Machinery gene
  GIG89_RS00650 comYC 135621..135926 (+) 306 WP_153924350.1 competence type IV pilus major pilin ComGC Machinery gene
  GIG89_RS00655 comGD 135901..136317 (+) 417 WP_019291446.1 competence type IV pilus minor pilin ComGD Machinery gene
  GIG89_RS00660 comGE 136349..136582 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  GIG89_RS00665 comGF 136563..137003 (+) 441 WP_019291444.1 competence type IV pilus minor pilin ComGF -
  GIG89_RS00670 - 137235..138080 (+) 846 WP_019291443.1 zinc ABC transporter substrate-binding protein -
  GIG89_RS00675 - 138151..139035 (-) 885 WP_019291442.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35713.01 Da        Isoelectric Point: 6.9700

>NTDB_id=399168 GIG89_RS00640 WP_019291447.1 133700..134644(+) (comGA) [Lactococcus petauri strain CF11]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVENEFLQLQVNKVIGNDYDSLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=399168 GIG89_RS00640 WP_019291447.1 133700..134644(+) (comGA) [Lactococcus petauri strain CF11]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAAATGAATTTTTGCAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TTCCTTGATTAAGCTTTCGCTGCGACATAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513

Multiple sequence alignment