Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N028_RS03990 Genome accession   NZ_CP045799
Coordinates   861495..862712 (-) Length   405 a.a.
NCBI ID   WP_024638543.1    Uniprot ID   -
Organism   Pseudomonas syringae USA011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856495..867712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N028_RS03955 (N028_03955) - 857800..857907 (-) 108 WP_080377160.1 DUF3094 family protein -
  N028_RS03960 (N028_03960) - 857921..858454 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  N028_RS03965 (N028_03965) - 858451..859080 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  N028_RS03970 (N028_03970) - 859207..859668 (+) 462 WP_024638546.1 hypothetical protein -
  N028_RS03975 (N028_03975) yacG 859795..860004 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N028_RS03980 (N028_03980) coaE 860001..860624 (-) 624 WP_024638545.1 dephospho-CoA kinase -
  N028_RS03985 (N028_03985) pilD 860621..861493 (-) 873 WP_024638544.1 prepilin peptidase Machinery gene
  N028_RS03990 (N028_03990) pilC 861495..862712 (-) 1218 WP_024638543.1 type II secretion system F family protein Machinery gene
  N028_RS03995 (N028_03995) pilB 862715..864409 (-) 1695 WP_024638542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N028_RS04000 (N028_04000) - 864637..865065 (+) 429 WP_005732335.1 pilin -
  N028_RS04005 (N028_04005) - 865271..865816 (-) 546 WP_003317774.1 DUF1523 family protein -
  N028_RS04010 (N028_04010) - 865936..866934 (+) 999 WP_024638541.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44179.14 Da        Isoelectric Point: 10.2266

>NTDB_id=397300 N028_RS03990 WP_024638543.1 861495..862712(-) (pilC) [Pseudomonas syringae USA011]
MASKAVKVIVYTWEGVDKKGTKTSGELNGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRSLVESLKQEVSAGNSFATALRRKPEYFDELFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQSIFSSFGAELPAFTLMVIGLSDIVQKWWLAIVFAFFSGIFALK
RVYKKSQKFRDGLDRFLLKVPIIGPLIFKSSVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFKNAVQKVKQDVSTGM
QLNFSMRSTGVFPTLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=397300 N028_RS03990 WP_024638543.1 861495..862712(-) (pilC) [Pseudomonas syringae USA011]
ATGGCCAGCAAGGCAGTAAAAGTCATCGTTTACACTTGGGAAGGTGTCGACAAGAAAGGCACCAAAACCAGCGGCGAACT
GAACGGCCATAACCTGGCACTCGTAAAAGCACAGTTGCGCAAGCAAGGTATCAATCCCACCAAGGTACGCAAAAAATCCG
CGTCGATCTTCGGCAAAGGCAAGAAGATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTGCCATTGCTGCAGTCGTTTGACATCATCAGTGAGGGTGCTGAAAATCCGAATATGCGGTCGCTGGTCGAATC
TCTAAAGCAGGAGGTATCAGCCGGCAACAGCTTTGCCACCGCGTTACGAAGAAAACCAGAATACTTCGACGAGCTTTTCT
GTAACTTAGTGGATGCAGGGGAACAGGCGGGAGCACTTGAAAGCCTGCTGGACCGAGTAGCAAGCTACAAGGAGAAGACA
GAGAAGCTCAAGGCCAAGATAAAAAAGGCCATGACCTACCCGGCCGCAGTATTGATTGTGGCGATCATCGTTTCAGGTAT
TTTGCTTATAAAGGTTGTGCCACAGTTTCAGTCGATTTTTTCCAGCTTTGGTGCGGAACTGCCGGCCTTTACGTTAATGG
TTATCGGTCTTTCAGACATAGTGCAAAAGTGGTGGCTAGCTATCGTATTCGCTTTTTTTTCTGGCATTTTTGCACTTAAA
CGAGTCTACAAAAAATCTCAAAAATTTCGTGACGGACTTGATCGTTTCTTATTAAAAGTACCGATAATTGGCCCGTTAAT
TTTTAAATCCTCGGTCGCACGATACGCCCGAACCCTTTCAACTACGTTCGCCGCTGGTGTTCCGCTGGTAGAGGCTCTTG
ATTCGGTCGCTGGGGCCACTGGCAACGTAGTCTTCAAAAACGCGGTCCAAAAAGTTAAACAGGACGTTTCCACTGGTATG
CAGTTGAATTTCTCGATGCGTTCGACAGGTGTGTTCCCTACTTTAGCGATCCAAATGACCGCAATTGGTGAGGAATCAGG
CGCGTTAGACACCATGCTCGACAAGGTAGCGACCTACTACGAAGATGAGGTCGACAACATGGTCGACAACCTAACCAGCC
TGATGGAACCCATGATCATGGCTGTGCTGGGTGTCATCGTCGGCGGCCTTGTCATTGCGATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

72.84

100

0.728
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

51.88

98.519

0.511
  pilC Vibrio campbellii strain DS40M4

42.929

97.778

0.42
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilG Neisseria gonorrhoeae MS11

42.065

98.025

0.412
  pilG Neisseria meningitidis 44/76-A

42.065

98.025

0.412
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment