Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FEE42_RS05895 Genome accession   NZ_CP040863
Coordinates   1154773..1155210 (+) Length   145 a.a.
NCBI ID   WP_059365849.1    Uniprot ID   -
Organism   Rodentibacter caecimuris strain G1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1149773..1160210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEE42_RS05865 (FEE42_05840) tadA 1150013..1150507 (-) 495 WP_256892853.1 tRNA adenosine(34) deaminase TadA -
  FEE42_RS05870 (FEE42_05845) - 1150517..1151368 (-) 852 WP_059365844.1 thymidylate synthase -
  FEE42_RS05875 (FEE42_05850) lgt 1151378..1152181 (-) 804 WP_259370290.1 prolipoprotein diacylglyceryl transferase -
  FEE42_RS05880 (FEE42_05855) - 1152190..1152987 (-) 798 WP_164027910.1 sulfite exporter TauE/SafE family protein -
  FEE42_RS05885 (FEE42_05860) rppH 1152991..1153572 (-) 582 WP_059365847.1 RNA pyrophosphohydrolase -
  FEE42_RS05890 (FEE42_05865) ampD 1154080..1154649 (-) 570 WP_164027911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FEE42_RS05895 (FEE42_05870) pilA 1154773..1155210 (+) 438 WP_059365849.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FEE42_RS05900 (FEE42_05875) pilB 1155207..1156595 (+) 1389 WP_059365850.1 GspE/PulE family protein Machinery gene
  FEE42_RS05905 (FEE42_05880) pilC 1156597..1157814 (+) 1218 WP_059365851.1 type II secretion system F family protein Machinery gene
  FEE42_RS05910 (FEE42_05885) pilD 1157814..1158503 (+) 690 WP_164027912.1 prepilin peptidase Machinery gene
  FEE42_RS05915 (FEE42_05890) coaE 1158513..1159133 (+) 621 WP_164027913.1 dephospho-CoA kinase -
  FEE42_RS05920 (FEE42_05895) yacG 1159126..1159332 (+) 207 WP_164027914.1 DNA gyrase inhibitor YacG -
  FEE42_RS05925 (FEE42_05900) - 1159329..1159601 (+) 273 WP_059365855.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14955.23 Da        Isoelectric Point: 8.4701

>NTDB_id=367722 FEE42_RS05895 WP_059365849.1 1154773..1155210(+) (pilA) [Rodentibacter caecimuris strain G1]
MKLISQPGLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAMSELLQASAPFKSDVELCVYGTGNTSNCSGGSNGVA
ANITTAKGYIKSVNTSAGVITVTGNGTLDGIEYTLTANGDKSKGVTWSTTCIGDTSLFPAGFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=367722 FEE42_RS05895 WP_059365849.1 1154773..1155210(+) (pilA) [Rodentibacter caecimuris strain G1]
ATGAAACTCATCTCTCAACCCGGACTGAAAAAAGGATTTACGCTCATTGAATTAATGATCGTGATTGCAATCATTGCAAT
TCTTGCCACTATCGCCATTCCGTCTTATCAAAATTATACCAAAAAGGCGGCAATGTCCGAATTGCTACAAGCCTCTGCCC
CATTTAAATCAGATGTCGAACTCTGTGTTTACGGCACCGGCAACACCTCAAATTGTAGTGGCGGCTCAAATGGTGTTGCC
GCCAACATTACCACCGCAAAAGGCTACATAAAAAGTGTAAACACGAGTGCAGGCGTAATAACTGTTACAGGTAACGGCAC
ATTAGATGGAATAGAATATACCCTCACCGCAAACGGCGATAAATCGAAAGGTGTGACTTGGTCAACCACCTGTATCGGCG
ATACGTCCTTATTTCCGGCAGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75

99.31

0.745
  pilA Haemophilus influenzae Rd KW20

72.222

99.31

0.717
  pilA Glaesserella parasuis strain SC1401

59.574

97.241

0.579
  comP Acinetobacter baylyi ADP1

43.262

97.241

0.421
  pilA2 Legionella pneumophila str. Paris

41.549

97.931

0.407
  pilA2 Legionella pneumophila strain ERS1305867

42.963

93.103

0.4
  pilA Vibrio campbellii strain DS40M4

39.041

100

0.393
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.889

99.31

0.386

Multiple sequence alignment