Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   GIG89_RS00645 Genome accession   NZ_CP045924
Coordinates   134583..135602 (+) Length   339 a.a.
NCBI ID   WP_255566838.1    Uniprot ID   -
Organism   Lactococcus petauri strain CF11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 129583..140602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIG89_RS00625 - 129961..131562 (-) 1602 WP_153924348.1 GH25 family lysozyme -
  GIG89_RS00630 guaB 131731..133212 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  GIG89_RS00635 rpsU 133338..133514 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  GIG89_RS00640 comGA 133700..134644 (+) 945 WP_019291447.1 competence type IV pilus ATPase ComGA Machinery gene
  GIG89_RS00645 comGB 134583..135602 (+) 1020 WP_255566838.1 competence type IV pilus assembly protein ComGB Machinery gene
  GIG89_RS00650 comYC 135621..135926 (+) 306 WP_153924350.1 competence type IV pilus major pilin ComGC Machinery gene
  GIG89_RS00655 comGD 135901..136317 (+) 417 WP_019291446.1 competence type IV pilus minor pilin ComGD Machinery gene
  GIG89_RS00660 comGE 136349..136582 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  GIG89_RS00665 comGF 136563..137003 (+) 441 WP_019291444.1 competence type IV pilus minor pilin ComGF -
  GIG89_RS00670 - 137235..138080 (+) 846 WP_019291443.1 zinc ABC transporter substrate-binding protein -
  GIG89_RS00675 - 138151..139035 (-) 885 WP_019291442.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39480.16 Da        Isoelectric Point: 9.5027

>NTDB_id=399169 GIG89_RS00645 WP_255566838.1 134583..135602(+) (comGB) [Lactococcus petauri strain CF11]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLAEAYFISKMREGLLNGKSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRKKLVQVATYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTL
FLGWLFSLFVFFYLCRYYWKKKTALEALRLMVKIPFVGRFVQLYLTAYFAREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDSGQKFEDALRFYPIFTKELSLIVEYGELKSKLGKELMIFSEENWSLFFERMERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=399169 GIG89_RS00645 WP_255566838.1 134583..135602(+) (comGB) [Lactococcus petauri strain CF11]
ATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAAGCTCATTCAACTCATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTCTCTGAAATTATTGATTTCCTTGAACGATCGGGTCTTGCGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTTAATGGGAAAAGTTTCTCTGGTATTTTGGCGAAGCTAAATTTTTATGAGGATGTT
GTGACTCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTATACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAAAAAGCTTGTCCAAGTTGCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATTATGCTTG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCCTC
TTTTTAGGGTGGCTCTTCAGTTTGTTCGTTTTCTTTTATCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GCTGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTGCAAGAGAATGGG
GAAACTTAATTGCTCAAGCTGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATTTTCAAAGAA
TTCGGTTTTGAGCTTCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCTCTTCGATTTTACCCCATATTTACCAA
GGAGCTCTCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGATTTTTTCTGAAGAAAACT
GGTCGCTCTTTTTTGAAAGGATGGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCGGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.976

99.115

0.525
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.395

98.525

0.516
  comYB Streptococcus mutans UA140

52.548

92.625

0.487
  comYB Streptococcus mutans UA159

52.229

92.625

0.484

Multiple sequence alignment