Detailed information NTG0397299

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N028_RS03985
• Genome accession: NZ_CP045799
• Coordinates: 860621..861493 (-)
• Length: 290 a.a.
• NCBI ID: WP_024638544.1
• Uniprot ID: -
• Organism: Pseudomonas syringae USA011
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 855621..866493

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N028_RS03950 (N028_03950)	-	856406..857704 (-)	1299	WP_003430739.1	NAD(P)/FAD-dependent oxidoreductase	-
N028_RS03955 (N028_03955)	-	857800..857907 (-)	108	WP_080377160.1	DUF3094 family protein	-
N028_RS03960 (N028_03960)	-	857921..858454 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
N028_RS03965 (N028_03965)	-	858451..859080 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
N028_RS03970 (N028_03970)	-	859207..859668 (+)	462	WP_024638546.1	hypothetical protein	-
N028_RS03975 (N028_03975)	yacG	859795..860004 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
N028_RS03980 (N028_03980)	coaE	860001..860624 (-)	624	WP_024638545.1	dephospho-CoA kinase	-
N028_RS03985 (N028_03985)	pilD	860621..861493 (-)	873	WP_024638544.1	prepilin peptidase	Machinery gene
N028_RS03990 (N028_03990)	pilC	861495..862712 (-)	1218	WP_024638543.1	type II secretion system F family protein	Machinery gene
N028_RS03995 (N028_03995)	pilB	862715..864409 (-)	1695	WP_024638542.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N028_RS04000 (N028_04000)	-	864637..865065 (+)	429	WP_005732335.1	pilin	-
N028_RS04005 (N028_04005)	-	865271..865816 (-)	546	WP_003317774.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32021.24 Da
• Isoelectric Point: 8.3734

>NTDB_id=397299 N028_RS03985 WP_024638544.1 860621..861493(-) (pilD) [Pseudomonas syringae USA011]
MPLLDLLASSPLAFVITCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=397299 N028_RS03985 WP_024638544.1 860621..861493(-) (pilD) [Pseudomonas syringae USA011]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCTTGGCCTTCGTCATCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCAGAGAACTGCTCGGCC
TGCCTGCTGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCTTGGGAGAACCTGCCGGTGATCAGCTATCTACTGCTGCGCGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
TTACCCACTGGTCGAGCTGACCTGCGCAGTGTTATCGGCATACGTGGCCTGGCATTTCGGTTTCGGCTGGCAGGCGGCGG
CGATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTCACGTCGCTGAATGATGCGCTATGGGGTGCAGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.169	97.931	0.521
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.19	94.483	0.493
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476