Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GFU70_RS24340 Genome accession   NZ_CP045701
Coordinates   5621180..5622397 (+) Length   405 a.a.
NCBI ID   WP_058542680.1    Uniprot ID   A0AAJ3KWH8
Organism   Pseudomonas brassicacearum strain S-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5616180..5627397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS24320 (GFU70_24315) - 5617593..5617889 (+) 297 WP_058542683.1 DUF2845 domain-containing protein -
  GFU70_RS24325 (GFU70_24320) - 5618214..5618567 (-) 354 WP_058542682.1 BON domain-containing protein -
  GFU70_RS24330 (GFU70_24325) - 5618841..5619251 (-) 411 WP_058542685.1 pilin -
  GFU70_RS24335 (GFU70_24330) pilB 5619477..5621177 (+) 1701 WP_058542681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFU70_RS24340 (GFU70_24335) pilC 5621180..5622397 (+) 1218 WP_058542680.1 type II secretion system F family protein Machinery gene
  GFU70_RS24345 (GFU70_24340) pilD 5622399..5623268 (+) 870 WP_153388915.1 prepilin peptidase Machinery gene
  GFU70_RS24350 (GFU70_24345) coaE 5623268..5623891 (+) 624 WP_058542678.1 dephospho-CoA kinase -
  GFU70_RS24355 (GFU70_24350) yacG 5623888..5624088 (+) 201 WP_058542677.1 DNA gyrase inhibitor YacG -
  GFU70_RS24360 (GFU70_24355) - 5624274..5624489 (-) 216 WP_013694234.1 hypothetical protein -
  GFU70_RS24365 (GFU70_24360) - 5624553..5625239 (-) 687 WP_058542676.1 energy-coupling factor ABC transporter permease -
  GFU70_RS24370 (GFU70_24365) - 5625383..5626009 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -
  GFU70_RS24375 (GFU70_24370) - 5626006..5626533 (+) 528 WP_153388916.1 MOSC domain-containing protein -
  GFU70_RS24380 (GFU70_24375) - 5626586..5626759 (+) 174 WP_003205686.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44322.06 Da        Isoelectric Point: 10.0233

>NTDB_id=396109 GFU70_RS24340 WP_058542680.1 5621180..5622397(+) (pilC) [Pseudomonas brassicacearum strain S-1]
MAVKAVKTDVYTWEGKDRKGTKMTGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQQWWWMLLGGLAGAFFGVK
YALRRSERFRDWRDKWLLKLPLIGTLLYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQEVSTGM
QLNFSMRASGIFPNLAVQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=396109 GFU70_RS24340 WP_058542680.1 5621180..5622397(+) (pilC) [Pseudomonas brassicacearum strain S-1]
ATGGCGGTCAAGGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGACCGGCGAGCT
GACGGGCCAGAGCCCGGCCCTGGTCAAGGCACAGCTGCGCAAGCAAGGCATCAACCCGCAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCGACGATGCTCAAG
GCCGGCGTACCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCAGCGCTGCGCAAGTGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCCCTGGATACCCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCTGCGGCCGTGGTCCTCGTTGCCGCCGTGGTCACGGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTATTTTCCGGGTTCGGCGCGCAATTGCCGGCCTTCACGGTGATGG
TCATCGGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGATGTTGCTTGGCGGGTTGGCAGGGGCTTTTTTTGGTGTGAAG
TACGCGCTCAGGCGTTCGGAGCGCTTTCGCGACTGGCGCGACAAATGGCTGCTCAAGCTGCCGCTGATAGGCACCTTGCT
GTACAAGTCCGCCGTGGCTCGCTACGCCCGTACGCTTTCCACCACCTTCGCCGCCGGCGTGCCACTGGTGGAGGCCCTCG
ATTCGGTGTCTGGCGCCACCGGCAACGTGGTGTTCAAACGTGCCGTGCAACGCATCAAGCAGGAAGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGCGCCTCAGGGATCTTTCCGAACCTGGCGGTCCAGATGACCGCCATTGGCGAGGAATCCGG
CGCGCTGGACGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTTGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.548

98.272

0.585
  pilC Acinetobacter baumannii D1279779

57.353

100

0.578
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilG Neisseria gonorrhoeae MS11

43.284

99.259

0.43
  pilC Vibrio campbellii strain DS40M4

43.687

97.778

0.427
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria meningitidis 44/76-A

42.289

99.259

0.42
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment