Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIV01_RS12240 Genome accession   NZ_CP045350
Coordinates   2663354..2664676 (+) Length   440 a.a.
NCBI ID   WP_152431250.1    Uniprot ID   -
Organism   Vibrio aquimaris strain THAF100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2658354..2669676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV01_RS12210 (FIV01_12340) - 2658685..2659437 (-) 753 WP_152431244.1 thioredoxin fold domain-containing protein -
  FIV01_RS12215 (FIV01_12345) xerD 2659476..2660384 (-) 909 WP_152431245.1 site-specific tyrosine recombinase XerD -
  FIV01_RS12220 (FIV01_12350) fldB 2660536..2661057 (+) 522 WP_152431246.1 flavodoxin FldB -
  FIV01_RS12225 (FIV01_12355) ampD 2661142..2661687 (-) 546 WP_152431247.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FIV01_RS12230 (FIV01_12360) nadC 2661821..2662708 (+) 888 WP_152431248.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FIV01_RS20875 (FIV01_12365) - 2662917..2663354 (+) 438 WP_152431249.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIV01_RS12240 (FIV01_12370) pilB 2663354..2664676 (+) 1323 WP_152431250.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  FIV01_RS12245 (FIV01_12375) - 2664691..2665908 (+) 1218 WP_246210403.1 type II secretion system F family protein -
  FIV01_RS12250 (FIV01_12380) pilD 2665957..2666826 (+) 870 WP_152431251.1 A24 family peptidase Machinery gene
  FIV01_RS12255 (FIV01_12385) coaE 2666828..2667442 (+) 615 WP_152431252.1 dephospho-CoA kinase -
  FIV01_RS12260 (FIV01_12390) zapD 2667470..2668210 (+) 741 WP_152431253.1 cell division protein ZapD -
  FIV01_RS12265 (FIV01_12395) yacG 2668238..2668432 (+) 195 WP_152431254.1 DNA gyrase inhibitor YacG -
  FIV01_RS12270 (FIV01_12400) rplS 2668517..2668870 (-) 354 WP_009602110.1 50S ribosomal protein L19 -
  FIV01_RS12275 (FIV01_12405) trmD 2668912..2669655 (-) 744 WP_152431255.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 49547.86 Da        Isoelectric Point: 8.6984

>NTDB_id=393575 FIV01_RS12240 WP_152431250.1 2663354..2664676(+) (pilB) [Vibrio aquimaris strain THAF100]
MMSRLPRLFFNANVINEQQASSLYATEKTHLSSNHQEIDFHELDNLISNQVKVTDEAQRDESHTSCYIHQIISKAVRQKA
SDIHFEPYKHWFRIRFRCDGILVEVHQSPHQICKRLVARVKILSQLDISERRLPQDGRLTHRLNKSNDVHIRVSTLPTMW
GEKLVLRILDNQVTSLDLKMLGYSTAQRQIYEQALKKPQGLILITGPTGSGKTLSLYSGLSTLDSERLNISAAEDPIEIS
FSGINQVQIQPQIGFGFAEALRAFLRQDPDVIMLGEIRDKETADIATKAAQTGHLVLATLHTNSSTEAITRLKNIGIEPH
NIAASLCLVIAQRLLRRLCPLCKTPAPIPECLSKDNYSMFYQASTHGCSACNNGYLGRIGIYEMFSITPEISDSILKKDA
QHQLDKLAKKQGIKTLYQSGLERVNQGITSYAELRRILAP

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=393575 FIV01_RS12240 WP_152431250.1 2663354..2664676(+) (pilB) [Vibrio aquimaris strain THAF100]
ATGATGAGCCGACTTCCAAGATTATTTTTTAATGCCAATGTTATTAATGAACAACAAGCCAGTTCATTGTATGCCACAGA
AAAGACTCACCTCTCCTCCAACCACCAAGAGATAGATTTTCACGAACTCGATAATTTAATTAGCAATCAGGTAAAAGTAA
CAGATGAGGCGCAGCGAGATGAATCGCATACTAGTTGCTACATCCATCAGATCATCTCTAAAGCCGTTCGCCAAAAAGCA
TCTGATATTCACTTTGAACCCTATAAACACTGGTTTAGAATCCGGTTTCGCTGCGATGGGATATTAGTTGAGGTCCACCA
ATCACCACATCAAATTTGCAAAAGGCTTGTCGCCAGAGTCAAAATTTTATCGCAACTGGATATTTCTGAACGTCGCTTAC
CTCAAGATGGTCGTCTTACTCATAGATTAAACAAATCAAATGATGTACACATCCGTGTTTCCACTCTACCCACCATGTGG
GGCGAAAAGTTAGTATTAAGAATTTTAGATAACCAAGTCACTAGCTTAGATCTTAAAATGCTTGGCTATAGCACAGCACA
ACGCCAAATTTATGAGCAAGCATTAAAGAAACCTCAAGGTCTTATTCTTATTACTGGACCAACAGGGAGTGGTAAAACCT
TATCTTTATACAGCGGTTTATCTACACTCGATAGTGAACGTCTCAATATTTCTGCTGCGGAAGATCCAATTGAAATAAGC
TTCTCAGGTATCAATCAAGTTCAGATCCAGCCTCAAATTGGTTTTGGCTTCGCTGAAGCTTTACGGGCTTTTCTTCGTCA
GGATCCGGACGTCATCATGCTAGGGGAAATACGAGATAAAGAAACAGCAGATATAGCAACTAAAGCCGCTCAAACTGGCC
ATCTGGTTCTGGCGACATTGCACACCAACTCAAGCACTGAAGCAATCACTCGCCTAAAAAATATTGGCATAGAACCACAT
AACATCGCAGCCTCGCTTTGCCTAGTGATTGCCCAAAGATTACTCCGTCGTCTGTGCCCTTTGTGTAAGACTCCTGCACC
AATACCCGAATGTTTATCGAAAGACAATTACTCCATGTTTTATCAAGCCAGCACCCATGGATGCAGTGCCTGCAATAATG
GATATTTAGGCCGGATCGGTATCTATGAAATGTTTAGTATCACTCCAGAGATAAGCGATTCGATCTTAAAAAAAGACGCC
CAACACCAATTGGATAAACTAGCTAAAAAGCAAGGTATAAAAACGCTTTATCAGTCTGGATTAGAGAGAGTTAACCAAGG
AATAACTAGCTATGCCGAACTGCGACGTATACTAGCTCCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(70-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

54.353

96.591

0.525
  pilB Vibrio cholerae strain A1552

54.481

96.364

0.525
  pilB Vibrio campbellii strain DS40M4

53.791

95.909

0.516
  pilB Acinetobacter baumannii D1279779

48.471

96.591

0.468
  pilB Legionella pneumophila strain ERS1305867

45.721

100

0.461
  pilB Acinetobacter baylyi ADP1

47.307

97.045

0.459
  pilF Neisseria gonorrhoeae MS11

46.701

89.545

0.418
  pilB Haemophilus influenzae 86-028NP

43.541

95

0.414
  pilB Haemophilus influenzae Rd KW20

43.137

92.727

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

39.907

97.955

0.391
  pilF Thermus thermophilus HB27

43.75

87.273

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.25

90.909

0.375
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.078

93.636

0.366

Multiple sequence alignment