Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GII23_RS04555 Genome accession   NZ_CP045858
Coordinates   993641..994786 (+) Length   381 a.a.
NCBI ID   WP_041108398.1    Uniprot ID   A0A8D3Y4G4
Organism   Stutzerimonas balearica strain EC28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 988641..999786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII23_RS04535 (GII23_04530) - 990322..990912 (-) 591 WP_041108406.1 YggT family protein -
  GII23_RS04540 (GII23_04535) proC 990922..991755 (-) 834 WP_165562574.1 pyrroline-5-carboxylate reductase -
  GII23_RS04545 (GII23_04540) - 991775..992464 (-) 690 WP_165562575.1 YggS family pyridoxal phosphate-dependent enzyme -
  GII23_RS04550 (GII23_04545) pilT 992530..993564 (+) 1035 WP_043222415.1 type IV pilus twitching motility protein PilT Machinery gene
  GII23_RS04555 (GII23_04550) pilU 993641..994786 (+) 1146 WP_041108398.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GII23_RS04560 (GII23_04555) - 994752..995636 (-) 885 WP_165562576.1 TIGR03915 family putative DNA repair protein -
  GII23_RS04565 (GII23_04560) - 995636..996847 (-) 1212 WP_043222410.1 putative DNA modification/repair radical SAM protein -
  GII23_RS04570 (GII23_04565) - 996944..997345 (+) 402 WP_041108393.1 TM2 domain-containing protein -
  GII23_RS04575 (GII23_04570) - 997383..998654 (-) 1272 WP_061339830.1 dihydroorotase -
  GII23_RS04580 (GII23_04575) - 998648..999670 (-) 1023 WP_041108389.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42517.82 Da        Isoelectric Point: 6.6647

>NTDB_id=398708 GII23_RS04555 WP_041108398.1 993641..994786(+) (pilU) [Stutzerimonas balearica strain EC28]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNTSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYVQGEITYEDALAHADSANDLRLMIKLGSETDSEHLTSVSQGLRLEVSDDDPGFRMR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=398708 GII23_RS04555 WP_041108398.1 993641..994786(+) (pilU) [Stutzerimonas balearica strain EC28]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAGAAGGGCGGCTCCGACCTGTTCATCACGGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACCGTGCATGGGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAGAACCACGAGTGCAATTTCGCCATCAGCGCTCGGGGGATCGGGCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAACCTCGCCGGCATGGTCCTGCGGCGGATCGAGACCAACATTCCCACGCTCGA
AGACCTCAAACTGCCCGACGTGTTGAAGAAGCTGGCGCTGACCAAGCGGGGGCTGGTGCTGTTCGTCGGGGCGACCGGTA
CTGGCAAATCCACGTCGCTGGCGGCAATGATTGGCTACCGCAACAAGAACACCAGTGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAGCTGCATCGTGACTCAGCGCGAGGTGGGCATCGATACGGAGTCGTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCTGACGTCATCCTCATCGGCGAAGTGCGGACCCGCGAGACGATGGATTATG
CCGTGGCGTTCGCCGAGACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCTGATCGGCAGCAGCAGGTATGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGGCGGCGAGCGGTGATCGAGGTGCTGATCAACACTCCGCTGGCCGCCGACCTGA
TCCGCAAAGGCGAGGTACATGAGCTCAAGGGGCTGATGAAGCGATCCACCGAGCAAGGCATGCAGACCTTCGATCAGGCG
CTCTACAACCTCTATGTGCAAGGCGAGATCACCTACGAGGATGCCTTGGCTCATGCCGATTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACAGCGAGCACCTCACCAGCGTGTCCCAGGGGCTGCGCCTGGAAGTCAGTGACG
ACGATCCAGGCTTCCGGATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.276

100

0.953
  pilU Acinetobacter baylyi ADP1

65.546

93.701

0.614
  pilU Vibrio cholerae strain A1552

60

91.864

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

88.976

0.386
  pilT Legionella pneumophila strain Lp02

41.493

87.927

0.365
  pilT Legionella pneumophila strain ERS1305867

41.493

87.927

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Neisseria gonorrhoeae MS11

40

90.551

0.362
  pilT Neisseria meningitidis 8013

40

90.551

0.362

Multiple sequence alignment