Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GCK65_RS02435 Genome accession   NZ_CP045501
Coordinates   531757..533463 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain Aer_Pi25.1HTAS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526757..538463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCK65_RS02405 (GCK65_02445) mutT 527434..527847 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  GCK65_RS02410 (GCK65_02450) yacG 527908..528102 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  GCK65_RS02415 (GCK65_02455) zapD 528112..528834 (-) 723 WP_242797296.1 cell division protein ZapD -
  GCK65_RS02420 (GCK65_02460) coaE 528872..529486 (-) 615 WP_242797298.1 dephospho-CoA kinase -
  GCK65_RS02425 (GCK65_02465) pilD 529505..530377 (-) 873 WP_242797299.1 A24 family peptidase Machinery gene
  GCK65_RS02430 (GCK65_02470) pilC 530400..531641 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  GCK65_RS02435 (GCK65_02475) pilB 531757..533463 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GCK65_RS02440 (GCK65_02480) tapA 533470..533886 (-) 417 WP_042066959.1 type IVa pilus major pilin TapA -
  GCK65_RS02445 (GCK65_02490) nadC 534209..535066 (-) 858 WP_242797301.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GCK65_RS02450 (GCK65_02495) - 535072..535542 (-) 471 WP_242797303.1 retropepsin-like aspartic protease -
  GCK65_RS02455 (GCK65_02500) ampD 535690..536262 (+) 573 WP_242797305.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GCK65_RS02460 (GCK65_02505) pdhR 536538..537302 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=394585 GCK65_RS02435 WP_029302067.1 531757..533463(-) (pilB) [Aeromonas hydrophila strain Aer_Pi25.1HTAS]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=394585 GCK65_RS02435 WP_029302067.1 531757..533463(-) (pilB) [Aeromonas hydrophila strain Aer_Pi25.1HTAS]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGTTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAGCATCACGTCCTGCCCATCTACACCCAAGGGCACACCCTCTATATCGCTATGTCAGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAAAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCTGACGATGACGCGCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCAGTCAATCTGGCCAACCGCTTCTCAGCTCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCTCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGAGGGTCAATACCCTGCCCACCATGTGGGGTGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCCCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTACAGGTCAATCCCAAGG
CCGGGCTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTAGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTAGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGTATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATTAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment