Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GA645_RS04975 Genome accession   NZ_CP045118
Coordinates   1050067..1051770 (-) Length   567 a.a.
NCBI ID   WP_152220498.1    Uniprot ID   -
Organism   Pseudomonas sp. SCB32     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045067..1056770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA645_RS04940 - 1045442..1045909 (+) 468 WP_256676068.1 FAD/FMN-containing dehydrogenase -
  GA645_RS04945 - 1045906..1046595 (+) 690 WP_152220488.1 energy-coupling factor ABC transporter permease -
  GA645_RS04950 - 1046703..1046918 (+) 216 WP_152220490.1 hypothetical protein -
  GA645_RS04955 yacG 1047094..1047297 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  GA645_RS04960 coaE 1047294..1047902 (-) 609 WP_152220492.1 dephospho-CoA kinase -
  GA645_RS04965 pilD 1047968..1048840 (-) 873 WP_152220494.1 A24 family peptidase Machinery gene
  GA645_RS04970 pilC 1048846..1050063 (-) 1218 WP_152220496.1 type II secretion system F family protein Machinery gene
  GA645_RS04975 pilB 1050067..1051770 (-) 1704 WP_152220498.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA645_RS04980 - 1051996..1052511 (+) 516 WP_178119486.1 pilin -
  GA645_RS04990 - 1052825..1054720 (+) 1896 WP_152220500.1 hypothetical protein -
  GA645_RS04995 - 1055082..1055648 (-) 567 WP_152220502.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62770.75 Da        Isoelectric Point: 5.9620

>NTDB_id=392030 GA645_RS04975 WP_152220498.1 1050067..1051770(-) (pilB) [Pseudomonas sp. SCB32]
MNDQVVLSGLARQLVQSQLLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLSSLDRESFPKD
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKLFESATGGLDDLADVDLE
GLDVENGNTERKDDPIGGDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLQEVAKPPVQLVSRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEAQKQLYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPSCRKEHDVPHETLRHEGFP
EDRIGTFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=392030 GA645_RS04975 WP_152220498.1 1050067..1051770(-) (pilB) [Pseudomonas sp. SCB32]
ATGAACGATCAAGTCGTCCTGTCCGGCCTTGCCCGGCAACTGGTGCAATCTCAACTGCTCGACGAGAAGAGCGCCCAGCA
GGCGCAGCAACAGGCCCAGCGCAACAAGCTGTCGCTGGTGACTTACCTCGTACAGAACAAACTGGTGAAAAGCCGGCCCC
TGACGGAGCTGGCGGCCGATCAATTTGGCGTCGCCTTCTGCGACCTGAGCAGCCTGGACCGGGAGAGCTTTCCCAAGGAC
CTGGTCAGCGAGAAACTGGTTCGCCAGCACCGCGTCCTTCCCCTTTGGCGGCGCGGCAACAAGCTCTTCGTCGGCCTCTC
GGACCCGACCAACCACCAGGCGATTACCGACGTCCAGTTCAGCACCGGCCTGACCACCGAGGCCATCCTGGTCGAGGACG
ACAAACTCGGCGATGCCATCGAGAAGCTCTTCGAAAGCGCCACCGGCGGGCTGGACGACCTGGCCGATGTCGATCTGGAG
GGCCTGGACGTCGAGAACGGCAACACCGAACGCAAGGATGATCCCATCGGTGGCGACGCCGATGACGCCCCCGTTGTCCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAGGGGCGGCTCGTCAGACCTGCACTTCGAACCCTACGAGAAGATCTACC
GCGTCCGCTTCCGTACCGACGGCATGCTTCAGGAAGTGGCCAAGCCGCCGGTCCAACTGGTCAGCCGCATCTCCGCGCGC
CTCAAGGTAATGGCGGGCCTGGATATTTCCGAGCGTCGCAAGCCGCAGGACGGCCGCATCAAGATGCGCGTATCCAAGAC
CAAGTCCATCGACTTCCGCGTCAATACCCTGCCCACGCTGTGGGGGGAAAAGATCGTGATGCGTATCCTCGACTCGGCCA
GCGCGCAGATGGGGATCGACGCCCTCGGCTACGAGGAGGCGCAGAAACAGCTCTATCTCAATGCACTGAAGCAGCCGCAA
GGCATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTATACCGGCCTGAACATCCTCAACACCCC
TGACATCAACATCTCCACCGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGAAGC
AAGGCCTGGACTTTGCCCAGGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGCGAC
CTGGAAACGGCGGAAATCGCCATCAAGGCGGCCCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCTGAACATGGGGGTTGCCTCCTTCAACCTCGCCACCTCCACCAACCTGATCATTGCCCAGC
GTCTGGCACGCAAGCTTTGCCCCAGCTGCCGGAAAGAGCATGACGTCCCACATGAAACGCTTCGCCACGAGGGTTTCCCG
GAAGACCGGATCGGTACGTTCAAGCTCTACGCGCCCGTTGGCTGCGAAAACTGCAAGAACGGTTACAAGGGGCGTGTAGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTACAGCGGATTATCATGGAAGAAGGCAATTCCATTCAGATCGCCGAAC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCGGGCCTGCTGAAGGCTATGCAGGGCATTACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.865

99.471

0.586
  pilB Acinetobacter baumannii D1279779

57.876

99.647

0.577
  pilB Legionella pneumophila strain ERS1305867

54.513

99.647

0.543
  pilB Vibrio parahaemolyticus RIMD 2210633

51.504

99.647

0.513
  pilB Vibrio cholerae strain A1552

50.613

100

0.51
  pilF Neisseria gonorrhoeae MS11

50.622

99.295

0.503
  pilB Vibrio campbellii strain DS40M4

50.353

99.824

0.503
  pilF Thermus thermophilus HB27

38.571

98.765

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.205

93.122

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

38.752

93.298

0.362

Multiple sequence alignment