Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HC658_RS12755 Genome accession   NZ_CP051466
Coordinates   2493006..2493416 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain UCMB5021     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2488006..2498416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC658_RS12725 (HC658_25140) - 2488118..2488258 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HC658_RS12730 (HC658_25150) yqeF 2488564..2489295 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  HC658_RS12735 (HC658_25160) cwlH 2489547..2490299 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HC658_RS12740 (HC658_25170) yqeD 2490486..2491112 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  HC658_RS12745 (HC658_25180) gnd 2491131..2491999 (-) 869 Protein_2472 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HC658_RS12750 (HC658_25190) yqeB 2492251..2492973 (+) 723 WP_010886572.1 hypothetical protein -
  HC658_RS12755 (HC658_25200) nucA/comI 2493006..2493416 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HC658_RS12760 - 2493612..2493959 (+) 348 Protein_2475 sigma-70 family RNA polymerase sigma factor -
  HC658_RS12765 (HC658_25210) spoIVCA 2493990..2495450 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  HC658_RS21060 - 2495408..2495586 (-) 179 Protein_2477 hypothetical protein -
  HC658_RS12770 (HC658_25220) arsC 2495941..2496360 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  HC658_RS12775 (HC658_25230) acr3 2496372..2497412 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  HC658_RS12780 (HC658_25240) arsK 2497435..2497875 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  HC658_RS12785 (HC658_25250) arsR 2497934..2498251 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=383908 HC658_RS12755 WP_009967785.1 2493006..2493416(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5021]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=383908 HC658_RS12755 WP_009967785.1 2493006..2493416(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain UCMB5021]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment