Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FZF10_RS18035 Genome accession   NZ_CP043440
Coordinates   3463371..3465098 (-) Length   575 a.a.
NCBI ID   WP_149137369.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain MJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3458371..3470098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZF10_RS18010 - 3459375..3459566 (-) 192 WP_144201279.1 DNA gyrase inhibitor YacG -
  FZF10_RS18015 zapD 3459577..3460335 (-) 759 WP_144201281.1 cell division protein ZapD -
  FZF10_RS18020 coaE 3460536..3461144 (-) 609 WP_149137366.1 dephospho-CoA kinase -
  FZF10_RS18025 pilD 3461199..3462065 (-) 867 WP_149137367.1 A24 family peptidase Machinery gene
  FZF10_RS18030 pilG 3462069..3463334 (-) 1266 WP_149137368.1 type II secretion system F family protein Machinery gene
  FZF10_RS18035 pilF 3463371..3465098 (-) 1728 WP_149137369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FZF10_RS18045 ispB 3465495..3466424 (-) 930 WP_149137370.1 octaprenyl diphosphate synthase -
  FZF10_RS18055 rplU 3466962..3467273 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  FZF10_RS18060 rpmA 3467327..3467587 (+) 261 WP_061955320.1 50S ribosomal protein L27 -
  FZF10_RS18065 cgtA 3467684..3468799 (+) 1116 WP_149137372.1 Obg family GTPase CgtA -
  FZF10_RS18070 proB 3468875..3469993 (+) 1119 WP_149137373.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62975.78 Da        Isoelectric Point: 8.0623

>NTDB_id=384110 FZF10_RS18035 WP_149137369.1 3463371..3465098(-) (pilF) [Cupriavidus campinensis strain MJ1]
MTLGLALAQSRRIAPALLAQLEQSAREKKSQLIDEIIGSGTMSAHDVAIFAADKYQLPLLDLAQYNLNRVPPALAGNREF
HAHRLLPLGRRENRLIVALSDPTNQAGVDAIKQKFNLPVETVVVEHDKLMKHVRAAGEALGTMKNVSPVTPTATKMIEYD
PVAAAASARKGTPGNEIDDAPVVRFLQKLLTEAFQRGASDLHFEPFEFFYRVRFRVDGVLQEVARPPLDIRDKIATRIKV
LSRLDISEKRVPQDGRMKLLLSLPKDKDAKEVIEKAIDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPAQKALLL
EVIKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDII
MVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKRAGDIPKPAL
LDAGFTEADTDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVLSLREAGLLKVK
QGVTSLEEVLATTNT

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=384110 FZF10_RS18035 WP_149137369.1 3463371..3465098(-) (pilF) [Cupriavidus campinensis strain MJ1]
ATGACACTAGGCCTTGCGCTCGCCCAGAGCCGACGGATCGCCCCCGCGCTACTCGCGCAGCTAGAGCAATCGGCCCGGGA
GAAAAAGTCGCAGCTGATTGACGAGATCATCGGCAGCGGCACCATGTCTGCCCATGACGTGGCCATCTTCGCCGCCGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTCAACCGCGTGCCGCCCGCGCTGGCCGGCAACCGCGAATTC
CACGCCCACCGCCTGCTGCCACTGGGCCGCCGTGAGAACCGGCTCATCGTGGCGCTGTCCGACCCGACCAACCAGGCCGG
CGTGGACGCCATCAAGCAGAAGTTCAACCTGCCGGTGGAAACCGTGGTGGTGGAGCACGACAAGCTGATGAAGCACGTGC
GCGCCGCCGGCGAGGCGCTCGGGACGATGAAAAACGTCTCGCCCGTGACGCCCACCGCCACCAAGATGATCGAGTACGAC
CCGGTGGCCGCTGCGGCCAGCGCCCGCAAGGGCACGCCGGGCAACGAAATCGACGATGCCCCCGTGGTGCGCTTCCTCCA
GAAGCTGCTGACCGAGGCATTCCAGCGCGGCGCATCGGACTTGCACTTCGAACCCTTCGAGTTCTTCTACCGCGTGCGAT
TCCGCGTGGACGGCGTGCTGCAGGAAGTGGCGCGTCCGCCGCTCGACATCCGCGACAAGATCGCCACCCGGATCAAGGTG
CTGTCGCGCCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGCTCTCGCTGCCCAAGGACAA
GGACGCCAAGGAAGTCATCGAGAAGGCGATCGACTTCCGTGTCTCCACGCTGCCTACGCTGTTCGGCGAGAAGATCGTGA
TGCGGATTCTGGAATCCTCGTCCGACAAGCTGGACATCGACCAGCTCGGCTACGAGCCCGCGCAGAAGGCGCTGCTGCTG
GAGGTCATCAAGCGGCCGTACGGGATGGTGCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTCTATACGTT
CCTGAACATGCTGAACCAGGGCGACATCAATATCTCCACGGCCGAGGATCCGGCGGAAATCCAGCTACCCGGCATCAACC
AGGTGAACGTGAACGACAAGGCCGGCCTGACCTTCGCGGCGGCGCTGAAGTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGATATCTCGATCAAGGCCGCCCAGACGGGCCACCTGGTGCTCTCCAC
GCTGCACACCAACGACGCCCCGACCACGCTCACGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCGTCGTCGG
TGCTGATGATCACGGCGCAGCGGCTGGCGCGCCGGCTCTGCACCTGCAAGCGCGCGGGCGACATTCCCAAGCCGGCCCTG
CTCGACGCCGGCTTCACCGAGGCCGACACCGACGGCAGCTGGCAGCCCTACCACCCGGTGGGCTGCGAGCGCTGCAACGG
CAGCGGCTACAAGGGCCGCTGCGGTATTTACCAGGTCATGCCGATCACGGAAGCGATGCAGCAGATCATTCTGTCGCACG
GCACGGCGCTGCAGATCGCCGAGCAGGCGCGCCGGGACGGCGTGCTATCTTTGCGGGAAGCTGGCCTGCTCAAGGTCAAA
CAGGGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACCAATACGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(188-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.552

100

0.52
  pilB Acinetobacter baumannii D1279779

51.579

99.13

0.511
  pilB Acinetobacter baylyi ADP1

51.878

97.217

0.504
  pilB Legionella pneumophila strain ERS1305867

48.288

96.522

0.466
  pilB Vibrio cholerae strain A1552

44.834

99.304

0.445
  pilB Vibrio campbellii strain DS40M4

45.841

94.087

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

45.387

94.261

0.428
  pilF Thermus thermophilus HB27

38.687

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.267

96.348

0.369

Multiple sequence alignment