Detailed information
Bioinformatically predicted
Overview
| Name | pilA/pilAI | Type | Machinery gene |
| Locus tag | HB736_RS04850 | Genome accession | NZ_CP050328 |
| Coordinates | 1017312..1017779 (+) | Length | 155 a.a. |
| NCBI ID | WP_003161762.1 | Uniprot ID | - |
| Organism | Pseudomonas aeruginosa strain DVT419 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Genomic Context
Location: 1012312..1022779
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HB736_RS04825 (HB736_04830) | yacG | 1012482..1012682 (-) | 201 | WP_003094656.1 | DNA gyrase inhibitor YacG | - |
| HB736_RS04830 (HB736_04835) | coaE | 1012679..1013290 (-) | 612 | WP_003094654.1 | dephospho-CoA kinase | - |
| HB736_RS04835 (HB736_04840) | pilD | 1013287..1014159 (-) | 873 | WP_003161765.1 | type IV prepilin peptidase/methyltransferase PilD | Machinery gene |
| HB736_RS04840 (HB736_04845) | pilC | 1014160..1015377 (-) | 1218 | WP_003161763.1 | type II secretion system F family protein | Machinery gene |
| HB736_RS04845 (HB736_04850) | pilB | 1015381..1017081 (-) | 1701 | WP_003122078.1 | type IV-A pilus assembly ATPase PilB | Machinery gene |
| HB736_RS04850 (HB736_04855) | pilA/pilAI | 1017312..1017779 (+) | 468 | WP_003161762.1 | pilin | Machinery gene |
| HB736_RS04855 (HB736_04860) | - | 1017853..1019250 (+) | 1398 | WP_225319908.1 | O-antigen ligase | - |
| HB736_RS04865 (HB736_04870) | nadC | 1019431..1020279 (-) | 849 | WP_003161760.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
Sequence
Protein
Download Length: 155 a.a. Molecular weight: 15959.30 Da Isoelectric Point: 9.0145
>NTDB_id=378655 HB736_RS04850 WP_003161762.1 1017312..1017779(+) (pilA/pilAI) [Pseudomonas aeruginosa strain DVT419]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS
Nucleotide
Download Length: 468 bp
>NTDB_id=378655 HB736_RS04850 WP_003161762.1 1017312..1017779(+) (pilA/pilAI) [Pseudomonas aeruginosa strain DVT419]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA/pilAI | Pseudomonas stutzeri DSM 10701 |
45.679 |
100 |
0.477 |
| pilA | Pseudomonas aeruginosa PAK |
40.964 |
100 |
0.439 |
| pilA | Acinetobacter baumannii strain A118 |
43.312 |
100 |
0.439 |
| pilA | Ralstonia pseudosolanacearum GMI1000 |
37.427 |
100 |
0.413 |
| pilA | Vibrio cholerae O1 biovar El Tor strain E7946 |
43.262 |
90.968 |
0.394 |
| pilA | Vibrio cholerae strain A1552 |
43.262 |
90.968 |
0.394 |
| pilA | Vibrio cholerae C6706 |
43.262 |
90.968 |
0.394 |
| pilA/pilAII | Pseudomonas stutzeri DSM 10701 |
39.333 |
96.774 |
0.381 |
| pilA/pilA1 | Eikenella corrodens VA1 |
38.095 |
94.839 |
0.361 |