Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   HKJ31_RS10250 Genome accession   NZ_CP052855
Coordinates   2263062..2263496 (+) Length   144 a.a.
NCBI ID   WP_004084597.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain Fillmore     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258062..2268496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKJ31_RS10230 (HKJ31_10430) coaE 2258160..2258783 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  HKJ31_RS10235 (HKJ31_10435) pilD 2258795..2259658 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  HKJ31_RS10240 (HKJ31_10440) pilC 2259665..2260867 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  HKJ31_RS10245 (HKJ31_10445) pilA/pilAI 2261308..2261754 (+) 447 WP_154128445.1 pilin Machinery gene
  HKJ31_RS10250 (HKJ31_10455) pilA/pilAI 2263062..2263496 (+) 435 WP_004084597.1 pilin Machinery gene
  HKJ31_RS10255 (HKJ31_10460) pilB 2264336..2266069 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKJ31_RS10260 (HKJ31_10465) - 2266163..2267575 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14980.38 Da        Isoelectric Point: 8.5024

>NTDB_id=388951 HKJ31_RS10250 WP_004084597.1 2263062..2263496(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Fillmore]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEITPSKAQAEIRIAEARPTTVSTDIGLRTPTARCAV
AVDIKTDAESTITCTLIGNTQVNGQTIKLSRAVDNAAGTGGLWTCTTTVAEALAPGGCTSTKKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=388951 HKJ31_RS10250 WP_004084597.1 2263062..2263496(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Fillmore]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATTACACCCAGCAAAGCACAAGCCG
AAATCCGTATTGCCGAGGCAAGACCAACAACTGTTTCGACCGATATCGGACTGCGCACCCCAACGGCTCGTTGCGCCGTT
GCTGTCGACATCAAGACCGATGCTGAAAGCACAATTACATGTACGCTTATTGGTAATACCCAAGTCAACGGTCAAACCAT
TAAGTTGAGTCGCGCCGTCGATAACGCCGCAGGCACAGGGGGTCTGTGGACCTGTACCACAACCGTTGCCGAAGCGTTGG
CACCAGGAGGCTGCACTTCGACCAAGAAGAGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.183

98.611

0.465
  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.143

97.222

0.458
  pilA Pseudomonas aeruginosa PAK

41.026

100

0.444
  pilA Acinetobacter baumannii strain A118

44.444

100

0.444
  pilA Vibrio cholerae strain A1552

39.49

100

0.431
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.49

100

0.431
  pilA Vibrio cholerae C6706

39.49

100

0.431
  pilA Vibrio parahaemolyticus RIMD 2210633

42.143

97.222

0.41
  pilA Ralstonia pseudosolanacearum GMI1000

37.179

100

0.403
  comP Acinetobacter baylyi ADP1

35.714

100

0.382