Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEE42_RS05900 Genome accession   NZ_CP040863
Coordinates   1155207..1156595 (+) Length   462 a.a.
NCBI ID   WP_059365850.1    Uniprot ID   A0AAJ3K5C8
Organism   Rodentibacter caecimuris strain G1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1150207..1161595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEE42_RS05870 (FEE42_05845) - 1150517..1151368 (-) 852 WP_059365844.1 thymidylate synthase -
  FEE42_RS05875 (FEE42_05850) lgt 1151378..1152181 (-) 804 WP_259370290.1 prolipoprotein diacylglyceryl transferase -
  FEE42_RS05880 (FEE42_05855) - 1152190..1152987 (-) 798 WP_164027910.1 sulfite exporter TauE/SafE family protein -
  FEE42_RS05885 (FEE42_05860) rppH 1152991..1153572 (-) 582 WP_059365847.1 RNA pyrophosphohydrolase -
  FEE42_RS05890 (FEE42_05865) ampD 1154080..1154649 (-) 570 WP_164027911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FEE42_RS05895 (FEE42_05870) pilA 1154773..1155210 (+) 438 WP_059365849.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FEE42_RS05900 (FEE42_05875) pilB 1155207..1156595 (+) 1389 WP_059365850.1 GspE/PulE family protein Machinery gene
  FEE42_RS05905 (FEE42_05880) pilC 1156597..1157814 (+) 1218 WP_059365851.1 type II secretion system F family protein Machinery gene
  FEE42_RS05910 (FEE42_05885) pilD 1157814..1158503 (+) 690 WP_164027912.1 prepilin peptidase Machinery gene
  FEE42_RS05915 (FEE42_05890) coaE 1158513..1159133 (+) 621 WP_164027913.1 dephospho-CoA kinase -
  FEE42_RS05920 (FEE42_05895) yacG 1159126..1159332 (+) 207 WP_164027914.1 DNA gyrase inhibitor YacG -
  FEE42_RS05925 (FEE42_05900) - 1159329..1159601 (+) 273 WP_059365855.1 GNAT family N-acetyltransferase -
  FEE42_RS05930 (FEE42_05905) rhlB 1159702..1160949 (+) 1248 WP_164027915.1 ATP-dependent RNA helicase RhlB -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52610.83 Da        Isoelectric Point: 5.7627

>NTDB_id=367723 FEE42_RS05900 WP_059365850.1 1155207..1156595(+) (pilB) [Rodentibacter caecimuris strain G1]
MTPALLEHRITAQNGEIFSISSELWERNKQQQALFLHYFSLPLKEEENRLWLGVDSLSNLSACETIAFVTGKLVEPVLLD
STQLKDLLQQLSPQSVQVEEQINFYHPQEQEHQEKNDDEPIIQLLNRIFEAALQKNASDIHLETLQDCFQVRFRIDGVLQ
PQAPISKNLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFAELGM
TESQQHTFQQALRQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELTGIIQSQINPQIGLDFSRLLRS
FLRQDPDIIMLGEIRDEESATMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIESSLLLVIAQRLVRKRCQKCG
QFSIDSCDCHQGYRGRIGVYQFLHRQQNNYQTDFANLRQSGLEKVKQGITDEAEIYRVLGGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=367723 FEE42_RS05900 WP_059365850.1 1155207..1156595(+) (pilB) [Rodentibacter caecimuris strain G1]
ATGACACCGGCATTACTTGAACATCGTATAACGGCACAAAACGGTGAGATCTTTTCGATCTCATCAGAGCTATGGGAACG
CAATAAACAGCAACAGGCTCTATTTTTGCACTATTTTTCCCTGCCTTTAAAAGAAGAGGAAAATCGTCTATGGCTCGGTG
TCGATTCTCTCTCCAATCTCTCAGCTTGCGAAACTATTGCTTTTGTTACCGGAAAATTGGTTGAACCGGTTTTGCTTGAC
AGCACACAACTCAAAGATCTTCTGCAACAACTTTCGCCGCAATCCGTACAAGTAGAAGAGCAAATAAATTTCTACCACCC
TCAAGAGCAAGAACATCAGGAAAAAAACGATGACGAACCTATTATCCAATTACTCAATCGTATTTTTGAGGCAGCACTAC
AAAAAAACGCTTCCGATATTCACTTAGAAACTTTACAGGATTGTTTTCAAGTGCGGTTTAGAATTGACGGGGTTTTACAA
CCCCAAGCACCGATTAGTAAAAATTTAGCCAATCGGTTAATTTCCCGATTAAAACTGCTTGCTAAACTTGATATCAGCGA
AACCCGATTACCACAAGACGGGCGTTTTCAATTTAAAACAACGTTCTCCGATATTCTTGATTTTCGCCTTTCTACACTGC
CTACCCATTGGGGCGAAAAAGTCGTACTTCGCGCACAACAAAATAAACCGGTGGAATTGAGTTTTGCAGAACTGGGCATG
ACGGAATCACAACAACACACTTTTCAGCAGGCACTCCGTCAACCTCAAGGTTTAATTCTGGTTACCGGCCCAACCGGTAG
CGGAAAAAGTATCTCCCTTTATACCGCACTTCAGTGGTTAAATACACCCGATAAACACATTATGACCGCAGAGGATCCCA
TCGAAATTGAATTAACGGGGATTATCCAAAGCCAAATTAATCCGCAAATCGGACTGGATTTTAGCCGCTTATTACGTTCA
TTTTTACGACAAGATCCCGACATTATTATGCTCGGTGAAATCCGAGATGAAGAAAGTGCGACAATGGCACTACGGGCGGC
GCAAACCGGCCATTTAGTGCTTTCAACCTTACATACAAATGATGCCATTTCTGCTATATCACGCTTACAACAACTTGGGA
TTCAACAACACGAAATAGAAAGTAGTTTATTACTGGTGATAGCCCAACGGCTTGTGCGAAAACGCTGCCAAAAGTGCGGT
CAATTTTCCATTGATTCTTGCGATTGCCATCAAGGTTATCGCGGCAGAATTGGCGTTTATCAATTCTTACATCGTCAACA
AAACAACTATCAAACCGATTTTGCCAATCTCCGTCAAAGCGGGTTAGAAAAAGTAAAACAAGGCATCACCGATGAGGCGG
AAATTTATCGTGTTTTGGGAGGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

84.416

100

0.844
  pilB Haemophilus influenzae Rd KW20

82.9

100

0.829
  pilB Glaesserella parasuis strain SC1401

58.206

98.918

0.576
  pilB Acinetobacter baumannii D1279779

39.147

100

0.437
  pilB Vibrio campbellii strain DS40M4

41.529

100

0.435
  pilB Vibrio cholerae strain A1552

41.031

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

41.042

100

0.426
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.416
  pilB Acinetobacter baylyi ADP1

38.586

100

0.413
  pilF Neisseria gonorrhoeae MS11

37.448

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.611

100

0.379

Multiple sequence alignment