Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   FPZ22_RS09900 Genome accession   NZ_CP042218
Coordinates   2152327..2152770 (-) Length   147 a.a.
NCBI ID   WP_144892587.1    Uniprot ID   A0A518N5G8
Organism   Luteimonas granuli strain Gr-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2147327..2157770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPZ22_RS09885 (FPZ22_09885) - 2148350..2149852 (-) 1503 WP_144892580.1 hypothetical protein -
  FPZ22_RS09890 (FPZ22_09890) - 2149849..2150463 (-) 615 WP_144892583.1 hypothetical protein -
  FPZ22_RS09895 (FPZ22_09895) - 2150479..2152239 (-) 1761 WP_144892585.1 phosphoethanolamine transferase -
  FPZ22_RS09900 (FPZ22_09900) pilA2 2152327..2152770 (-) 444 WP_144892587.1 pilin Machinery gene
  FPZ22_RS09905 (FPZ22_09905) - 2153226..2153888 (+) 663 WP_168198981.1 class I SAM-dependent methyltransferase -
  FPZ22_RS09910 (FPZ22_09910) - 2153885..2154616 (+) 732 WP_144892591.1 methyltransferase domain-containing protein -
  FPZ22_RS09915 (FPZ22_09915) - 2154778..2155833 (+) 1056 WP_246120688.1 XRE family transcriptional regulator -
  FPZ22_RS09920 (FPZ22_09920) - 2155843..2156349 (+) 507 WP_144892594.1 hypothetical protein -
  FPZ22_RS09925 (FPZ22_09925) pilR 2156350..2157756 (-) 1407 WP_144892596.1 sigma-54 dependent transcriptional regulator Regulator

Sequence


Protein


Download         Length: 147 a.a.        Molecular weight: 14906.98 Da        Isoelectric Point: 7.7765

>NTDB_id=376064 FPZ22_RS09900 WP_144892587.1 2152327..2152770(-) (pilA2) [Luteimonas granuli strain Gr-4]
MKKMQQGFTLIELMIVVAIIAILAAIALPAYQDYLIKSRVSEVMIAASSARTSVSEAAAADTTNNMPTTVPIENQSSKYV
SGVVYAGSGTTGTITATAQNLGGTAAGNIVLTGTKAANGQVDWTCSGSIDAKYRPATCQAAAGSGSP

Nucleotide


Download         Length: 444 bp        

>NTDB_id=376064 FPZ22_RS09900 WP_144892587.1 2152327..2152770(-) (pilA2) [Luteimonas granuli strain Gr-4]
ATGAAGAAGATGCAGCAGGGCTTCACCCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCGGCGATCGC
GCTGCCGGCGTACCAGGACTACCTGATCAAGAGCCGCGTGTCGGAAGTAATGATCGCGGCTTCTTCGGCACGCACCAGCG
TCTCCGAAGCGGCCGCCGCTGATACTACCAACAACATGCCCACCACCGTCCCGATCGAGAATCAGTCCTCGAAGTATGTT
TCTGGCGTGGTCTATGCTGGTTCCGGCACCACCGGCACCATCACCGCCACTGCCCAAAATCTGGGCGGAACTGCGGCCGG
CAACATCGTCCTTACTGGCACCAAGGCTGCCAATGGCCAGGTCGACTGGACCTGCAGCGGCTCAATTGATGCGAAGTATC
GCCCCGCCACCTGCCAAGCCGCCGCTGGTAGCGGTAGCCCCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A518N5G8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.064

95.918

0.49

  pilA2 Legionella pneumophila strain ERS1305867

50.355

95.918

0.483

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.702

100

0.469

  pilA Ralstonia pseudosolanacearum GMI1000

41.566

100

0.469

  comP Acinetobacter baylyi ADP1

43.333

100

0.442

  pilA/pilA1 Eikenella corrodens VA1

38.312

100

0.401

  pilE Neisseria gonorrhoeae MS11

34.146

100

0.381

  pilE Neisseria gonorrhoeae strain FA1090

33.133

100

0.374

  pilA Pseudomonas aeruginosa PAK

35.099

100

0.361


Multiple sequence alignment