Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GFU70_RS24335 Genome accession   NZ_CP045701
Coordinates   5619477..5621177 (+) Length   566 a.a.
NCBI ID   WP_058542681.1    Uniprot ID   A0AAJ3KVG1
Organism   Pseudomonas brassicacearum strain S-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5614477..5626177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS24315 (GFU70_24310) - 5614658..5617591 (+) 2934 WP_153388914.1 DUF748 domain-containing protein -
  GFU70_RS24320 (GFU70_24315) - 5617593..5617889 (+) 297 WP_058542683.1 DUF2845 domain-containing protein -
  GFU70_RS24325 (GFU70_24320) - 5618214..5618567 (-) 354 WP_058542682.1 BON domain-containing protein -
  GFU70_RS24330 (GFU70_24325) - 5618841..5619251 (-) 411 WP_058542685.1 pilin -
  GFU70_RS24335 (GFU70_24330) pilB 5619477..5621177 (+) 1701 WP_058542681.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFU70_RS24340 (GFU70_24335) pilC 5621180..5622397 (+) 1218 WP_058542680.1 type II secretion system F family protein Machinery gene
  GFU70_RS24345 (GFU70_24340) pilD 5622399..5623268 (+) 870 WP_153388915.1 prepilin peptidase Machinery gene
  GFU70_RS24350 (GFU70_24345) coaE 5623268..5623891 (+) 624 WP_058542678.1 dephospho-CoA kinase -
  GFU70_RS24355 (GFU70_24350) yacG 5623888..5624088 (+) 201 WP_058542677.1 DNA gyrase inhibitor YacG -
  GFU70_RS24360 (GFU70_24355) - 5624274..5624489 (-) 216 WP_013694234.1 hypothetical protein -
  GFU70_RS24365 (GFU70_24360) - 5624553..5625239 (-) 687 WP_058542676.1 energy-coupling factor ABC transporter permease -
  GFU70_RS24370 (GFU70_24365) - 5625383..5626009 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62670.63 Da        Isoelectric Point: 5.3093

>NTDB_id=396108 GFU70_RS24335 WP_058542681.1 5619477..5621177(+) (pilB) [Pseudomonas brassicacearum strain S-1]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNQLVQSRQIAEIASEHFGVALLDLNSLDKEAQPSGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQESIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGILREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAHALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKTAIEIPEEALLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=396108 GFU70_RS24335 WP_058542681.1 5619477..5621177(+) (pilB) [Pseudomonas brassicacearum strain S-1]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGTTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGCCGCATCCCCCTGGTCAGCTATCTGGTGCAGAACCAACTGGTGCAGAGCCGCCAGATCG
CCGAGATCGCCTCGGAGCATTTCGGCGTTGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAGCCCAACCCAGCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAATTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGAATCCAGCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
TTGGACATAGAATCGATCGACGACACCAGGCAGGAGTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTATGAAAAGATCTACCGGG
TGCGGGTGCGCACCGACGGCATATTGCGCGAAGTGGCCAGGCCGCCTATCCAGTTGGCCAACCGTATCGCCGCGCGCTTG
AAAGTCATGGCCAGCCTGGATATTTCCGAACGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACAGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAGG
GGCTGGATTTCGCCCATGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGTGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCTTGATCCGCCTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATTGCCCAGCGGT
TGGCGCGCAAACTGTGCAGCCACTGCAAGACGGCCATCGAGATCCCCGAGGAAGCCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACGTCGGGGCTGCTAAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.553
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.117

0.489
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilF Thermus thermophilus HB27

35.979

100

0.36

Multiple sequence alignment