Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FO444_RS11765 Genome accession   NZ_CP041759
Coordinates   2293094..2294032 (-) Length   312 a.a.
NCBI ID   WP_211752889.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain IMAU11823     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2288094..2299032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FO444_RS11725 (FO444_11685) - 2288982..2289824 (-) 843 WP_017865154.1 metal ABC transporter substrate-binding protein -
  FO444_RS11730 (FO444_11690) - 2289821..2290258 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  FO444_RS11735 (FO444_11695) comGG 2290339..2290623 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  FO444_RS11740 (FO444_11700) comGF 2290662..2291087 (-) 426 WP_017865156.1 competence type IV pilus minor pilin ComGF Machinery gene
  FO444_RS11745 (FO444_11705) comGE 2291071..2291367 (-) 297 WP_017865157.1 competence type IV pilus minor pilin ComGE Machinery gene
  FO444_RS11750 (FO444_11710) comGD 2291339..2291770 (-) 432 WP_026138965.1 competence type IV pilus minor pilin ComGD Machinery gene
  FO444_RS11755 (FO444_11715) comGC 2291730..2292065 (-) 336 WP_032948139.1 competence type IV pilus major pilin ComGC Machinery gene
  FO444_RS11760 (FO444_11720) comGB 2292127..2293200 (-) 1074 WP_098403501.1 competence type IV pilus assembly protein ComGB Machinery gene
  FO444_RS11765 (FO444_11725) comGA 2293094..2294032 (-) 939 WP_211752889.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35656.91 Da        Isoelectric Point: 5.9445

>NTDB_id=373901 FO444_RS11765 WP_211752889.1 2293094..2294032(-) (comGA) [Lactococcus lactis subsp. lactis strain IMAU11823]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQMGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQCLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=373901 FO444_RS11765 WP_211752889.1 2293094..2294032(-) (comGA) [Lactococcus lactis subsp. lactis strain IMAU11823]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAAATGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGTGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.341

99.679

0.542
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.733

99.679

0.526
  comYA Streptococcus mutans UA159

51.603

100

0.516
  comYA Streptococcus mutans UA140

51.603

100

0.516
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.768

99.679

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.768

99.679

0.516
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.768

99.679

0.516
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.768

99.679

0.516
  comYA Streptococcus gordonii str. Challis substr. CH1

51.118

100

0.513

Multiple sequence alignment