Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   FZF10_RS18030 Genome accession   NZ_CP043440
Coordinates   3462069..3463334 (-) Length   421 a.a.
NCBI ID   WP_149137368.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain MJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3457069..3468334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZF10_RS18005 - 3457303..3459345 (+) 2043 WP_149137365.1 OPT family oligopeptide transporter -
  FZF10_RS18010 - 3459375..3459566 (-) 192 WP_144201279.1 DNA gyrase inhibitor YacG -
  FZF10_RS18015 zapD 3459577..3460335 (-) 759 WP_144201281.1 cell division protein ZapD -
  FZF10_RS18020 coaE 3460536..3461144 (-) 609 WP_149137366.1 dephospho-CoA kinase -
  FZF10_RS18025 pilD 3461199..3462065 (-) 867 WP_149137367.1 A24 family peptidase Machinery gene
  FZF10_RS18030 pilG 3462069..3463334 (-) 1266 WP_149137368.1 type II secretion system F family protein Machinery gene
  FZF10_RS18035 pilF 3463371..3465098 (-) 1728 WP_149137369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FZF10_RS18045 ispB 3465495..3466424 (-) 930 WP_149137370.1 octaprenyl diphosphate synthase -
  FZF10_RS18055 rplU 3466962..3467273 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  FZF10_RS18060 rpmA 3467327..3467587 (+) 261 WP_061955320.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46461.70 Da        Isoelectric Point: 10.1574

>NTDB_id=384109 FZF10_RS18030 WP_149137368.1 3462069..3463334(-) (pilG) [Cupriavidus campinensis strain MJ1]
MATRAPAAGARTAAPSRAKSGRKAPTQYIFEWEAKDRKGKAFKGEQRAESQAEVVATLRKQGLTVVKMKKRKAARGKKIT
EKDIAYFTRQLSTMLKAGIPLLQSIDIIARGHANPNFTQLLSDIRFDIEAGSSMATAFRRQPKYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKTIALKSQIKSAMIYPISVLCVAFAVTVVLMLFVIPAFKGVFSSFGAALPAPTMVVINMSDFFVTY
WYLVIGIPIASIIFYLRARKTSEKVQRWHDKALLKLPIFGSLFRKAVIARWTRTLATMFAAGTPLVESMESVAGAAGNWL
YYDATREIEQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVAAISSLIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=384109 FZF10_RS18030 WP_149137368.1 3462069..3463334(-) (pilG) [Cupriavidus campinensis strain MJ1]
ATGGCGACGCGCGCACCAGCGGCGGGCGCCCGGACGGCAGCGCCGTCGCGGGCCAAATCAGGGCGGAAGGCGCCCACGCA
ATACATCTTCGAGTGGGAAGCCAAGGACCGCAAGGGCAAGGCCTTCAAGGGCGAGCAACGGGCCGAAAGCCAGGCGGAGG
TCGTCGCCACGCTGCGCAAGCAGGGGCTGACCGTCGTCAAGATGAAGAAGCGCAAGGCGGCGCGCGGCAAGAAGATCACC
GAGAAGGACATTGCGTACTTCACGCGCCAGCTCTCGACCATGCTCAAGGCCGGCATTCCGCTGCTGCAATCGATCGACAT
CATTGCGCGCGGCCACGCCAACCCCAATTTCACCCAACTGCTGTCCGACATCCGCTTCGACATCGAGGCCGGCAGCAGCA
TGGCCACGGCGTTCCGGCGCCAGCCGAAGTACTTCGACACGCTGTACTGCAACCTGATCGATGCCGGTGAGCAGGGCGGT
ATTCTCGATGCCCTGCTGGAGCGCCTTTCGCTCTACATGGAAAAGACCATCGCGCTGAAGAGCCAGATCAAGTCCGCGAT
GATCTATCCGATCTCGGTGCTCTGCGTGGCCTTCGCCGTGACCGTGGTGCTGATGCTGTTCGTGATTCCGGCGTTCAAGG
GCGTGTTCTCGAGCTTCGGCGCCGCCCTGCCTGCGCCGACGATGGTCGTGATCAACATGTCCGACTTCTTCGTCACGTAC
TGGTATCTGGTGATCGGGATACCGATTGCCTCGATCATCTTCTACCTCCGGGCGCGCAAGACCTCCGAAAAGGTCCAGCG
CTGGCACGACAAGGCGCTGCTGAAGCTGCCGATCTTCGGCAGCCTGTTCCGCAAGGCCGTGATCGCACGCTGGACCCGCA
CGCTGGCCACGATGTTCGCCGCCGGCACGCCGCTGGTGGAGTCGATGGAATCCGTGGCCGGCGCCGCCGGTAACTGGCTG
TATTACGATGCCACGCGGGAAATCGAACAGGCCGTACGGATCGGTACCAGCCTGACCAACGCGATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTCCAGATGACGCAGATCGGCGAGGAGTCCGGCGCGCTGGACAACATGCTGCTCAAGGTGGCGG
AATTCTACGAGCGCGAGGTCGATGACGCCGTGGCAGCCATCTCCAGCCTGATCGAGCCGCTGATCATCGTGATCCTGGGC
GTGCTGATTGGCGGCATGGTGGTGGCGATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.244

95.487

0.489
  pilG Neisseria meningitidis 44/76-A

50.995

95.487

0.487
  pilC Pseudomonas stutzeri DSM 10701

49.132

95.724

0.47
  pilC Acinetobacter baumannii D1279779

48.889

96.2

0.47
  pilC Acinetobacter baylyi ADP1

47.174

96.675

0.456
  pilC Legionella pneumophila strain ERS1305867

47.277

95.962

0.454
  pilC Vibrio campbellii strain DS40M4

38.462

95.724

0.368
  pilC Vibrio cholerae strain A1552

38.889

94.062

0.366
  pilC Thermus thermophilus HB27

36.779

98.812

0.363

Multiple sequence alignment