Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FIV01_RS01140 Genome accession   NZ_CP045350
Coordinates   245666..247024 (+) Length   452 a.a.
NCBI ID   WP_152429375.1    Uniprot ID   A0A5P9CFQ0
Organism   Vibrio aquimaris strain THAF100     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 240666..252024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV01_RS01120 (FIV01_01125) pilM 243100..244023 (+) 924 WP_152429371.1 pilus assembly protein PilM -
  FIV01_RS01125 (FIV01_01130) - 244013..244570 (+) 558 WP_152429372.1 PilN domain-containing protein -
  FIV01_RS01130 (FIV01_01135) pilO 244563..245153 (+) 591 WP_172971801.1 type 4a pilus biogenesis protein PilO -
  FIV01_RS01135 (FIV01_01140) - 245137..245655 (+) 519 WP_172971802.1 pilus assembly protein PilP -
  FIV01_RS01140 (FIV01_01145) pilQ 245666..247024 (+) 1359 WP_152429375.1 type IV pilus secretin PilQ Machinery gene
  FIV01_RS01145 (FIV01_01150) aroK 247268..247786 (+) 519 WP_152429376.1 shikimate kinase AroK -
  FIV01_RS01150 (FIV01_01155) aroB 247818..248906 (+) 1089 WP_152429377.1 3-dehydroquinate synthase -
  FIV01_RS01155 (FIV01_01160) - 248949..250442 (+) 1494 WP_152429378.1 AAA family ATPase -
  FIV01_RS01160 (FIV01_01165) - 250514..251341 (+) 828 WP_152429379.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49137.08 Da        Isoelectric Point: 4.8706

>NTDB_id=393557 FIV01_RS01140 WP_152429375.1 245666..247024(+) (pilQ) [Vibrio aquimaris strain THAF100]
MEKNRIGKSWPLLIGLFFYCQFVHAKEHSPSYQDNIQKVDISDRRLSVNFEDIPVRSVLQHIADYSDVNIVVSDSVSGSV
TLRLNDVYWSQLLDMILLVKGLDKRQIGDGLLVFKKSDANFIDNALVSEPQSLQSAIIAMRFANATEIAAILGGDAATSM
LSEHGAISVDTRTNALLIRDQATHIEAMKVIISALDVPVKQVQIEARIVSLSEGDVQELGVRWGINTIKGNYTLGGSIEN
NLSNIRLYSEGESSIDDFLNVDLSVASNTASTIAFQVAKLSSGVLLDLELSALQRESRAEVISSPRLITTNKQAAYIEQG
TEIPYLEAASSGATSVNFKKAVLSLKVTPQITADNRLILDLKVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNDGETIV
LGGIYQHSVSDAVDKVPLLGDIPLLGGLFRRSYQQMGKSELLIFVTPTLITP

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=393557 FIV01_RS01140 WP_152429375.1 245666..247024(+) (pilQ) [Vibrio aquimaris strain THAF100]
ATGGAGAAAAACAGAATTGGTAAAAGTTGGCCCTTATTGATTGGCTTATTTTTCTATTGTCAGTTTGTCCACGCGAAGGA
ACATTCCCCTTCTTATCAAGATAACATTCAAAAAGTGGATATCAGTGACCGCAGGCTATCGGTTAACTTTGAAGATATTC
CTGTACGGAGTGTGTTGCAGCATATAGCTGATTACAGCGACGTTAATATCGTGGTGTCTGATTCTGTATCTGGGTCTGTG
ACGTTAAGGCTCAACGATGTTTACTGGTCGCAACTGCTCGATATGATTCTGTTGGTTAAGGGCCTTGATAAACGCCAGAT
TGGTGATGGTTTGTTGGTATTTAAAAAATCAGATGCAAATTTCATTGATAATGCATTAGTGTCTGAACCGCAATCATTGC
AGTCTGCAATTATTGCCATGCGGTTTGCCAATGCCACAGAGATCGCCGCAATATTAGGAGGTGATGCGGCAACCTCCATG
CTGTCAGAGCACGGCGCTATTTCTGTAGATACAAGAACCAATGCGCTCTTGATTCGCGATCAAGCTACTCATATTGAGGC
GATGAAAGTCATTATCTCGGCACTCGATGTACCTGTAAAACAAGTACAAATAGAAGCGCGTATTGTGAGTTTGAGTGAAG
GTGATGTTCAAGAGCTGGGTGTTCGTTGGGGAATCAATACGATTAAGGGCAATTATACTCTTGGTGGTTCGATAGAAAAT
AATTTATCAAACATAAGATTATATTCAGAAGGTGAATCCAGCATTGATGACTTCCTTAATGTTGATCTTTCAGTTGCATC
TAACACTGCCTCGACAATTGCTTTTCAAGTCGCTAAATTGAGTAGTGGAGTCTTGCTTGATCTTGAGTTATCGGCGCTGC
AAAGAGAGTCAAGAGCTGAGGTTATTTCTAGCCCACGTTTGATTACTACCAACAAGCAAGCTGCGTACATAGAACAAGGT
ACAGAAATTCCTTATTTGGAAGCAGCTTCGAGTGGTGCTACCAGCGTTAACTTTAAAAAGGCGGTATTAAGCCTCAAGGT
AACGCCTCAGATAACCGCTGATAATCGCCTGATCCTTGACCTTAAGGTTACGCAAGATAGGCCGGGCCAAGTTGTGAAAA
CAGGCACAGGTGAAGCTGTCGCCATAGATACTCAACGCATTGGTACTCAGGTACTGGTTAATGATGGCGAGACGATAGTG
TTGGGCGGCATTTATCAACATAGTGTGTCAGATGCCGTTGATAAAGTGCCACTTTTGGGCGATATTCCGTTATTAGGAGG
GCTTTTTCGTCGGAGTTATCAGCAAATGGGAAAAAGTGAACTGCTTATTTTTGTCACACCGACTTTGATTACTCCTTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(292-450)

Bacterial type II/III secretion system short domain (Secretin_N)

(137-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9CFQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

63.615

94.248

0.6
  pilQ Vibrio cholerae strain A1552

60.771

97.566

0.593
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.771

97.566

0.593
  pilQ Legionella pneumophila strain Lp02

44.988

94.912

0.427
  pilQ Legionella pneumophila strain ERS1305867

44.755

94.912

0.425
  comQ Acinetobacter baylyi ADP1

39.827

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.931

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.931

100

0.389
  comE Haemophilus influenzae Rd KW20

39.062

99.115

0.387
  pilQ Pseudomonas aeruginosa PAK

39.954

95.796

0.383
  comE Haemophilus influenzae 86-028NP

41.542

88.938

0.369

Multiple sequence alignment