Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GCK65_RS02430 Genome accession   NZ_CP045501
Coordinates   530400..531641 (-) Length   413 a.a.
NCBI ID   WP_011707570.1    Uniprot ID   A0KPV8
Organism   Aeromonas hydrophila strain Aer_Pi25.1HTAS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525400..536641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCK65_RS02400 (GCK65_02440) - 526241..527425 (+) 1185 WP_242797294.1 tetratricopeptide repeat protein -
  GCK65_RS02405 (GCK65_02445) mutT 527434..527847 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  GCK65_RS02410 (GCK65_02450) yacG 527908..528102 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  GCK65_RS02415 (GCK65_02455) zapD 528112..528834 (-) 723 WP_242797296.1 cell division protein ZapD -
  GCK65_RS02420 (GCK65_02460) coaE 528872..529486 (-) 615 WP_242797298.1 dephospho-CoA kinase -
  GCK65_RS02425 (GCK65_02465) pilD 529505..530377 (-) 873 WP_242797299.1 A24 family peptidase Machinery gene
  GCK65_RS02430 (GCK65_02470) pilC 530400..531641 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  GCK65_RS02435 (GCK65_02475) pilB 531757..533463 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GCK65_RS02440 (GCK65_02480) tapA 533470..533886 (-) 417 WP_042066959.1 type IVa pilus major pilin TapA -
  GCK65_RS02445 (GCK65_02490) nadC 534209..535066 (-) 858 WP_242797301.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GCK65_RS02450 (GCK65_02495) - 535072..535542 (-) 471 WP_242797303.1 retropepsin-like aspartic protease -
  GCK65_RS02455 (GCK65_02500) ampD 535690..536262 (+) 573 WP_242797305.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45422.58 Da        Isoelectric Point: 9.8637

>NTDB_id=394584 GCK65_RS02430 WP_011707570.1 530400..531641(-) (pilC) [Aeromonas hydrophila strain Aer_Pi25.1HTAS]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=394584 GCK65_RS02430 WP_011707570.1 530400..531641(-) (pilC) [Aeromonas hydrophila strain Aer_Pi25.1HTAS]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTGAATCGCAAGGGCCA
GAAGGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAGCAAGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCGCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTCATGGGCCAGATTGCTGCCGATGTGGAGACCGGCACGCCTATGTCCGAAGCGCTGCGCCGCCATCCCC
GCCACTTTGACGATCTCTACTGCGATCTAGTGGAGGCTGGCGAGCAGTCCGGCGCCCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCAGAAGCCCTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCACCATGGTCATATTGGT
GGCCATTGTCGTTACCTCCATCCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGACATCTTCAAGAGTTTCGGCGCCGAGC
TGCCCATCTTCACCCAGTTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATCTTTGGCGGCATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCATATTGACCAT
CCCCGTGGTCGGCATGATATTGCACAAGGCCGCCATGGCCCGCTTCGCCCGTACCCTCTCCACTACCTTCTCCGCCGGTA
TTCCGCTGGTGGATGCCTTGGTCTCTGCGGCAGGCGCCTCCGGCAACTATGTCTACCGCACTGCCGTCATGGCCATTCGT
AACGAGGTGGTGGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAACAGGAGGTAGACGACT
TGGTCGACGGCCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTTGCC
ATGTATCTGCCCATCTTCAAACTGGGATCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KPV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.348

96.126

0.446
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366

Multiple sequence alignment