Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GA645_RS04970 Genome accession   NZ_CP045118
Coordinates   1048846..1050063 (-) Length   405 a.a.
NCBI ID   WP_152220496.1    Uniprot ID   -
Organism   Pseudomonas sp. SCB32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1043846..1055063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA645_RS04925 - 1044036..1044206 (-) 171 WP_152220482.1 DUF3094 domain-containing protein -
  GA645_RS04930 - 1044285..1044914 (-) 630 WP_152220483.1 DUF1780 domain-containing protein -
  GA645_RS04935 - 1045023..1045439 (+) 417 WP_152220486.1 GNAT family N-acetyltransferase -
  GA645_RS04940 - 1045442..1045909 (+) 468 WP_256676068.1 FAD/FMN-containing dehydrogenase -
  GA645_RS04945 - 1045906..1046595 (+) 690 WP_152220488.1 energy-coupling factor ABC transporter permease -
  GA645_RS04950 - 1046703..1046918 (+) 216 WP_152220490.1 hypothetical protein -
  GA645_RS04955 yacG 1047094..1047297 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  GA645_RS04960 coaE 1047294..1047902 (-) 609 WP_152220492.1 dephospho-CoA kinase -
  GA645_RS04965 pilD 1047968..1048840 (-) 873 WP_152220494.1 A24 family peptidase Machinery gene
  GA645_RS04970 pilC 1048846..1050063 (-) 1218 WP_152220496.1 type II secretion system F family protein Machinery gene
  GA645_RS04975 pilB 1050067..1051770 (-) 1704 WP_152220498.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA645_RS04980 - 1051996..1052511 (+) 516 WP_178119486.1 pilin -
  GA645_RS04990 - 1052825..1054720 (+) 1896 WP_152220500.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44210.78 Da        Isoelectric Point: 9.9670

>NTDB_id=392029 GA645_RS04970 WP_152220496.1 1048846..1050063(-) (pilC) [Pseudomonas sp. SCB32]
MAEKTLRTSIFTWEGTDRKGGKIKGELSGTNTALVKAQLRKQGINPTKVRKKGISLLGRGKRIKPIDIALFTRQMATMMG
SGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVAAGNSLANSLRKKPLYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKGKIKKAMTYPIAVVLVAIIVSAILLIKVVPQFQAVFANFGAELPAFTRMVISASEVLQQWWFIVLIVLFAIAFTLR
EAHRRSEKFRDSVDRSVLKVPVVGDIIYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVNKIKQDVSSGT
QLNFSMRTTGVFPSMAIQMAAIGEESGSLDAMLDKVASFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=392029 GA645_RS04970 WP_152220496.1 1048846..1050063(-) (pilC) [Pseudomonas sp. SCB32]
ATGGCGGAAAAAACATTACGGACCAGTATCTTCACCTGGGAAGGCACTGACCGAAAGGGCGGGAAGATCAAAGGGGAGCT
CTCGGGGACCAACACCGCGTTGGTCAAGGCCCAACTGCGCAAGCAGGGCATCAACCCCACCAAGGTCCGCAAGAAAGGCA
TCTCGCTGCTGGGTAGGGGCAAGCGGATCAAGCCGATCGATATCGCCCTGTTCACGCGGCAGATGGCGACCATGATGGGC
TCGGGTGTCCCCTTGCTGCAGTCTTTCGACATCATTGGCGAGGGCTTTGATAATCCGAACATGCGCAAGCTGGTGGATGA
GATCAAACAGGAAGTTGCCGCCGGTAACAGCCTGGCAAACTCCCTGCGCAAGAAGCCGCTGTACTTCGACGACCTGTACT
GCAACCTGGTCGATGCGGGCGAACAGTCGGGCGCCCTGGAAACCTTGCTTGACCGCGTAGCGACCTACAAGGAAAAGACC
GAGTCGCTCAAGGGCAAGATCAAGAAAGCCATGACCTACCCCATCGCGGTCGTGCTGGTGGCCATTATCGTTTCGGCCAT
TCTGCTGATCAAAGTGGTGCCGCAGTTCCAGGCGGTGTTTGCCAACTTCGGCGCGGAACTACCTGCATTTACCCGCATGG
TCATTTCCGCCTCGGAAGTGCTGCAGCAATGGTGGTTCATCGTCCTCATCGTCCTGTTCGCCATCGCCTTCACCCTGCGT
GAAGCCCATCGACGCTCGGAGAAGTTCCGGGATTCGGTCGATCGCTCTGTTTTGAAGGTGCCGGTCGTTGGCGACATCAT
CTACAAGTCCGCCGTAGCCCGTTACGCCCGAACGCTATCCACGACGTTCGCCGCGGGTGTTCCGCTGGTGGAAGCCCTGG
ACTCGGTATCCGGCGCGACCGGCAACGTGGTCTTCCGCAATGCGGTCAACAAGATCAAGCAGGATGTCTCCTCCGGTACC
CAATTGAACTTCTCCATGCGCACTACGGGAGTATTCCCGAGCATGGCCATTCAGATGGCAGCCATCGGCGAGGAGTCCGG
TTCGCTGGATGCCATGCTCGACAAGGTCGCGAGCTTCTACGAGGAAGAAGTCGACAACGCCGTCGATAACCTCACCACGC
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTATTGGTCGGCGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGCAACGTCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.247

100

0.802
  pilC Acinetobacter baumannii D1279779

59.95

98.025

0.588
  pilC Acinetobacter baylyi ADP1

58.621

100

0.588
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria meningitidis 44/76-A

45.088

98.025

0.442
  pilG Neisseria gonorrhoeae MS11

44.836

98.025

0.44
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Vibrio campbellii strain DS40M4

40.404

97.778

0.395
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment