Detailed information NTG0392028

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GA645_RS04965
• Genome accession: NZ_CP045118
• Coordinates: 1047968..1048840 (-)
• Length: 290 a.a.
• NCBI ID: WP_152220494.1
• Uniprot ID: -
• Organism: Pseudomonas sp. SCB32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1042968..1053840

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GA645_RS04925	-	1044036..1044206 (-)	171	WP_152220482.1	DUF3094 domain-containing protein	-
GA645_RS04930	-	1044285..1044914 (-)	630	WP_152220483.1	DUF1780 domain-containing protein	-
GA645_RS04935	-	1045023..1045439 (+)	417	WP_152220486.1	GNAT family N-acetyltransferase	-
GA645_RS04940	-	1045442..1045909 (+)	468	WP_256676068.1	FAD/FMN-containing dehydrogenase	-
GA645_RS04945	-	1045906..1046595 (+)	690	WP_152220488.1	energy-coupling factor ABC transporter permease	-
GA645_RS04950	-	1046703..1046918 (+)	216	WP_152220490.1	hypothetical protein	-
GA645_RS04955	yacG	1047094..1047297 (-)	204	WP_015475735.1	DNA gyrase inhibitor YacG	-
GA645_RS04960	coaE	1047294..1047902 (-)	609	WP_152220492.1	dephospho-CoA kinase	-
GA645_RS04965	pilD	1047968..1048840 (-)	873	WP_152220494.1	A24 family peptidase	Machinery gene
GA645_RS04970	pilC	1048846..1050063 (-)	1218	WP_152220496.1	type II secretion system F family protein	Machinery gene
GA645_RS04975	pilB	1050067..1051770 (-)	1704	WP_152220498.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GA645_RS04980	-	1051996..1052511 (+)	516	WP_178119486.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31771.70 Da
• Isoelectric Point: 7.0705

>NTDB_id=392028 GA645_RS04965 WP_152220494.1 1047968..1048840(-) (pilD) [Pseudomonas sp. SCB32]
MSVFDLLASTPLAFVLCALALGLLVGSFLNVVIHRLPRMMERDWQREARVALDLPEGEPLPTYNLILPNSQCPHCGHEIK
AWENIPVVSYLALGGKCSSCKAPISKRYPLVELACGALSAFIAWHYGFGWQAGAMLLLGWGLLAMSMIDVDHQLLPDALV
LPLLWLGLIVNYFGLFTHLGDALWGAVFGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGIITLRLRNAQTSTPIPFGPYLAIAGWIALLWGGQITATYLQFAGFH

Nucleotide

• Length: 873 bp

>NTDB_id=392028 GA645_RS04965 WP_152220494.1 1047968..1048840(-) (pilD) [Pseudomonas sp. SCB32]
ATGTCCGTCTTCGACCTCCTGGCCAGCACCCCGCTGGCCTTTGTTTTATGCGCGCTCGCACTCGGGCTTCTGGTCGGCAG
CTTCCTCAACGTGGTGATCCATCGCCTGCCGCGGATGATGGAACGCGACTGGCAGCGCGAGGCACGCGTGGCGCTGGATC
TGCCGGAAGGCGAGCCGCTGCCCACCTATAACCTGATCCTGCCGAACTCCCAGTGCCCGCACTGCGGCCATGAGATCAAG
GCCTGGGAAAACATCCCGGTCGTGAGCTACCTGGCACTGGGCGGCAAGTGCTCTTCGTGCAAGGCTCCGATCAGCAAGCG
CTATCCGCTGGTCGAGCTGGCGTGCGGCGCCCTCTCGGCCTTCATCGCCTGGCACTACGGTTTCGGCTGGCAAGCCGGCG
CCATGCTGCTGCTCGGCTGGGGCCTGCTGGCGATGAGCATGATCGACGTCGATCACCAGTTGCTGCCCGACGCGCTGGTC
CTGCCCCTGCTCTGGCTGGGGCTGATCGTCAACTACTTCGGATTGTTCACCCACCTGGGTGACGCGCTCTGGGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTACTGGCCATGCTGGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTAGGC
GCGGTGCTGGGCATCATCACCTTGCGCCTGCGCAACGCCCAGACCAGTACGCCGATCCCCTTCGGCCCCTATCTCGCCAT
CGCCGGCTGGATTGCGCTGCTCTGGGGTGGTCAAATAACCGCGACCTATCTGCAGTTCGCCGGTTTCCACTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.225	97.931	0.531
pilD	Vibrio campbellii strain DS40M4	52.817	97.931	0.517
pilD	Acinetobacter baumannii D1279779	52.632	91.724	0.483
pilD	Acinetobacter nosocomialis M2	52.632	91.724	0.483
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476