Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N028_RS03995 Genome accession   NZ_CP045799
Coordinates   862715..864409 (-) Length   564 a.a.
NCBI ID   WP_024638542.1    Uniprot ID   -
Organism   Pseudomonas syringae USA011     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857715..869409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N028_RS03955 (N028_03955) - 857800..857907 (-) 108 WP_080377160.1 DUF3094 family protein -
  N028_RS03960 (N028_03960) - 857921..858454 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  N028_RS03965 (N028_03965) - 858451..859080 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  N028_RS03970 (N028_03970) - 859207..859668 (+) 462 WP_024638546.1 hypothetical protein -
  N028_RS03975 (N028_03975) yacG 859795..860004 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N028_RS03980 (N028_03980) coaE 860001..860624 (-) 624 WP_024638545.1 dephospho-CoA kinase -
  N028_RS03985 (N028_03985) pilD 860621..861493 (-) 873 WP_024638544.1 prepilin peptidase Machinery gene
  N028_RS03990 (N028_03990) pilC 861495..862712 (-) 1218 WP_024638543.1 type II secretion system F family protein Machinery gene
  N028_RS03995 (N028_03995) pilB 862715..864409 (-) 1695 WP_024638542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N028_RS04000 (N028_04000) - 864637..865065 (+) 429 WP_005732335.1 pilin -
  N028_RS04005 (N028_04005) - 865271..865816 (-) 546 WP_003317774.1 DUF1523 family protein -
  N028_RS04010 (N028_04010) - 865936..866934 (+) 999 WP_024638541.1 ATP-grasp domain-containing protein -
  N028_RS04015 (N028_04015) - 866989..868128 (+) 1140 WP_024638540.1 trypsin-like peptidase domain-containing protein -
  N028_RS04020 (N028_04020) - 868195..869328 (+) 1134 WP_024638539.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62492.88 Da        Isoelectric Point: 6.3847

>NTDB_id=397301 N028_RS03995 WP_024638542.1 862715..864409(-) (pilB) [Pseudomonas syringae USA011]
MTDAVLTGLAKQLVLAELLTESIAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSESGLGNLEDVDLGLD
IEPVDGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLVISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=397301 N028_RS03995 WP_024638542.1 862715..864409(-) (pilB) [Pseudomonas syringae USA011]
ATGACTGATGCTGTCCTCACTGGTTTGGCCAAACAACTGGTCTTGGCCGAGCTACTTACTGAGTCCATCGCGCAACAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTCAGTTATCTGGTTCAGAACAAACTGGTCAAAAGTCTGACGCTGG
CAGAAATGGCTTCCGATCAGTTCGGCGTGCCGTTCATGGATCTCGCCAGCCTCGACAAGGAAAGCCAGCCTAAAGGTTTG
GTAAGTGAAAAGCTGGTTCGCCAGCATCACGCGCTGCCCCTTTGGCGACGCGGTAACAAGCTATTTATTGCAATTTCAGA
TCCGACCAATCATCAGGCGGTCACCGATATTCAGTTCAGTACTGGCCTGAATACCGAAGCGATTCTGGTAGAAGATGACA
AGCTAACCACTGCCATAGATCGTTTTTTTGACAGTGAAAGCGGTCTAGGTAATCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCGGTTGATGGCAAGGAAACCACTCTAGCTACACAGAATGACGCTGACGATGCTCCAGTCGTGAGATTCGTCAA
CAAGATGCTGATGGACGCTATCCGACTAGGGTCATCAGACCTGCACTTCGAGCCCTATGAAAAGATTTTTCGTGTGCGCT
TGCGTACTGACGGCATCCTGCATGAGGTAGCCAGACCTCCCATTCACTTGGCCAATCGTATTGCCGCACGCTTGAAAGTC
ATGGCCAGCCTGGATATCTCGGAGCGGCGCAAGCCGCAGGACGGACGCGTCAAATTACGTGTGTCCAAAACAAAAGCCAT
CGATTTTCGTATGAATACCTTACCCACGTTGTGGGGCGAGAAGATCGTAATGCGGATCCTCGACCCAACCAGTGCGCAGA
TGGGTATCGATGCGCTTGGCTATGAGCCAGATCAAAAGGCACTGTACCTAGAGGCGCTTAAATTGCCTCAAGGGATGATT
CTTGTGACTGGCCCTACAGGCTCAGGCAAGACTGTCTCTCTCTATACTGGCCTGAACATTCTCAACACTGTGGACATCAA
CATTTCCACCGCTGAAGACCCGGTTGAGATCAACCTGGAAGGCATCAATCAGGTCAATGTCAACCCGCGCCAGGGTCTGG
ACTTTTCCCAGGCCTTGCGCGCTTTTTTGCGTCAAGATCCCGACGTGATAATGGTCGGGGAGATACGAGACCTGGAAACG
GCAGAAATTGCCATCAAAGCCTCGCAGACCGGCCATATGGTGCTTTCCACACTGCACACCAACAGCGCAGCGGAAACCTT
GACCCGCCTGCATCATATGGGCGTTGCAGCGTTCAACATTGCTACAGCAATCAACCTCATCATTGCGCAAAGACTGGCAC
GTAAACTGTGTAGCCACTGCAAAAAAGAACTGGATATTCCTCGCGAGACACTGATTCAGGAAGGCTTTCCAGAAGCCAAA
ATTGGCACTTTCAAGATATACGGCCCGGTGGGCTGTGAGCACTGCAACGGGGGTTATCGAGGCAGGGTGGGCATTTACGA
AGTGGTCAAGAAAACACCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCACTGGTTATTTCCAGGCAGATGCGCA
AGGACGGTTTCAATGACCTTCGCACATCAGGGCTTTCGAAAGCCATGCAAGGCATTACCAGTCTTGAAGAAGTCAACCGA
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.201

100

0.585
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio parahaemolyticus RIMD 2210633

50.984

99.113

0.505
  pilB Vibrio cholerae strain A1552

50.802

99.468

0.505
  pilB Vibrio campbellii strain DS40M4

49.912

100

0.502
  pilF Neisseria gonorrhoeae MS11

48.307

99.468

0.481
  pilF Thermus thermophilus HB27

38.693

100

0.388

Multiple sequence alignment