Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BSR23_RS02165 Genome accession   NZ_CP043421
Coordinates   406397..407413 (+) Length   338 a.a.
NCBI ID   WP_029862413.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain MVP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401397..412413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR23_RS02150 (BSR23_002150) - 401820..402548 (-) 729 WP_015297397.1 redoxin family protein -
  BSR23_RS02155 (BSR23_002155) oxyR 402719..403627 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  BSR23_RS02160 (BSR23_002160) - 403684..406254 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  BSR23_RS02165 (BSR23_002165) pilM 406397..407413 (+) 1017 WP_029862413.1 type IV pilus biogenesis protein PilM Machinery gene
  BSR23_RS02170 (BSR23_002170) pilN 407397..407975 (+) 579 WP_053815467.1 PilN domain-containing protein Machinery gene
  BSR23_RS02175 (BSR23_002175) pilO 407968..408561 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  BSR23_RS02180 (BSR23_002180) pilP 408551..409066 (+) 516 WP_025554074.1 pilus assembly protein PilP Machinery gene
  BSR23_RS02185 (BSR23_002185) pilQ 409098..410846 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  BSR23_RS02190 (BSR23_002190) aroK 411035..411553 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37734.23 Da        Isoelectric Point: 6.8321

>NTDB_id=383904 BSR23_RS02165 WP_029862413.1 406397..407413(+) (pilM) [Vibrio parahaemolyticus strain MVP1]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=383904 BSR23_RS02165 WP_029862413.1 406397..407413(+) (pilM) [Vibrio parahaemolyticus strain MVP1]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment